Results 41 to 50 of about 927,051 (305)

Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence

Biochemistry and Biophysics Reports, 2019
While the binding of biotin by streptavidin does not appear to be cooperative in the traditional sense of altered binding strength, it has been suggested that it may be cooperative in terms of differential structural changes in the protein.
Mark J. Waner, James M. Hiznay, Anthony T. Mustovich, William Patton, Charles Ponyik, David P. Mascotti   +5 more
doaj   +1 more source

Altered modulation of lamin A/C-HDAC2 interaction and p21 expression during oxidative stress response in HGPS [PDF]

, 2018
Defects in stress response are main determinants of cellular senescence and organism aging. In fibroblasts from patients affected by Hutchinson-Gilford progeria, a severe LMNA-linked syndrome associated with bone resorption, cardiovascular disorders, and
Andrenacci, Davide, Cicchilitti, Lucia, Di Blasio, Anna Maria, Garofalo, Cecilia, Gentilini, Davide, Lattanzi, Giovanna, Mai, Antonello, Mattioli, Elisabetta, Piaggio, Giulia, Prencipe, Sabino, Remondini, Daniel, Scarano, Emanuela, Scotlandi, Katia, Valente, Sergio   +13 more
core   +2 more sources

Establishment of a graphene quantum dot (GQD) based steroid binding assay for the nuclear progesterone receptor (pgr)

Biochemistry and Biophysics Reports
Previously, we established a homogeneous assay for membrane progesterone receptor alpha (mPRα) ligands by conjugating semiconductor nanoparticles known as graphene quantum dots (GQDs) to mPRα. When mixed with a progesterone-BSA-fluorescein isothiocyanate
Md. Forhad Hossain, Shakhawat Hossain, Md. Maisum Sarwar Jyoti, Yuki Omori, Saokat Ahamed, Toshinobu Tokumoto   +5 more
doaj   +1 more source

Characterization of MaltOBP1, a Minus-C Odorant-Binding Protein, From the Japanese Pine Sawyer Beetle, Monochamus alternatus Hope (Coleoptera: Cerambycidae)

Frontiers in Physiology, 2020
Insect Odorant-Binding Proteins (OBPs) play crucial roles in the discrimination, binding and transportation of odorants. Herein, the full-length cDNA sequence of Minus-C OBP1 (MaltOBP1) from the Japanese pine sawyer beetle, Monochamus alternatus, was ...
Fangmei Zhang, Fangmei Zhang, Austin Merchant, Zhibin Zhao, Yunhui Zhang, Jing Zhang, Qingwen Zhang, Qinghua Wang, Xuguo Zhou, Xiangrui Li, Xiangrui Li   +10 more
doaj   +1 more source

A Fluorescence-Based Assay to Determine PDZ–Ligand Binding Thermodynamics

, 2021
Postsynaptic density-95, disks-large, and zonula occludens-1 (PDZ) domain interactions with cognate linear binding motifs (i.e., PDZ-binding motifs or PBMs) are important for many biological processes and can be pathological when disrupted. There are hundreds of PDZ-PBM interactions reported but few have been quantitatively determined.
Young Joo, Sun, Ernesto J, Fuentes
openaire   +3 more sources

Azadioxatriangulenium: a long fluorescence lifetime fluorophore for large biomolecule binding assay [PDF]

Methods and Applications in Fluorescence, 2013
Of the many optical bioassays available, sensing by fluorescence anisotropy has great advantages as it provides a sensitive, instrumentally simple, ratiometric method of detection. However, it is hampered by a severe limitation, as the emission lifetime of the label needs to be comparable to the correlation lifetime (tumbling time) of the biomolecule ...
Sørensen, Thomas Just, Thyrhaug, Erling, Szabelski, Mariusz, Luchowski, Rafal, Gryczynski, Ignacy, Gryczynski, Zygmunt, Laursen, Bo Wegge   +6 more
openaire   +4 more sources

Discovery of Stable and Selective Antibody Mimetics from Combinatorial Libraries of Polyvalent, Loop-Functionalized Peptoid Nanosheets. [PDF]

, 2019
The ability of antibodies to bind a wide variety of analytes with high specificity and high affinity makes them ideal candidates for therapeutic and diagnostic applications. However, the poor stability and high production cost of antibodies have prompted
Abate, Adam R, Cardiel, Joshua, Chen, Chun-Long, Chen, Yulin, Connolly, Michael D, Dehigaspitiya, Dilani C, Grzincic, Elissa M, Jian, Tengyue, Karbaschi, Mohsen, Kim, Jae Hong, Kim, Samuel C, Kirshenbaum, Kent, Kline, Mark A, Liu, Jianfang, Murray, Daniel J, Ren, Gang, Tresca, Blakely W, Udumula, Venkatareddy, Yun, Lisa, Zuckermann, Ronald N   +19 more
core   +1 more source

A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments

Cell Reports: Methods, 2023
Summary: Due to the enhanced labeling capability of maleimide-based fluorescent probes, lysine-cysteine-lysine (KCK) tags are frequently added to proteins for visualization. In this study, we employed an in vitro single-molecule DNA flow-stretching assay
Miranda Molina, Lindsey E. Way, Zhongqing Ren, Qin Liao, Bianca Guerra, Brandon Shields, Xindan Wang, HyeongJun Kim   +7 more
doaj   +1 more source

Biosensors for cardiac biomarkers detection: a review [PDF]

, 2012
The cardiovascular disease (CVD) is considered as a major threat to global health. Therefore, there is a growing demand for a range of portable, rapid and low cost biosensing devices for the detection of CVD.
Gurbuz, Yasar, Gürbüz, Yaşar, Kolkar Mohammed, Javed Hussain Niazi, Qureshi, Anjum   +3 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source