Transport between im/mobile fractions shapes the speed and profile of cargo distribution in neurons
Biophysical Reports, 2022 Neuronal function requires continuous distribution of ion channels and other proteins throughout large cell morphologies. Protein distribution is complicated by immobilization of freely diffusing subunits such as on lipid rafts or in postsynaptic ...
Adriano A. Bellotti +3 more
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Analysis of Binding Reactions by Fluorescence Recovery after Photobleaching [PDF]
Biophysical Journal, 2004 Fluorescence recovery after photobleaching (FRAP) is now widely used to investigate binding interactions in live cells. Although various idealized solutions have been identified for the reaction-diffusion equations that govern FRAP, there has been no comprehensive analysis or systematic approach to serve as a guide for extracting binding information ...
Sprague, Brian L. +3 more
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Effects of Organelle Shape on Fluorescence Recovery after Photobleaching [PDF]
Biophysical Journal, 2005 The determination of diffusion coefficients from fluorescence recovery data is often complicated by geometric constraints imposed by the complex shapes of intracellular compartments. To address this issue, diffusion of proteins in the lumen of the endoplasmic reticulum (ER) is studied using cell biological and computational methods.
Sbalzarini, Ivo F. +3 more
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A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion
Nature Communications, 2021 It is challenging to quantify anisotropic diffusion in biological systems. Here the authors report light-sheet imaging-based Fourier transform fluorescence recovery after photobleaching (LiFT-FRAP) to noninvasively determine 3D diffusion tensors of ...
Peng Chen +9 more
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Fluorescence recovery after photobleaching on the confocal laser-scanning microscope: generalized model without restriction on the size of the photobleached disk [PDF]
, 2011 Fluorescence recovery after photobleaching (FRAP) carried out on a confocal laser-scanning microscope (CLSM) performs well for photobleached disks that are large compared to the resolution of the bleaching beam.
Ameloot, Marcel +6 more
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eLife, 2020 Wnt3 proteins are lipidated and glycosylated signaling molecules that play an important role in zebrafish neural patterning and brain development. However, the transport mechanism of lipid-modified Wnts through the hydrophilic extracellular environment ...
Sapthaswaran Veerapathiran +6 more
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eLife, 2022 Synapses maintain both action potential-evoked and spontaneous neurotransmitter release; however, organization of these two forms of release within an individual synapse remains unclear.
Camille S Wang +3 more
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Fluorescence Recovery After Photobleaching (FRAP) in the Fission Yeast Nucleus
Bio-Protocol, 2013 We use fluorescence recovery after photobleaching (FRAP) to calculate the diffusion coefficient of GFP in the nucleoplasm of fission yeast. The FRAP method can be generally used to measure the mobility of proteins inside the cell or its organelles.
Petrina Delivani +3 more
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Fluorescence Recovery after Photobleaching: The Case of Anomalous Diffusion [PDF]
Biophysical Journal, 2008 The method of FRAP (fluorescence recovery after photobleaching), which has been broadly used to measure lateral mobility of fluorescent-labeled molecules in cell membranes, is formulated here in terms of continuous time random walks (CTRWs), which offer both analytical expressions and a scheme for numerical simulations.
Lubelski, Ariel, Klafter, Joseph
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Some Secrets of Fluorescent Proteins: Distinct Bleaching in Various Mounting Fluids and Photoactivation of cyan fluorescent proteins at YFP-Excitation [PDF]
, 2011 Background The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking specific proteins or cells
AF Fradkov +33 more
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