Results 21 to 30 of about 2,281 (190)

Multiscale comparative pathogenomic analysis of Vibrio anguillarum linking serotype diversity, genomic plasticity and pathogenicity [PDF]

Journal of Genetic Engineering and Biotechnology
Vibrio anguillarum is a major marine fish pathogen causing high mortality and potential zoonotic risks. Understanding its genomic diversity, virulence factors, and antibiotic resistance is crucial for aquaculture disease management.
Khandker Shahed, Ashim Chakma, Omar Hamza Bin Manjur, Sk Injamamul Islam   +3 more
doaj   +2 more sources

Evolution of mutations in the ftsI gene leading to amino acid substitutions in PBP3 in Haemophilus influenzae strains under the selective pressure of ampicillin and cefuroxime

International Journal of Medical Microbiology
Background: Aminopenicillins are recommended agents for non-invasive Haemophilus influenzae infections. One of the mechanisms of resistance to β-lactams is the alteration of the transpeptidase region of penicillin binding protein 3 (PBP3) which is caused
Vladislav Jakubu, Iveta Vrbova, Ibrahim Bitar, Marketa Cechova, Lucia Malisova, Helena Zemlickova   +5 more
doaj   +3 more sources

Purification of FtsW variants in a complex with FtsI/PBP3. [PDF]

, 2022
FtsW and FtsI/PBP3 were co-expressed in E. coli cells; FtsW contains a His-Tag and was used as bait to co-purify untagged FtsI/PBP3 on a HisTrap column. The samples were incubated with Bocillin FL to label FtsI and analyzed by SDS-PAGE.
Qingzhen Liao (11909921), Mohammed Terrak (494608), Shan Zhao (120517), Ying Li (38224), Eefjan Breukink (154126), Shishen Du (606940), Joe Lutkenhaus (606941), Yuanyuan Cui (276987), Han Gong (4764102), Adrien Boes (11909918)   +9 more
core   +1 more source

Quantification of co-localization of ZapA and FtsI or FtsN in cells overexpressing FtsW variants. [PDF]

, 2022
ZapA-mcherry rings and GFP-FtsI or GFP-FtsN rings displayed in Fig 6 and 7 were identified manually using ImageJ software. 50–200 ZapA-mcherry rings were examined for each strain and condition and the associated GFP-FtsI rings or GFP-FtsN rings were ...
Qingzhen Liao (11909921), Mohammed Terrak (494608), Shan Zhao (120517), Ying Li (38224), Eefjan Breukink (154126), Shishen Du (606940), Joe Lutkenhaus (606941), Yuanyuan Cui (276987), Han Gong (4764102), Adrien Boes (11909918)   +9 more
core   +1 more source

Overexpression of the dominant-negative FtsW mutants does not prevent localization of GFP-FtsI. [PDF]

, 2022
Overnight cultures of LYA4 (TB28, zapA-mcherry catfrt) carrying plasmid pLY114 (pBAD33, PBAD::gfp-linker-ftsI) were diluted 1:100 in fresh LB medium with antibiotics and cultured at 30°C.
Qingzhen Liao (11909921), Mohammed Terrak (494608), Shan Zhao (120517), Ying Li (38224), Eefjan Breukink (154126), Shishen Du (606940), Joe Lutkenhaus (606941), Yuanyuan Cui (276987), Han Gong (4764102), Adrien Boes (11909918)   +9 more
core   +1 more source

Control experiments for the purification of HisFtsW-FtsI/PBP3 shown in Fig 9. [PDF]

, 2022
Protein-protein interaction was assessed by co-expression and co-purification of His-tagged protein with untagged protein as indicated above each panel. The proteins are co-expressed in E.
Qingzhen Liao (11909921), Mohammed Terrak (494608), Shan Zhao (120517), Ying Li (38224), Eefjan Breukink (154126), Shishen Du (606940), Joe Lutkenhaus (606941), Yuanyuan Cui (276987), Han Gong (4764102), Adrien Boes (11909918)   +9 more
core   +1 more source

Conformational changes in the essential E. coli septal cell wall synthesis complex suggest an activation mechanism

Nature Communications, 2023
The bacterial divisome is a macromolecular machine composed of more than 30 proteins that controls cell wall constriction during division. Here, we present a model of the structure and dynamics of the core complex of the E.
Brooke M. Britton, Remy A. Yovanno, Sara F. Costa, Joshua McCausland, Albert Y. Lau, Jie Xiao, Zach Hensel   +6 more
doaj   +1 more source

Haemophilus influenzae carriage and antibiotic resistance profile in Belgian infants over a three-year period (2016–2018)

Frontiers in Microbiology, 2023
BackgroundNon-typeable Haemophilus influenzae has become increasingly important as a causative agent of invasive diseases following vaccination against H. influenzae type b.
Esra Ekinci, Laura Willen, Juan Pablo Rodriguez Ruiz, Kirsten Maertens, Liesbet Van Heirstraeten, Gabriela Serrano, Magali Wautier, Ariane Deplano, Herman Goossens, Pierre Van Damme, Philippe Beutels, Surbhi Malhotra-Kumar, Delphine Martiny, Heidi Theeten   +13 more
doaj   +1 more source

FtsI and FtsW Are Localized to the Septum in Escherichia coli [PDF]

Journal of Bacteriology, 1998
ABSTRACT The localization of FtsI (PBP3), a penicillin-binding protein specifically required for cell division in Escherichia coli , was investigated by immunofluorescence microscopy and found to localize to the septum. The localization of FtsI was not observed in ftsZ or
L, Wang, M K, Khattar, W D, Donachie, J, Lutkenhaus   +3 more
openaire   +2 more sources

Cefuroxime resistance in non-beta-lactamase Haemophilus influenzae is linked to mutations in ftsI [PDF]

Journal of Antimicrobial Chemotherapy, 2003
The penicillin binding protein (PBP) genes dacA, dacB and ftsI from 14 cefuroxime-resistant (CXM(R)) isolates and three clinical isolates with low CXM MIC for non-beta-lactamase-producing Haemophilus influenzae type b were molecularly characterized. One strain, 5788, was used to transform H.
K, Straker, M, Wootton, A M, Simm, P M, Bennett, A P, MacGowan, T R, Walsh   +5 more
openaire   +2 more sources