Results 71 to 80 of about 927,051 (305)

Characterization of the chemosensory protein EforCSP3 and its potential involvement in host location by Encarsia formosa

Journal of Integrative Agriculture, 2023
Chemosensory proteins (CSPs) perform several functions in insects. This study performed the gene expression, ligand-binding, and molecular docking assays on the EforCSP3 identified in the parasitoid wasp Encarsia formosa, to determine whether EforCSP3 ...
Ke WANG, Yan-yan HE, You-jun ZHANG, Zhao-jiang GUO, Wen XIE, Qing-jun WU, Shao-li WANG   +6 more
doaj   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Screening of G-quadruplex DNA ligands by fluorescence detection of peptide displacement

European Journal of Medicinal Chemistry Reports
G-quadruplexes (G4s) are noncanonical DNA/RNA structures involved in key cellular processes, and their interactions with proteins are emerging as therapeutic targets.
Valentina Arciuolo, Simona Marzano, Rossella Buono, Nicola Grasso, Anna Di Porzio, Antonio Randazzo, Bruno Pagano, Jussara Amato   +7 more
doaj   +1 more source

Structure, Binding Characteristics, and 3D Model Prediction of a Newly Identified Odorant-Binding Protein from the Cotton Bollworm, Helicoverpa armigera (Hübner)

Journal of Integrative Agriculture, 2012
The full-length sequence of the odorant binding protein 5 gene, HarmOBP5, was obtained from an antennae cDNA library of cotton bollworm, Helicoverpa armigera (Hübner). The cDNA contains a 444 bp open reading frame, encoding a protein with 147 amino acids,
Tian-tao ZHANG, Wei-xuan WANG, Shao-hua GU, Zi-ding ZHANG, Kong-ming WU, Yong-jun ZHANG, Yu-yuan GUO   +6 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Outer membrane protein U (OmpU) mediates adhesion of Vibrio mimicus to host cells via two novel N-terminal motifs.

PLoS ONE, 2015
Vibrio mimicus (V.mimicus) is a causative agent of ascites disease in aquatic animals. Our previous studies have demonstrated that the outer membrane protein U (OmpU) from V.mimicus is an immunoprotective antigen with six immunodominant linear B-cell ...
Xueqin Liu, Huihui Gao, Nin Xiao, Yan Liu, Jinnian Li, Lin Li   +5 more
doaj   +1 more source

Molecular and Functional Characterization of Odorant Binding Protein 7 From the Oriental Fruit Moth Grapholita molesta (Busck) (Lepidoptera: Tortricidae)

Frontiers in Physiology, 2018
Odorant-binding proteins (OBPs) are widely and abundantly distributed in the insect sensillar lymph and are essential for insect olfactory processes. The OBPs can capture and transfer odor molecules across the sensillum lymph to odorant receptors and ...
Xiu-Lin Chen, Xiu-Lin Chen, Guang-Wei Li, Xiang-Li Xu, Jun-Xiang Wu   +4 more
doaj   +1 more source

Miniaturization of Fluorescence Polarization Receptor-Binding Assays Using CyDye-Labeled Ligands [PDF]

SLAS Discovery, 2003
Fluorescence polarization (FP) is an established technique for the study of biological interactions and is frequently used in the high-throughput screening (HTS) of potential new drug targets. This work describes the miniaturization of FP receptor assays to 1536-well formats for use in HTS. The FP assays were initially developed in 384-well microplates
Alison, Harris, Sarah, Cox, Dougal, Burns, Christopher, Norey   +3 more
openaire   +2 more sources

PICALM::MLLT10 translocated leukemia

FEBS Letters, EarlyView.
This comprehensive review of PICALM::MLLT10 translocated acute leukemia provides an in‐depth review of the structure and function of CALM, AF10, and the fusion oncoprotein (1). The multifaceted molecular mechanisms of oncogenesis, including nucleocytoplasmic shuttling (2), epigenetic modifications (3), and disruption of endocytosis (4), are then ...
John M. Cullen, Antonia C. Nakatsugawa, Natalie Barton, Henry Haines, Gary S. Stein, Janet L. Stein, Daniel S. Wechsler, Jessica L. Heath   +7 more
wiley   +1 more source

Fluorescence anisotropy microplate assay for analysis of steroid receptor-DNA interactions

BioTechniques, 2004
To analyze the interactions of steroid/nuclear hormone receptors with their DNA response elements, we used ultra low-volume microplates to develop a simple and rapid fluorescence anisotropy assay. The novel fluorescence anisotropy microplate assay (FAMA)
Stanley Y. Wang, Bonnie S. Ahn, Rebecca Harris, Steven K. Nordeen, David J. Shapiro   +4 more
doaj   +1 more source