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Bioluminescent determination of free fatty acids

Analytical Biochemistry, 1984
A simple, highly specific, and sensitive bioluminescent method for determination of free fatty acids in unextracted plasma or serum has been developed. The method is based on the activation of free fatty acids by acyl-CoA synthetase (EC 6.2.1.3). The pyrophosphate formed is used to phosphorylate fructose 6-phosphate in a reaction catalyzed by the ...
H, Kather, E, Wieland
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Myocardial tissue free fatty acids

Journal of Molecular and Cellular Cardiology, 1984
Myocardial ischaemia, whether produced by coronary artery ligation or by hypoxic low-flow perfusion of the isolated rat heart, has been shown to be associated with a significant depression in mitochondrial function, as well as an increase in tissue free fatty acid (FFA) levels.
Victor T., La Cock C., Lochner A.
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Plasma free fatty acids in mitochondrial fatty acid oxidation defects

Clinica Chimica Acta, 1997
Plasma free fatty acid profiles from patients suffering from various mitochondrial beta-oxidation deficiencies were analyzed by gas chromatography-mass spectrometry. cis-4-Decenoic acid (10:1n-6) in medium-chain acyl-CoA dehydrogenase deficiency and cis-5-tetradecenoic acid (14:1n-9) in very-long-chain and 3-hydroxy-long chain acyl-CoA dehydrogenase ...
G, Martínez   +7 more
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Relation of fatty acid structure to release and esterification of free fatty acids

American Journal of Physiology-Legacy Content, 1963
After incubation of rat adipose tissue with corticotrophin in buffer-albumin, the composition of the free fatty acids (FFA) in the tissue and in the medium was determined; the medium contained proportionately more myristic, palmitoleic, linoleic, and linolenic acids.
C H, Hollenberg, A, Angel
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The use of iodinated free fatty acids for assessing fatty acid metabolism

Journal of Nuclear Cardiology, 1994
Free fatty acid is a major substrate fuel for normal myocardium. Cardiovascular disease is frequently associated with impairment of fatty acid oxidation. Therefore assessment of fatty acid metabolism may be an important tool for the early detection of myocardial abnormalities and may provide insight into pathologic heart conditions.
N, Tamaki, M, Kawamoto
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Renal excretion of free fatty acids

Clinica Chimica Acta, 1971
Abstract Plasma and urine free fatty acids (FFA) were determined in the postabsorptive state in 25 subjects without renal disease. Urine contained 10.8 ± 2.7 μmoles/l FFA. The saturated fatty acids accounted for a higher percentage of the FFA in urine than in plasma. A correlation was observed between the urine/plasma ratios of FFA and creatinine and
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Free Fatty Acids in the Subretinal Fluid

Ophthalmologica, 2010
Free fatty acids (FFA) were determined in 50 samples of subretinal fluid (SRF) by the colorimetric method of Duncombe. The level of blood serum FFA was determined simultaneously. FFA in the SRF ranged between 0.01 and 1.0 µmol/ml in normal distribution with the maximum at low concentrations.
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Tolbutamide and Free Fatty Acids

The Journal of Clinical Endocrinology & Metabolism, 1969
R O, Weenink, R B, Smith
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