Results 41 to 50 of about 7,556 (242)

Natural Killer Cells in Antibody Independent and Antibody Dependent HIV Control

Frontiers in Immunology, 2022
Infection with the human immunodeficiency virus (HIV), when left untreated, typically leads to disease progression towards acquired immunodeficiency syndrome.
Nicole F. Bernard, Nicole F. Bernard, Nicole F. Bernard, Nicole F. Bernard, Sanket Kant, Sanket Kant, Sanket Kant, Zahra Kiani, Zahra Kiani, Zahra Kiani, Cécile Tremblay, Cécile Tremblay, Franck P. Dupuy, Franck P. Dupuy   +13 more
doaj   +1 more source

Impact of LS Mutation on Pharmacokinetics of Preventive HIV Broadly Neutralizing Monoclonal Antibodies: A Cross-Protocol Analysis of 16 Clinical Trials in People without HIV

Pharmaceutics
Monoclonal antibodies are commonly engineered with an introduction of Met428Leu and Asn434Ser, known as the LS mutation, in the fragment crystallizable region to improve pharmacokinetic profiles.
Bryan T. Mayer, Lily Zhang, Allan C. deCamp, Chenchen Yu, Alicia Sato, Heather Angier, Kelly E. Seaton, Nicole Yates, Julie E. Ledgerwood, Kenneth Mayer, Marina Caskey, Michel Nussenzweig, Kathryn Stephenson, Boris Julg, Dan H. Barouch, Magdalena E. Sobieszczyk, Srilatha Edupuganti, Colleen F. Kelley, M. Juliana McElrath, Huub C. Gelderblom, Michael Pensiero, Adrian McDermott, Lucio Gama, Richard A. Koup, Peter B. Gilbert, Myron S. Cohen, Lawrence Corey, Ollivier Hyrien, Georgia D. Tomaras, Yunda Huang   +29 more
doaj   +1 more source

A plant-biotechnology approach for producing highly potent anti-HIV antibodies for antiretroviral therapy consideration

Journal of Genetic Engineering and Biotechnology, 2021
Despite a reduction in global HIV prevalence the development of a pipeline of new therapeutics or pre-exposure prophylaxis to control the HIV/AIDS epidemic are of high priority.
Advaita Acarya Singh, Priyen Pillay, Lusisizwe Kwezi, Tsepo Lebiletsa Tsekoa   +3 more
doaj   +1 more source

Differences in human IgG1 and IgG4 S228P monoclonal antibodies viscosity and self-interactions: Experimental assessment and computational predictions of domain interactions

mAbs, 2021
Human/humanized IgG4 antibodies have reduced effector function relative to IgG1 antibodies, which is desirable for certain therapeutic purposes. However, the developability and biophysical properties for IgG4 antibodies are not well understood. This work
Pin-Kuang Lai, Gaurav Ghag, Yao Yu, Veronica Juan, Laurence Fayadat-Dilman, Bernhardt L. Trout   +5 more
doaj   +1 more source

Factors Affecting the FcRn-Mediated Transplacental Transfer of Antibodies and Implications for Vaccination in Pregnancy. [PDF]

, 2017
At birth, neonates are particularly vulnerable to infection and transplacental transfer of immunoglobulin G (IgG) from mother to fetus provides crucial protection in the first weeks of life.
Abdiche, Abourawi, Abu-Raya, Amirthalingam, Amirthalingam, Antohe, Atwell, Bainbridge, Bakchoul, Baker, Baker, Basha, Bialas, Bondt, Brair, Brambell, Brambell, Brambell, Bruhns, Calderon, Calvert, Cavalcante, Chen, Chu, Cumberland, Dabrera, Dashivets, de Moraes-Pinto, de Souza, Dillon, Einarsdottir, Einarsdottir, Ellinger, Ellinger, Englund, Faucette, Faucette, Firan, Fowden, França, Fu, Gaither, Gall, Goldfarb, Griffiths, Hamilton, Hammes, Hanson, Hartter, Hashira, Heath, Heidl, Henkle, Heron, Holder, Holland, Hornby, Huhn, Jansen, Jauniaux, Jefferis, Jones, Junghans, Kennedy, Kibe, Kim, Kim, Kim, Kohler, Kristoffersen, Ladhani, Leach, Leitner, Li, Li, Lightle, Madhi, Maertens, Malek, Malek, Mammaro, Martin, Martins, Mathiesen, Mathiesen, Medesan, Mestas, Mimura, Mitchell, Mohanty, Montano, Moore, Myllynen, Neuber, Niewiesk, Ober, Ober, Oganesyan, Okoko, Okoko, Okoko, Okoko, Omata, Osendarp, Palmeira, Palmer, Poehling, Porter, Poulsen, Powner, Qiao, Raghavan, Raghavan, Reynolds, Roux, Sand, Saso, Schlaudecker, Schofield, Scott, Sgarbosa, Silveira Lessa, Simister, Simister, Simister, Stach, Stapleton, Stapleton, Syme, Tabata, Takizawa, Tapia, Thomann, Tzaban, van den Berg, van den Berg, Viall, Vidarsson, Vidarsson, Vogelzang, Vähäkangas, West, Williams, Winter, Wormald, Wypych, Zaman, Zhu   +147 more
core   +2 more sources

Modifications in FLAP's second cytosolic loop influence 5‐LOX interaction, inhibitor binding, and leukotriene formation

FEBS Letters, EarlyView.
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp, Katharina Rataj, Stefanie König, Marcia E. Newcomer, Oliver Werz, Ulrike Garscha   +5 more
wiley   +1 more source

Global and Local Conformation of Human IgG Antibody Variants Rationalizes Loss of Thermodynamic Stability. [PDF]

, 2015
Immunoglobulin G (IgG) monoclonal antibodies (mAbs) are a major class of medicines, with high specificity and affinity towards targets spanning many disease areas. The antibody Fc (fragment crystallizable) region is a vital component of existing antibody
Edgeworth, Matthew J, Higazi, Daniel R, Kippen, Alistair D, Lowe, David C, Phillips, Jonathan J, Scrivens, James H   +5 more
core   +1 more source

A nucleotide‐independent, pan‐RAS‐targeted DARPin elicits anti‐tumor activity in a multimodal manner

Molecular Oncology, EarlyView.
We report a Designed Ankyrin Repeat Protein that binds and inhibits RAS proteins, which serve as central cell signaling hubs and are essential for the progression of many cancers. Its unique feature is that it does not discriminate between different RAS isoforms or mutations and is capable of binding to RAS in both its active (GTP‐bound) and inactive ...
Jonas N. Kapp, Wouter P. R. Verdurmen, Jonas V. Schaefer, Kari Kopra, Gabriela Nagy‐Davidescu, Elodie Richard, Marie‐Julie Nokin, Patrick Ernst, Rastislav Tamaskovic, Martin Schwill, Ralph Degen, Claudia Scholl, David Santamaria, Andreas Plückthun   +13 more
wiley   +1 more source

Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast [PDF]

, 2012
We have constructed IgG1-Fc scaffolds with increased thermal stability by directed evolution and yeast surface display. As a basis a new selection strategy that allowed the application of yeast surface display for screening of stabilizing mutations in ...
Antes, Bernhard, Faissner, Maximilian, Hasenhindl, Christoph, Obinger, Christian, Rüker, Florian, Stadlmayr, Gerhard, Traxlmayr, Michael W.   +6 more
core   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source