Results 21 to 30 of about 291 (138)

Early Detection of Ovarian Cancer Using Cell-Free DNA Fragmentomes and Protein Biomarkers. [PDF]

Cancer Discov
Ovarian cancer is a leading cause of death for women worldwide in part due to ineffective screening methods. In this study, we used whole-genome cell-free DNA (cfDNA) fragmentome and protein biomarker (CA-125 and HE4) analyses to evaluate 591 women with ...
Medina JE, Annapragada AV, Lof P, Short S, Bartolomucci AL, Mathios D, Koul S, Niknafs N, Noë M, Foda ZH, Bruhm DC, Hruban C, Vulpescu NA, Jung E, Dua R, Canzoniero JV, Cristiano S, Adleff V, Symecko H, van den Broek D, Sokoll LJ, Baylin SB, Press MF, Slamon DJ, Konecny GE, Therkildsen C, Carvalho B, Meijer GA, Andersen CL, Domchek SM, Drapkin R, Scharpf RB, Phallen J, Lok CAR, Velculescu VE.   +34 more
europepmc   +2 more sources

Detecting Liver Cancer Using Cell-Free DNA Fragmentomes. [PDF]

Cancer Discov, 2023
Abstract Liver cancer is a major cause of cancer mortality worldwide. Screening individuals at high risk, including those with cirrhosis and viral hepatitis, provides an avenue for improved survival, but current screening methods are inadequate.
Foda ZH, Annapragada AV, Boyapati K, Bruhm DC, Vulpescu NA, Medina JE, Mathios D, Cristiano S, Niknafs N, Luu HT, Goggins MG, Anders RA, Sun J, Sun J, Meta SH, Thomas DL, Kirk GD, Adleff V, Phallen J, Scharpf RB, Kim AK, Velculescu VE.   +21 more
europepmc   +3 more sources

Cell-Free DNA Fragmentomics in Liquid Biopsy

Diagnostics, 2022
Cell-free DNA (cfDNA) in bodily fluids has rapidly transformed the development of noninvasive prenatal testing, cancer liquid biopsy, and transplantation monitoring. Plasma cfDNA consists of a mixture of molecules originating from various bodily tissues.
Spencer C. Ding, Y.M. Dennis Lo
openaire   +3 more sources

Emerging frontiers of cell-free DNA fragmentomics

Extracellular Vesicles and Circulating Nucleic Acids, 2022
Analysis of cell-free DNA (cfDNA) in the blood has shown promise for monitoring a variety of biological processes. Plasma cfDNA is a mixture comprising DNA molecules released from various bodily tissues, mediated by characteristic DNA fragmentations occurring during cell death.
Hu, Xi, Ding, Spencer C., Jiang, Peiyong
openaire   +2 more sources

At the dawn: cell-free DNA fragmentomics and gene regulation [PDF]

British Journal of Cancer, 2021
AbstractEpigenetic mechanisms play instrumental roles in gene regulation during embryonic development and disease progression. However, it is challenging to non-invasively monitor the dynamics of epigenomes and related gene regulation at inaccessible human tissues, such as tumours, fetuses and transplanted organs.
openaire   +2 more sources

Cell-Free DNA Fragmentomics: The Novel Promising Biomarker

International Journal of Molecular Sciences, 2023
Cell-free DNA molecules are released into the plasma via apoptotic or necrotic events and active release mechanisms, which carry the genetic and epigenetic information of its origin tissues. However, cfDNA is the mixture of various cell fragments, and the efficient enrichment of cfDNA fragments with diagnostic value remains a great challenge for ...
Ting Qi, Min Pan, Huajuan Shi, Liangying Wang, Yunfei Bai, Qinyu Ge   +5 more
openaire   +2 more sources

Cell-free epigenomes enhanced fragmentomics-based model for early detection of lung cancer. [PDF]

Clin Transl Med
Lung cancer‐related changes in fragmentomic features were unevenly distributed across the genome, showing significant enrichment in epigenetically regulated regions of specific genes. The cfDNA fragmentomics‐based model demonstrated superior detection capability, especially for early‐stage lung cancer.
Wang Y, Guo Q, Huang Z, Song L, Zhao F, Gu T, Feng Z, Wang H, Li B, Wang D, Zhou B, Guo C, Xu Y, Song Y, Zheng Z, Bing Z, Li H, Yu X, Fung KL, Xu H, Shi J, Chen M, Hong S, Jin H, Tong S, Zhu S, Zhu C, Song J, Liu J, Li S, Li H, Sun X, Liang N.   +32 more
europepmc   +2 more sources

Polyadenylation ligation‐mediated sequencing (PALM‐Seq) characterizes cell‐free coding and non‐coding RNAs in human biofluids

Clinical and Translational Medicine, Volume 12, Issue 7, July 2022., 2022
PALM‐Seq simultaneously characterizes the abundance and molecular signature of cell‐free coding and non‐coding RNAs. The profiles and end motifs of cf‐mRNA and cf‐lncRNA can distinguish biofluids. Seminal plasma tends to retain longer fragments than other biofluids.
Zhongzhen Liu, Taifu Wang, Xi Yang, Qing Zhou, Sujun Zhu, Juan Zeng, Haixiao Chen, Jinghua Sun, Liqiang Li, Jinjin Xu, Chunyu Geng, Xun Xu, Jian Wang, Huanming Yang, Shida Zhu, Fang Chen, Wen‐Jing Wang   +16 more
wiley   +1 more source

Circulating DNA fragmentomics and cancer screening

Cell Genomics, 2023
The high fragmentation of nuclear circulating DNA (cirDNA) relies on chromatin organization and protection or packaging within mononucleosomes, the smallest and the most stabilized structure in the bloodstream. The detection of differing size patterns, termed fragmentomics, exploits information about the nucleosomal packing of DNA.
openaire   +4 more sources

Epigenetic analysis of cell-free DNA by fragmentomic profiling

Proceedings of the National Academy of Sciences, 2022
Cell-free DNA (cfDNA) fragmentation patterns contain important molecular information linked to tissues of origin. We explored the possibility of using fragmentation patterns to predict cytosine-phosphate-guanine (CpG) methylation of cfDNA, obviating the use of bisulfite treatment and associated risks of DNA degradation.
Qing Zhou, Guannan Kang, Peiyong Jiang, Rong Qiao, W. K. Jacky Lam, Stephanie C. Y. Yu, Mary-Jane L. Ma, Lu Ji, Suk Hang Cheng, Wanxia Gai, Wenlei Peng, Huimin Shang, Rebecca W. Y. Chan, Stephen L. Chan, Grace L. H. Wong, Linda T. Hiraki, Stefano Volpi, Vincent W. S. Wong, John Wong, Rossa W. K. Chiu, K. C. Allen Chan, Y. M. Dennis Lo   +21 more
openaire   +2 more sources