Protein Synthesis Rate Assessment by Fluorescence Recovery after Photobleaching (FRAP) [PDF]
Bio-Protocol, 2017 Currently available biochemical methods cannot be applied to monitor protein synthesis in specific cells or tissues, in live specimens. Here, we describe a non-invasive method for monitoring protein synthesis in single cells or tissues with intrinsically
Nikos Kourtis, Nektarios Tavernarakis
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Determining vitreous viscosity using fluorescence recovery after photobleaching. [PDF]
PLoS ONE, 2022 PurposeVitreous humor is a complex biofluid whose composition determines its structure and function. Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
Nishanthan Srikantha +6 more
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The Utility of Fluorescence Recovery after Photobleaching (FRAP) to Study the Plasma Membrane [PDF]
Membranes, 2023 The plasma membrane of mammalian cells is involved in a wide variety of cellular processes, including, but not limited to, endocytosis and exocytosis, adhesion and migration, and signaling.
Charles A. Day, Minchul Kang
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Using Fluorescence Recovery After Photobleaching data to uncover filament dynamics. [PDF]
PLoS Computational Biology, 2022 Fluorescence Recovery After Photobleaching (FRAP) has been extensively used to understand molecular dynamics in cells. This technique when applied to soluble, globular molecules driven by diffusion is easily interpreted and well understood.
J C Dallon +5 more
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Monitoring condensate dynamics in S. cerevisiae using fluorescence recovery after photobleaching [PDF]
STAR Protocols, 2022 Summary: This protocol describes the use of fluorescence recovery after photobleaching (FRAP) to investigate the dynamics of Matrin-3 (MATR3) condensates in live budding yeast.
Macy L. Sprunger, Meredith E. Jackrel
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Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM [PDF]
Molecules, 2012 Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Gregor P. C. Drummen +2 more
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Nasal epithelial cell culture fluorescence recovery after photobleaching predicts cystic fibrosis therapeutic response [PDF]
ERJ Open Research, 2022 Background Human nasal epithelial (HNE) cells can be sampled noninvasively and cultured to provide a model of the airway epithelium that reflects cystic fibrosis (CF) pathophysiology.
Timothy E. Corcoran +12 more
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FRAP analysis: accounting for bleaching during image capture. [PDF]
PLoS ONE, 2012 The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a ...
Jun Wu +3 more
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Defining the Diffusion in Model Membranes Using Line Fluorescence Recovery after Photobleaching [PDF]
Membranes, 2020 In this study, we explore the use of line FRAP to detect diffusion in synthetic lipid membranes. The study of the dynamics of these membrane lipids can, however, be challenging.
Jakob L. Kure +4 more
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Analysis of Diffusion of Ras2 in Saccharomyces cerevisiae Using Fluorescence Recovery after Photobleaching [PDF]
, 2010 Binding, lateral diffusion and exchange are fundamental dynamic processes involved in protein association with cellular membranes. In this study, we developed numerical simulations of lateral diffusion and exchange of fluorophores in membranes with ...
Daniel A Beard +10 more
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