N1-methylpseudouridylation of mRNA causes +1 ribosomal frameshifting
Nature, 2023 AbstractIn vitro-transcribed (IVT) mRNAs are modalities that can combat human disease, exemplified by their use as vaccines for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). IVT mRNAs are transfected into target cells, where they are translated into recombinant protein, and the biological activity or immunogenicity of the encoded ...
Thomas E. Mulroney +19 more
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Structure of a Protozoan Virus from the Human Genitourinary Parasite Trichomonas vaginalis [PDF]
, 2013 The flagellated protozoan Trichomonas vaginalis is an obligate human genitourinary parasite and the most frequent cause of sexually transmitted disease worldwide. Most clinical isolates of T.
Asara, John M +10 more
core +1 more source
Mechanism of tRNA-mediated +1 ribosomal frameshifting [PDF]
Proceedings of the National Academy of Sciences, 2018 Significance Accurate gene expression is essential in all organisms. During protein synthesis, transfer RNAs (tRNAs) decode the genetic information contained in messenger RNA (mRNA) on the ribosome into amino acids using a defined 3-nt code. A fundamental question in biology is how the ribosome maintains this 3-nt code, or mRNA frame, during ...
Samuel, Hong +5 more
openaire +2 more sources
The phenotype of many independently isolated +1 frameshift suppressor mutants supports a pivotal role of the P-site in reading frame maintenance. [PDF]
PLoS ONE, 2013 The main features of translation are similar in all organisms on this planet and one important feature of it is the way the ribosome maintain the reading frame.
Gunilla Jäger +2 more
doaj +1 more source
Rational design of a synthetic mammalian riboswitch as a ligand-responsive -1 ribosomal frame-shifting stimulator [PDF]
, 2016 Metabolite-responsive RNA pseudoknots derived from prokaryotic riboswitches have been shown to stimulate −1 programmed ribosomal frameshifting (PRF), suggesting −1 PRF as a promising gene expression platform to extend riboswitch applications in higher ...
Chang, Kung-Yao, Lin, Ya-Hui, 張功耀
core +1 more source
Model of the pathway of −1 frameshifting: Long pausing
Biochemistry and Biophysics Reports, 2016 It has been characterized that the programmed ribosomal −1 frameshifting often occurs at the slippery sequence on the presence of a downstream mRNA pseudoknot. In some prokaryotic cases such as the dnaX gene of Escherichia coli, an additional stimulatory
Ping Xie
doaj +1 more source
An Equilibrium-Dependent Retroviral mRNA Switch Regulates Translational Recoding [PDF]
, 2011 Most retroviruses require translational recoding of a viral messenger RNA stop codon to maintain a precise ratio of structural (Gag) and enzymatic (Pol) proteins during virus assembly.
D'Souza, Victoria M +6 more
core +1 more source
Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation
Nature Communications, 2021 Translational frameshifting is a mechanism that expands the coding capabilities of mRNA. Here, structures of 70S ribosome complexes with GTPase elongation factor G (EF-G), a +1-frameshifting-prone mRNA and tRNAs reveal the cooperation between the ...
Gabriel Demo +7 more
doaj +1 more source
mBio, 2022 The constrained nature of viral genomes has allowed a translational sleight of hand known as −1 Programmed Ribosomal Frameshifting (−1 PRF) to flourish. Numerous studies have sought to tease apart the mechanisms and implications of −1PRF utilizing a few ...
Wesley D. Penn, Suchetana Mukhopadhyay
doaj +1 more source
Salt Effects on the Thermodynamics of a Frameshifting RNA Pseudoknot under Tension
, 2016 Because of the potential link between -1 programmed ribosomal frameshifting and response of a pseudoknot (PK) RNA to force, a number of single molecule pulling experiments have been performed on PKs to decipher the mechanism of programmed ribosomal ...
Denesyuk, Natalia A. +2 more
core +1 more source