Determining vitreous viscosity using fluorescence recovery after photobleaching. [PDF]
PLoS ONE, 2022 PurposeVitreous humor is a complex biofluid whose composition determines its structure and function. Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
Nishanthan Srikantha +6 more
doaj +10 more sources
Analysis of Exosome Transfer in Mammalian Cells by Fluorescence Recovery after Photobleaching [PDF]
Bio-Protocol, 2018 During the course of evolution, prokaryote and eukaryote cells have developed elegant and to some extent analogous strategies to communicate with each other and to adapt to their surrounding environment.
Hernán González-King +9 more
doaj +8 more sources
Monitoring condensate dynamics in S. cerevisiae using fluorescence recovery after photobleaching [PDF]
STAR Protocols, 2022 Summary: This protocol describes the use of fluorescence recovery after photobleaching (FRAP) to investigate the dynamics of Matrin-3 (MATR3) condensates in live budding yeast.
Macy L. Sprunger, Meredith E. Jackrel
doaj +3 more sources
Nasal epithelial cell culture fluorescence recovery after photobleaching predicts cystic fibrosis therapeutic response [PDF]
ERJ Open Research, 2022 Background Human nasal epithelial (HNE) cells can be sampled noninvasively and cultured to provide a model of the airway epithelium that reflects cystic fibrosis (CF) pathophysiology.
Timothy E. Corcoran +12 more
doaj +4 more sources
Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells [PDF]
STAR Protocols, 2021 Summary: Single-molecule fluorescence recovery after photobleaching (smFRAP) is a newly developed technique that combines single-molecule super-resolution microscopy and traditional FRAP microscopy.
Mark Tingey, Yichen Li, Weidong Yang
doaj +3 more sources
Fluorescence recovery after photobleaching: analyses of cyanobacterial phycobilisomes reveal intrinsic fluorescence recovery. [PDF]
Mar Life Sci Technol, 2021 Fluorescence recovery after photobleaching (FRAP) has been used to study the dynamics of the cyanobacterial photosynthesis apparatus since 1997. Fluorescence recovery of cyanobacteria during FRAP was conventionally interpreted as a result of phycobilisome (PBS) diffusion on the surface of the thylakoid membrane.
Zhang N +6 more
europepmc +5 more sources
Investigating the effect of proteoglycan 4 on hyaluronan solution properties using confocal fluorescence recovery after photobleaching [PDF]
BMC Musculoskeletal Disorders, 2019 Background The objective of this study was to use confocal fluorescence recovery after photobleaching (FRAP) to examine the specific and dose-dependent effect of proteoglycan 4 (PRG4) on hyaluronan (HA) solutions of different molecular weight; and assess
Adam K. Bloom +4 more
doaj +4 more sources
Using Fluorescence Recovery After Photobleaching data to uncover filament dynamics. [PDF]
PLoS Computational Biology, 2022 Fluorescence Recovery After Photobleaching (FRAP) has been extensively used to understand molecular dynamics in cells. This technique when applied to soluble, globular molecules driven by diffusion is easily interpreted and well understood.
J C Dallon +5 more
doaj +2 more sources
The Utility of Fluorescence Recovery after Photobleaching (FRAP) to Study the Plasma Membrane [PDF]
Membranes, 2023 The plasma membrane of mammalian cells is involved in a wide variety of cellular processes, including, but not limited to, endocytosis and exocytosis, adhesion and migration, and signaling.
Charles A. Day, Minchul Kang
doaj +2 more sources
Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM [PDF]
Molecules, 2012 Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Gregor P. C. Drummen +2 more
doaj +4 more sources