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Fluorescence Lifetime Imaging Microscopy
Current Protocols in Cell Biology, 2004AbstractFluorescent lifetime imaging microscopy is a powerful tool to enhance the contrast in images of biological samples and to investigate the local environment of a fluorochrome. FLIM allows the detection of protein‐protein interactions and their biochemical state by the quantitative detection of Förster resonance energy transfer (FRET) between ...
Alessandro, Esposito, Fred S, Wouters
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Fluorescence lifetime-resolved imaging
Photosynthesis Research, 2009This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the ...
Yi-Chun, Chen, Robert M, Clegg
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Fluorescence lifetime imaging of coral fluorescent proteins
Microscopy Research and Technique, 2007AbstractCorals, like many other coelenterates, contain fluorescent pigments that show considerable homology with the well known green fluorescent protein of the jellyfish Aequoria. In corals, unlike jellyfish, multiple proteins are present and the range of excitations and emissions suggest the possibility of energy transfer.
Guy, Cox, Mikhail, Matz, Anya, Salih
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Fluorescence Lifetime Imaging Microscopy
2007Publisher Summary Fluorescence lifetime imaging microscopy (FLIM) produces spatially resolved images of fluorophore lifetime (the property describing how rapidly fluorescence decays), providing another dimension of information for visualizing fluorophores and an additional source of contrast.
Ching-Wei, Chang +2 more
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Video rate fluorescence lifetime imaging and fluorescence lifetime endoscopy
SPIE Proceedings, 2005We report real-time (video-rate) fluorescence lifetime imaging and its application to tissue autofluorescence and endoscopy, demonstrating FLIM of unstained ex vivo tissue at update rates of 5.5 Hz through a flexible endoscope.
James McGinty +8 more
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Rapid hyperspectral fluorescence lifetime imaging
Microscopy Research and Technique, 2007AbstractWe report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high‐speed FLIM technology in a line‐scanning microscope. We demonstrate the acquisition of whole‐field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to ...
Pieter, De Beule +13 more
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2014
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation ...
Suhling, Klaus +11 more
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Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation ...
Suhling, Klaus +11 more
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Multispectral fluorescence lifetime imaging by TCSPC
Microscopy Research and Technique, 2007AbstractWe present a fluorescence lifetime imaging technique with simultaneous spectral and temporal resolution. The technique is fully compatible with the commonly used multiphoton microscopes and nondescanned (direct) detection. An image of the back‐aperture of the microscope lens is projected on the input of a fiber bundle.
Wolfgang, Becker +2 more
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Confocal Fluorescence Lifetime Imaging
1996The optical microscope, arguably the most mature of all laboratory instruments, is currently enjoying a period of intense development that is opening up many new application areas. These developments have been made possible by the use of new optical arrangements, sophisticated light sources and detectors and by the application of novel fluorescence ...
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Fluorescence lifetime imaging of intracellular calcium
Journal of Fluorescence, 1993Fluorescence lifetime imaging microscopy (FLIM) is a new methodology for studying the spatial and temporal dynamics of macromolecule, molecules, and ions in living cells. In FLIM image contrast is derived from the mean fluorescence lifetime at each point in a two-dimensional image.
H, Szmacinski +4 more
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