Results 41 to 50 of about 40,790 (312)

Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules

open access: yesSensors, 2010
This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly focusing on membrane protein studies.
Gamal Rayan   +4 more
doaj   +1 more source

Measurement of Junctional Protein Dynamics Using Fluorescence Recovery After Photobleaching (FRAP)

open access: yesBio-Protocol, 2013
Fluorescence Recovery After Photobleaching (FRAP) (Lippincott-Schwartz et al., 2003; Reits and Neefjes, 2001) was employed to determine dynamic properties of proteins localized at the ephitelial zonula adherens (ZA) (Kovacs et al., 2011; Otani et al ...
Rashmi Priya, Guillermo Gomez
doaj   +1 more source

A Replication-Competent HIV Clone Carrying GFP-Env Reveals Rapid Env Recycling at the HIV-1 T Cell Virological Synapse

open access: yesViruses, 2021
HIV-1 infection is enhanced by cell–cell adhesions between infected and uninfected T cells called virological synapses (VS). VS are initiated by the interactions of cell-surface HIV-1 envelope glycoprotein (Env) and CD4 on target cells and act as sites ...
Lili Wang   +5 more
doaj   +1 more source

Primary cultures of chick osteocytes retain functional gap junctions between osteocytes and between osteocytes and osteoblasts [PDF]

open access: yes, 2008
The inaccessibility of osteocytes due to their embedment in the calcified bone matrix in vivo has precluded direct demonstration that osteocytes use gap junctions as a means of intercellular communication.
Ishihara, Yoshihito   +6 more
core   +1 more source

A pharmacological cocktail for arresting actin dynamics in living cells. [PDF]

open access: yes, 2011
The actin cytoskeleton is regulated by factors that influence polymer assembly, disassembly, and network rearrangement. Drugs that inhibit these events have been used to test the role of actin dynamics in a wide range of cellular processes.
Peng, Grace E   +2 more
core   +2 more sources

Probing the Molecular Mechanism of Viscoelastic Relaxation in Transient Networks

open access: yesGels, 2023
Hydrogels, which have polymer networks through supramolecular and reversible interactions, exhibit various mechanical responsibilities to its surroundings.
Shota Michida   +2 more
doaj   +1 more source

Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology

open access: yesData in Brief, 2016
The presented data provides additional information about the assessment of affinity purified nicotinic acetylcholine receptor (nAChR) rich membrane solubilized with long chain (16 saturated carbons) lysophospholipid with glycerol headgroup (LFG-16).
Luis F. Padilla-Morales   +4 more
doaj   +1 more source

Modern fluorescent techniques to investigate the mechanisms of lymphocyte activation [PDF]

open access: yesThe Ukrainian Biochemical Journal, 2015
Fluorescent proteins are promising tools for studying intracellular signaling processes in lymphocytes. This brief review summarizes fluorescence-based imaging techniques developed in recent years and discusses new methodological advances, such as ...
G. A. Liubchenko   +2 more
doaj   +1 more source

Unbreakable codes in electrospun fibers : digitally encoded polymers to stop medicine counterfeiting [PDF]

open access: yes, 2010
Fluorescent polymer solutions can easily be electrospun into micrometer-sized fibers and subsequently encoded with long lasting digital codes by a photobleaching process.
Braeckmans, Kevin   +9 more
core   +2 more sources

Effects of solute size and tissue composition on molecular and macromolecular diffusivity in human knee cartilage

open access: yesOsteoarthritis and Cartilage Open, 2020
Summary: Objective: Articular cartilage is an avascular tissue. Accordingly, diffusivity represents a fundamental transport mechanism for nutrients and other molecular signals regulating its cell metabolism and maintenance of the extracellular matrix ...
Francesco Travascio   +2 more
doaj   +1 more source

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