Fluorescence recovery after photobleaching: analyses of cyanobacterial phycobilisomes reveal intrinsic fluorescence recovery. [PDF]
Mar Life Sci Technol, 2021 Fluorescence recovery after photobleaching (FRAP) has been used to study the dynamics of the cyanobacterial photosynthesis apparatus since 1997. Fluorescence recovery of cyanobacteria during FRAP was conventionally interpreted as a result of phycobilisome (PBS) diffusion on the surface of the thylakoid membrane.
Zhang N +6 more
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EGF Receptor Stalls upon Activation as Evidenced by Complementary Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching Measurements. [PDF]
Int J Mol Sci, 2019 Vámosi G +5 more
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Determination of diffusion coefficients in live cells using fluorescence recovery after photobleaching with wide-field fluorescence microscopy. [PDF]
Biophys Physicobiol, 2018 Kitamura A, Kinjo M.
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Quantitative Nanometrology of Binary Particle Systems Using Fluorescence Recovery after Photobleaching: Application to Colloidal Silica. [PDF]
LangmuirDoveiko D +6 more
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UV Light Induced Fluorescence Recovery of GFP After Photobleaching in Microscopy Imaging
Image Analysis and Stereology, 2022 Fluorescence microscopy has become one of the most important tools for biologists to visualize and study organelles and molecules in a cell. Fluorescent markers are used to visualize specific molecules.
Griša G. Prinčič +4 more
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Erythrocytes membrane fluidity changes induced by adenylyl cyclase cascade activation: study using fluorescence recovery after photobleaching. [PDF]
Eur Biophys JSemenov AN +5 more
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Single- and two-photon fluorescence recovery after photobleaching. [PDF]
Cold Spring Harb Protoc, 2015 Fluorescence recovery after photobleaching (FRAP) is a microscopy technique for measuring the kinetics of fluorescently labeled molecules and can be applied both in vitro and in vivo for two- and three-dimensional systems. This introduction discusses the three basic FRAP methods: traditional FRAP, multiphoton FRAP (MPFRAP), and FRAP with spatial ...
Sullivan KD, Majewska AK, Brown EB.
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Photobleaching and Fluorescence Recovery of RPE Bisretinoids. [PDF]
PLoS ONE, 2015 The autofluorescence of the retina that originates primarily from lipofuscin fluorophores in retinal pigment epithelial cells, is observed to undergo photobleaching during the acquisition of fundus autofluorescence images.
Zhao Liu +3 more
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A reaction–subdiffusion model of fluorescence recovery after photobleaching (FRAP) [PDF]
Journal of Statistical Mechanics: Theory and Experiment, 2014 Anomalous diffusion, in particular subdiffusion, is frequently invoked as a mechanism of motion in dense biological media, and may have a significant impact on the kinetics of binding/unbinding events at the cellular level. In this work we incorporate anomalous diffusion in a previously developed model for FRAP experiments.
S. B. Yuste, E. Abad, Katja Lindenberg
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Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching [PDF]
Journal of Visualized Experiments, 2010 Multi-fluorescence recovery after photobleaching is a microscopy technique used to measure the diffusion coefficient (or analogous transport parameters) of macromolecules, and can be applied to both in vitro and in vivo biological systems. Multi-fluorescence recovery after photobleaching is performed by photobleaching a region of interest within a ...
Kelley D. Sullivan, Edward B. Brown
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