Multiphoton fluorescence recovery after photobleaching in bounded systems. [PDF]
Multiphoton fluorescence recovery after photobleaching (MP-FRAP) is a laser microscopy technique used to measure diffusion coefficients of macromolecules in biological systems. The three-dimensional resolution and superior depth penetration within scattering samples offered by MP-FRAP make it an important tool for investigating both in vitro and in ...
Sullivan KD, Brown EB.
europepmc +4 more sources
DeepFRAP: Fast fluorescence recovery after photobleaching data analysis using deep neural networks. [PDF]
Wåhlstrand Skärström V +3 more
europepmc +3 more sources
A reaction–subdiffusion model of fluorescence recovery after photobleaching (FRAP) [PDF]
Anomalous diffusion, in particular subdiffusion, is frequently invoked as a mechanism of motion in dense biological media, and may have a significant impact on the kinetics of binding/unbinding events at the cellular level. In this work we incorporate anomalous diffusion in a previously developed model for FRAP experiments.
S. B. Yuste, E. Abad, Katja Lindenberg
openalex +4 more sources
Recent Advances in Fluorescence Recovery after Photobleaching for Decoupling Transport and Kinetics of Biomacromolecules in Cellular Physiology. [PDF]
Cai N +5 more
europepmc +3 more sources
Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching. [PDF]
Taylor NO +3 more
europepmc +3 more sources
Lateral mobility in membranes as detected by fluorescence recovery after photobleaching
The evaluation of lateral diffusion coefficients of membrane components by the technique of fluorescence recovery after photobleaching (FRAP) is often complicated by uncertainties in the values of the intensities F(O), immediately after bleaching, and F(infinity), after full recovery.
Juan Yguerabide +2 more
openalex +3 more sources
Probing Native CB<sub>2</sub> Receptor Mobility in Plasma Membranes of Living Cells by Fluorescence Recovery After Photobleaching. [PDF]
Ciaramellano F +9 more
europepmc +3 more sources
Measurement of three-dimensional anisotropic diffusion by multiphoton fluorescence recovery after photobleaching. [PDF]
Shi C, Cisewski SE, Bell PD, Yao H.
europepmc +2 more sources
UV Light Induced Fluorescence Recovery of GFP After Photobleaching in Microscopy Imaging
Fluorescence microscopy has become one of the most important tools for biologists to visualize and study organelles and molecules in a cell. Fluorescent markers are used to visualize specific molecules.
Griša G. Prinčič +4 more
doaj +1 more source
Photobleaching and Fluorescence Recovery of RPE Bisretinoids. [PDF]
The autofluorescence of the retina that originates primarily from lipofuscin fluorophores in retinal pigment epithelial cells, is observed to undergo photobleaching during the acquisition of fundus autofluorescence images.
Zhao Liu +3 more
doaj +1 more source

