Analysis of Exosome Transfer in Mammalian Cells by Fluorescence Recovery after Photobleaching [PDF]
Bio-Protocol, 2018 During the course of evolution, prokaryote and eukaryote cells have developed elegant and to some extent analogous strategies to communicate with each other and to adapt to their surrounding environment.
Hernán González-King +9 more
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Measurement of Junctional Protein Dynamics Using Fluorescence Recovery After Photobleaching (FRAP) [PDF]
Bio-Protocol, 2013 Fluorescence Recovery After Photobleaching (FRAP) (Lippincott-Schwartz et al., 2003; Reits and Neefjes, 2001) was employed to determine dynamic properties of proteins localized at the ephitelial zonula adherens (ZA) (Kovacs et al., 2011; Otani et al ...
Rashmi Priya, Guillermo Gomez
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Expanding the applicability of multiphoton fluorescence recovery after photobleaching by incorporating shear stress in laminar flow. [PDF]
J Biomed Opt, 2023 Elias TM, Brown EB, Brown EB.
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Long-term molecular turnover of actin stress fibers revealed by advection-reaction analysis in fluorescence recovery after photobleaching. [PDF]
PLoS ONE, 2022 Fluorescence recovery after photobleaching (FRAP) is a versatile technique to evaluate the intracellular molecular exchange called turnover. Mechanochemical models of FRAP typically consider the molecular diffusion and chemical reaction that ...
Takumi Saito +2 more
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Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells [PDF]
STAR Protocols, 2021 Summary: Single-molecule fluorescence recovery after photobleaching (smFRAP) is a newly developed technique that combines single-molecule super-resolution microscopy and traditional FRAP microscopy.
Mark Tingey, Yichen Li, Weidong Yang
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A fluorescence recovery after photobleaching protocol to measure surface diffusion of DAGLα in primary cultured cortical mouse neurons. [PDF]
STAR Protoc, 2022 Yoon S, Penzes P.
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Investigating the effect of proteoglycan 4 on hyaluronan solution properties using confocal fluorescence recovery after photobleaching [PDF]
BMC Musculoskeletal Disorders, 2019 Background The objective of this study was to use confocal fluorescence recovery after photobleaching (FRAP) to examine the specific and dose-dependent effect of proteoglycan 4 (PRG4) on hyaluronan (HA) solutions of different molecular weight; and assess
Adam K. Bloom +4 more
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X-Ray Photon Correlation Spectroscopy, Microscopy, and Fluorescence Recovery After Photobleaching to Study Phase Separation and Liquid-to-Solid Transition of Prion Protein Condensates. [PDF]
Bio Protocdo Amaral MJ +5 more
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Assessing cellular efficacy of bromodomain inhibitors using fluorescence recovery after photobleaching. [PDF]
Epigenetics Chromatin, 2014 BACKGROUND: Acetylation of lysine residues in histone tails plays an important role in the regulation of gene transcription. Bromdomains are the readers of acetylated histone marks, and, consequently, bromodomain-containing proteins have a variety of ...
Philpott M +9 more
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Parameter identifiability in PDE models of fluorescence recovery after photobleaching [PDF]
Bulletin of Mathematical Biology, 2023 Identifying unique parameters for mathematical models describing biological data can be challenging and often impossible. Parameter identifiability for partial differential equations models in cell biology is especially difficult given that many established \textit{in vivo} measurements of protein dynamics average out the spatial dimensions.
Maria-Veronica Ciocanel +6 more
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