Results 31 to 40 of about 41,336 (313)
Analysis of Active Transport by Fluorescence Recovery after Photobleaching. [PDF]
Biophys J, 2017 Fluorescence recovery after photobleaching (FRAP) is a well-established experimental technique to study binding and diffusion of molecules in cells. Although a large number of analytical and numerical models have been developed to extract binding and diffusion rates from FRAP recovery curves, active transport of molecules is typically not included in ...
Ciocanel MV +4 more
europepmc +4 more sources
Transport between im/mobile fractions shapes the speed and profile of cargo distribution in neurons
Biophysical Reports, 2022 Neuronal function requires continuous distribution of ion channels and other proteins throughout large cell morphologies. Protein distribution is complicated by immobilization of freely diffusing subunits such as on lipid rafts or in postsynaptic ...
Adriano A. Bellotti +3 more
doaj +1 more source
A noninvasive fluorescence imaging-based platform measures 3D anisotropic extracellular diffusion
Nature Communications, 2021 It is challenging to quantify anisotropic diffusion in biological systems. Here the authors report light-sheet imaging-based Fourier transform fluorescence recovery after photobleaching (LiFT-FRAP) to noninvasively determine 3D diffusion tensors of ...
Peng Chen +9 more
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Journal of Innovative Optical Health Sciences, 2021 Fluorescence recovery after photobleaching (FRAP) and single particle tracking (SPT) techniques determine the diffusion coefficient from average diffusive motion of high-concentration molecules and from trajectories of low-concentration single molecules,
Donghee Lee +2 more
doaj +1 more source
Multiphoton fluorescence recovery after photobleaching in bounded systems. [PDF]
Phys Rev E Stat Nonlin Soft Matter Phys, 2011 Multiphoton fluorescence recovery after photobleaching (MP-FRAP) is a laser microscopy technique used to measure diffusion coefficients of macromolecules in biological systems. The three-dimensional resolution and superior depth penetration within scattering samples offered by MP-FRAP make it an important tool for investigating both in vitro and in ...
Sullivan KD, Brown EB.
europepmc +4 more sources
Rotational dynamics of colloidal spheres probed with fluorescence recovery after photobleaching [PDF]
The Journal of Chemical Physics, 2004 We report a polarized fluorescence recovery after photobleaching (pFRAP) method to measure the rotational dynamics of fluorescent colloids over a wide dynamic range. The method is based on the polarization anisotropy in the fluorescence intensity, generated by bleaching of fluorescently labeled particles with an intense pulse of linearly polarized ...
M. P. Lettinga +4 more
openalex +7 more sources
Fluorescence recovery after photobleaching on the confocal laser-scanning microscope: generalized model without restriction on the size of the photobleached disk [PDF]
, 2011 Fluorescence recovery after photobleaching (FRAP) carried out on a confocal laser-scanning microscope (CLSM) performs well for photobleached disks that are large compared to the resolution of the bleaching beam.
Ameloot, Marcel +6 more
core +2 more sources
Fluorescence recovery after photobleaching: direct measurement of diffusion anisotropy [PDF]
Biomechanics and Modeling in Mechanobiology, 2020 Fluorescence recovery after photobleaching (FRAP) is a widely used technique for studying diffusion in biological tissues. Most of the existing approaches for the analysis of FRAP experiments assume isotropic diffusion, while only a few account for anisotropic diffusion. In fibrous tissues, such as articular cartilage, tendons and ligaments, diffusion,
Hashlamoun, Kotaybah +5 more
openaire +3 more sources
eLife, 2020 Wnt3 proteins are lipidated and glycosylated signaling molecules that play an important role in zebrafish neural patterning and brain development. However, the transport mechanism of lipid-modified Wnts through the hydrophilic extracellular environment ...
Sapthaswaran Veerapathiran +6 more
doaj +1 more source
eLife, 2022 Synapses maintain both action potential-evoked and spontaneous neurotransmitter release; however, organization of these two forms of release within an individual synapse remains unclear.
Camille S Wang +3 more
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