Results 11 to 20 of about 40,790 (312)
FRAP analysis: accounting for bleaching during image capture. [PDF]
PLoS ONE, 2012 The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a ...
Jun Wu +3 more
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Fluorescence recovery after photobleaching (FRAP) v1 [PDF]
, 2022 This protocol details methods of the FRAP analysis of LRRK2-induced liposome tubules in vitro
Xinbo Wang
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Parameter identifiability in PDE models of fluorescence recovery after photobleaching [PDF]
Bulletin of Mathematical Biology, 2023 Identifying unique parameters for mathematical models describing biological data can be challenging and often impossible. Parameter identifiability for partial differential equations models in cell biology is especially difficult given that many established \textit{in vivo} measurements of protein dynamics average out the spatial dimensions.
Maria-Veronica Ciocanel +6 more
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Defining the Diffusion in Model Membranes Using Line Fluorescence Recovery after Photobleaching [PDF]
Membranes, 2020 In this study, we explore the use of line FRAP to detect diffusion in synthetic lipid membranes. The study of the dynamics of these membrane lipids can, however, be challenging.
Jakob L. Kure +4 more
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Protein Synthesis Rate Assessment by Fluorescence Recovery after Photobleaching (FRAP) [PDF]
Bio-Protocol, 2017 Currently available biochemical methods cannot be applied to monitor protein synthesis in specific cells or tissues, in live specimens. Here, we describe a non-invasive method for monitoring protein synthesis in single cells or tissues with intrinsically
Nikos Kourtis, Nektarios Tavernarakis
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Journal of Innovative Optical Health Sciences, 2021 Fluorescence recovery after photobleaching (FRAP) and single particle tracking (SPT) techniques determine the diffusion coefficient from average diffusive motion of high-concentration molecules and from trajectories of low-concentration single molecules,
Donghee Lee +2 more
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Long-term molecular turnover of actin stress fibers revealed by advection-reaction analysis in fluorescence recovery after photobleaching. [PDF]
PLoS ONE, 2022 Fluorescence recovery after photobleaching (FRAP) is a versatile technique to evaluate the intracellular molecular exchange called turnover. Mechanochemical models of FRAP typically consider the molecular diffusion and chemical reaction that ...
Takumi Saito +2 more
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Single- and two-photon fluorescence recovery after photobleaching. [PDF]
Cold Spring Harb Protoc, 2015 Fluorescence recovery after photobleaching (FRAP) is a microscopy technique for measuring the kinetics of fluorescently labeled molecules and can be applied both in vitro and in vivo for two- and three-dimensional systems. This introduction discusses the three basic FRAP methods: traditional FRAP, multiphoton FRAP (MPFRAP), and FRAP with spatial ...
Sullivan KD, Majewska AK, Brown EB.
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Analysis of Active Transport by Fluorescence Recovery after Photobleaching. [PDF]
Biophys J, 2017 Fluorescence recovery after photobleaching (FRAP) is a well-established experimental technique to study binding and diffusion of molecules in cells. Although a large number of analytical and numerical models have been developed to extract binding and diffusion rates from FRAP recovery curves, active transport of molecules is typically not included in ...
Ciocanel MV +4 more
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Assessing cellular efficacy of bromodomain inhibitors using fluorescence recovery after photobleaching. [PDF]
Epigenetics Chromatin, 2014 BACKGROUND: Acetylation of lysine residues in histone tails plays an important role in the regulation of gene transcription. Bromdomains are the readers of acetylated histone marks, and, consequently, bromodomain-containing proteins have a variety of ...
Philpott M +9 more
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