Diversity of Fungal DNA Methyltransferases and Their Association With DNA Methylation Patterns [PDF]
Frontiers in Microbiology, 2021 DNA methyltransferases (DNMTs) are a group of proteins that catalyze DNA methylation by transferring a methyl group to DNA. The genetic variation in DNMTs results in differential DNA methylation patterns associated with various biological processes.
Yu-Shin Nai +6 more
doaj +5 more sources
Comparison of methods for isolating fungal DNA [PDF]
Practical Laboratory Medicine, 2021 Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus Mucorales with special regard to the amount and purity of the DNA acquired. The acquired DNA was then amplified by specific real-time PCR.
Ilaria Galliano +7 more
doaj +5 more sources
Influence of DNA extraction kits on freshwater fungal DNA metabarcoding. [PDF]
PeerJ, 2022 AbstractBackgroundEnvironmental DNA (eDNA) metabarcoding is a common technique for efficient biodiversity monitoring, especially of microbes. Recently, the usefulness of aquatic eDNA in monitoring the diversity of both terrestrial and aquatic fungi has been suggested.
Matsuoka S, Sugiyama Y, Nagano M, Doi H.
europepmc +5 more sources
Optimizing fungal DNA extraction and purification for Oxford Nanopore untargeted shotgun metagenomic sequencing from simulated hemoculture specimens [PDF]
mSystemsLong-read metagenomics provides a promising alternative approach to fungal identification, circumventing methodological biases, associated with DNA amplification, which is a prerequisite for DNA barcoding/metabarcoding based on the primary fungal DNA ...
Nattapong Langsiri +8 more
doaj +2 more sources
Optimization of DNA extraction from human urinary samples for mycobiome community profiling. [PDF]
PLoS ONE, 2019 IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an
A Lenore Ackerman +8 more
doaj +4 more sources
Unraveling the ancient fungal DNA from the Iceman gut. [PDF]
BMC GenomicsAbstractHere, we explore the possible ancient fungal species in the gut of Ötzi, the Iceman, a naturally mummified human found in the Tyrolean Alps (border between Italy and Austria). While ancient DNA (aDNA) has been extensively used to study human, animal, and plant evolution, this research focuses on ancient microbial diversity, specifically fungi ...
Oskolkov N +8 more
europepmc +5 more sources
Comparison of methods for isolating fungal DNA [PDF]
Czech Journal of Food Sciences, 2011 In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as most detection techniques require DNA amplification ...
Petra MOŤKOVÁ, Jarmila VYTŘASOVÁ
doaj +2 more sources
Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification [PDF]
Open Life SciencesGenomic DNA (gDNA) extraction is an important step in many molecular studies of fungal biology, and it is necessary to evaluate the efficiency, cost-effectiveness, and efficacy of different extraction methods to ensure successful amplification of the ...
Dar Gulam Jeelani +5 more
doaj +2 more sources
Uncovering unseen fungal diversity from plant DNA banks [PDF]
PeerJ, 2017 Throughout the world DNA banks are used as storage repositories for genetic diversity of organisms ranging from plants to insects to mammals. Designed to preserve the genetic information for organisms of interest, these banks also indirectly preserve ...
Erin M. Datlof +7 more
doaj +4 more sources
Dye-based qPCR assays for the separate quantification of total bacterial, fungal, arthropod, and plant DNA. [PDF]
PLoS ONECharacterization of bacterial, fungal, arthropod, and plant communities from bulk environmental samples can enhance our understanding of the biodiversity on Earth and aid in biomonitoring of various biomes.
Teresa M Tiedge +2 more
doaj +2 more sources