Fluorescence Recovery After Photobleaching (FRAP) in the Fission Yeast Nucleus
Bio-Protocol, 2013 We use fluorescence recovery after photobleaching (FRAP) to calculate the diffusion coefficient of GFP in the nucleoplasm of fission yeast. The FRAP method can be generally used to measure the mobility of proteins inside the cell or its organelles.
Petrina Delivani +3 more
doaj +1 more source
Primary cultures of chick osteocytes retain functional gap junctions between osteocytes and between osteocytes and osteoblasts [PDF]
, 2008 The inaccessibility of osteocytes due to their embedment in the calcified bone matrix in vivo has precluded direct demonstration that osteocytes use gap junctions as a means of intercellular communication.
Ishihara, Yoshihito +6 more
core +1 more source
Sensors, 2010 This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly focusing on membrane protein studies.
Gamal Rayan +4 more
doaj +1 more source
Diffusion studies through fluorescence recovery after photobleaching in hydrated polyamides
Polymer Engineering & Science, 2010 Abstract The effect of hydrogen bonding on the diffusivities of two almost identical fluorescent probes through polyamides was examined using fluorescence recovery after photobleaching (FRAP). The two molecules have the same geometry and a negligible difference in their molecular weight, the main difference confined is one ...
N. B. Hatzigrigoriou +4 more
openalex +4 more sources
Viruses, 2021 HIV-1 infection is enhanced by cell–cell adhesions between infected and uninfected T cells called virological synapses (VS). VS are initiated by the interactions of cell-surface HIV-1 envelope glycoprotein (Env) and CD4 on target cells and act as sites ...
Lili Wang +5 more
doaj +1 more source
Age-dependency of molecular diffusion in the human anterior lens capsule assessed using fluorescence recovery after photobleaching. [PDF]
Mol Vis, 2019 Sueiras VM +7 more
europepmc +2 more sources
Some Secrets of Fluorescent Proteins: Distinct Bleaching in Various Mounting Fluids and Photoactivation of cyan fluorescent proteins at YFP-Excitation [PDF]
, 2011 Background The use of spectrally distinct variants of green fluorescent protein (GFP) such as cyan or yellow mutants (CFP and YFP, respectively) is very common in all different fields of life sciences, e.g. for marking specific proteins or cells
AF Fradkov +33 more
core +4 more sources
Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress [PDF]
, 2018 Mechanical stimuli play a key role in many cell functions such as proliferation, differentiation and migration. In the mammary gland, mechanical signals such as the distension of mammary epithelial cells due to udder filling are proposed to be directly ...
Bianchi, Micaela +5 more
core +1 more source
Probing the Molecular Mechanism of Viscoelastic Relaxation in Transient Networks
Gels, 2023 Hydrogels, which have polymer networks through supramolecular and reversible interactions, exhibit various mechanical responsibilities to its surroundings.
Shota Michida +2 more
doaj +1 more source
Length-dependent disassembly maintains four different flagellar lengths in Giardia. [PDF]
, 2019 With eight flagella of four different lengths, the parasitic protist Giardia is an ideal model to evaluate flagellar assembly and length regulation. To determine how four different flagellar lengths are maintained, we used live-cell quantitative imaging ...
Aurrecoechea +69 more
core +2 more sources