A multi-center study for colorectal cancer early detection in high-risk disease patients using cell-free fragmentomics assay [PDF]
Cancer Cell InternationalBackground Early detection of colorectal cancer (CRC) is crucial for improving patient survival. This innovative multi-center study aims to develop a non-invasive blood-based assay using cell-free DNA (cfDNA) fragmentomics to differentiate CRC from ...
Jierong Chen +2 more
exaly +3 more sources
Longitudinal profiling of hormone receptor positive, HER2 negative metastatic breast cancer through droplet digital PCR-based circulating tumor DNA fragmentomics [PDF]
Translational OncologyBackground: In the context of hormone receptor positive, HER2 negative Metastatic breast cancer (MBC), CDK 4/6 inhibitors (CDK4/6i) combined with endocrine therapy represent the standard first-line treatment, improving Progression-Free Survival (PFS) and
Lorenzo Foffano +2 more
exaly +4 more sources
Circulating DNA fragmentomics and cancer screening
Cell Genomics, 2023 Summary: The high fragmentation of nuclear circulating DNA (cirDNA) relies on chromatin organization and protection or packaging within mononucleosomes, the smallest and the most stabilized structure in the bloodstream.
A.R. Thierry
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Cell-Free DNA Fragmentomics in Liquid Biopsy
Diagnostics, 2022 Cell-free DNA (cfDNA) in bodily fluids has rapidly transformed the development of noninvasive prenatal testing, cancer liquid biopsy, and transplantation monitoring. Plasma cfDNA consists of a mixture of molecules originating from various bodily tissues.
Spencer C Ding, Y M Dennis Lo
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Analysis of cfDNA fragmentomics metrics and commercial targeted sequencing panels [PDF]
Nature CommunicationsFragmentomics based analysis of cell-free DNA (cfDNA) has recently emerged as a method to infer epigenetic and transcriptional data. Many of these reports analyze whole genome sequencing (WGS) which is not readily available clinically.
Kyle T. Helzer +26 more
doaj +2 more sources
Fragmenting the future with FLARE: a comprehensive fragmentomics pipeline based on long-read nanopore sequencing [PDF]
Frontiers in GeneticsPurposeCell-free DNA (cfDNA) fragmentation patterns carry biological information beyond fragment length, revealing nuclease activity, chromatin organization, and tissue of origin.
Marica Ficorilli +14 more
doaj +2 more sources
DECIPHER-PRAD: an advanced fragmentomics-based cell-free DNA assay for prostate cancer early detection [PDF]
Cell Communication and SignalingEarly detection of prostate cancer is limited by the poor specificity of prostate-specific antigen (PSA)-based screening. Cell-free DNA (cfDNA) fragmentomics offers a promising non-invasive approach to improve screening accuracy and risk stratification ...
Shun Zhang +13 more
doaj +2 more sources
Liquid Biopsy in Early Screening of Cancers: Emerging Technologies and New Prospects [PDF]
BiomedicinesLiquid biopsy is moving beyond mutation-centric assays to multimodal frameworks that integrate cell-free DNA (cfDNA) signals with additional analytes such as circulating tumor cells (CTCs) and extracellular vesicles (EVs).
Hanyu Zhu +10 more
doaj +2 more sources
Cell-free nucleic acid fragmentomics: A non-invasive window into cellular epigenomes
Translational OncologyClinical genomic profiling of cell-free nucleic acids (e.g. cell-free DNA or cfDNA) from blood and other body fluids has ushered in a new era in non-invasive diagnostics and treatment monitoring strategies for health conditions and diseases such as ...
Subhajyoti De
exaly +3 more sources
Liquid biopsy epigenetics: establishing a molecular profile based on cell‐free DNA [PDF]
Molecular OncologyLiquid biopsies containing circulating tumor DNA (ctDNA) are important biomarkers across several forms of cancer. The detection of mutations in cell‐free DNA (cfDNA) indicates the presence of ctDNA.
Christoffer Trier Maansson +2 more
doaj +2 more sources