Results 221 to 230 of about 41,989 (251)

Fluorescence lifetime imaging in turbid media

Optics Letters, 1996
The lifetime of a f luorophore generally varies in different environments, making the molecule a sensitive indicator of tissue oxygenation, pH, and glucose. However, lifetime measurements are complicated when the f luorophore is embedded in an optically thick, highly scattering medium such as human tissue.
M A, O'Leary, D A, Boas, X D, Li, B, Chance, A G, Yodh   +4 more
openaire   +2 more sources

Fourier Multiplexed Fluorescence Lifetime Imaging

2021
Fluorescence lifetime imaging microscopy (FLIM) is a widely used functional imaging method in bioscience. Fourier multiplexed FLIM (FmFLIM), a frequency-domain lifetime measurement method, explores the principle of Fourier (frequency) multiplexing to achieve parallel lifetime detection on multiple fluorescence labels.
openaire   +2 more sources

Fluorescence Lifetime Imaging of Cancer In Vivo

2016
Optical imaging of fluorescent reporters in animal models of cancer has become a common tool in oncologic research. Fluorescent reporters including fluorescent proteins, organic dyes, and inorganic photonic materials are used in fluorescence spectroscopy, microscopy, and whole body preclinical imaging. Fluorescence lifetime imaging provides additional,
Owen, Peng, Walter J, Akers
openaire   +2 more sources

Fluorescence Lifetime Imaging Microscopy (FLIM)

2005
Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems have been implemented both in the frequency domain, using sinusoidally intensity-modulated excitation light and modulated detectors, and in the time domain, using pulsed excitation ...
Erik B, van Munster, Theodorus W J, Gadella   +1 more
openaire   +2 more sources

Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging Microscopy

Nephron Experimental Nephrology, 2006
With few and commercially available add-ons, both confocal and full-field fluorescence microscopes can be adapted to provide more information on the biological sample of interest. In this review we discuss the possibilities offered by two additional functionalities to fluorescence microscopes, fluorescence correlation spectroscopy (FCS) and ...
Sophia Y, Breusegem, Moshe, Levi, Nicholas P, Barry   +2 more
openaire   +2 more sources

Fluorescence lifetime imaging: an emerging technique in fluorescence microscopy

Chromosome Research, 1996
Fluorescence microscopy is an important tool for biological research, in part because of the extremely high detection sensitivity that can be achieved, but also because fluorescent molecules can be used as probes on account of their environmental responsiveness, for example to measure intracellular pH or metal ion concentration.
C G, Morgan, A C, Mitchell
openaire   +2 more sources

Fluorescence lifetime-resolved imaging: measuring lifetimes in an image.

Methods in enzymology, 2003
We have given an overview of what one can gain by lifetime-resolved imaging and reviewed the major issues concerning lifetime-resolved measurements and FLI instrumentation. Instead of giving diverse selected examples, we have discussed the underlying basic pathways of deexcitation available to the molecules in the excited state.
Robert M, Clegg, Oliver, Holub, Christopher, Gohlke   +2 more
openaire   +1 more source

Fluorescence Lifetime Imaging Microscopy: Two‐Dimensional Distribution Measurement of Fluorescence Lifetime

2006
A newly developed fluorescence lifetime imaging microscopy (FLIM) system has combined the high-temporal resolution of a streak camera with the high-spatial resolution of a microscope to obtain a two-dimensional distribution of fluorescence lifetimes within living cells. The temporal resolution is as short as 20 ps.
Masanobu, Fujiwara, William, Cieslik
openaire   +2 more sources

Fluorescence Lifetime Imaging in Scanning Microscopy

2006
Third ...
Gerritsen, H.C., Draaijer, A., Heuvel, D.J. van den, Agronskaia, A.V.   +3 more
openaire   +3 more sources

Deep learning enhanced fast fluorescence lifetime imaging with a few photons

Optica, 2023
David D-U Li
exaly