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Diversity of Fungal DNA Methyltransferases and Their Association With DNA Methylation Patterns [PDF]

open access: yesFrontiers in Microbiology, 2021
DNA methyltransferases (DNMTs) are a group of proteins that catalyze DNA methylation by transferring a methyl group to DNA. The genetic variation in DNMTs results in differential DNA methylation patterns associated with various biological processes.
Yu-Shin Nai   +6 more
doaj   +5 more sources

Comparison of methods for isolating fungal DNA [PDF]

open access: yesPractical Laboratory Medicine, 2021
Objectives: The main aim of this work was to compare the methods of DNA isolation in the moulds of genus Mucorales with special regard to the amount and purity of the DNA acquired. The acquired DNA was then amplified by specific real-time PCR.
Ilaria Galliano   +7 more
doaj   +5 more sources

Optimizing fungal DNA extraction and purification for Oxford Nanopore untargeted shotgun metagenomic sequencing from simulated hemoculture specimens [PDF]

open access: yesmSystems
Long-read metagenomics provides a promising alternative approach to fungal identification, circumventing methodological biases, associated with DNA amplification, which is a prerequisite for DNA barcoding/metabarcoding based on the primary fungal DNA ...
Nattapong Langsiri   +8 more
doaj   +2 more sources

The Impact of DNA Extraction Methods on Stool Bacterial and Fungal Microbiota Community Recovery [PDF]

open access: goldFrontiers in Microbiology, 2019
Our understanding of human gut microbiota in health and disease depends on accurate and reproducible microbial data acquisition. The critical step in this process is to apply an appropriate methodology to extract microbial DNA, since biases introduced ...
Kristýna Fiedorová   +12 more
doaj   +2 more sources

Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification [PDF]

open access: yesOpen Life Sciences
Genomic DNA (gDNA) extraction is an important step in many molecular studies of fungal biology, and it is necessary to evaluate the efficiency, cost-effectiveness, and efficacy of different extraction methods to ensure successful amplification of the ...
Dar Gulam Jeelani   +5 more
doaj   +2 more sources

Global Spore Sampling Project: A global, standardized dataset of airborne fungal DNA. [PDF]

open access: goldSci Data
Ovaskainen O   +111 more
europepmc   +3 more sources

Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding? [PDF]

open access: goldIMA Fungus, 2020
Lücking R   +26 more
europepmc   +3 more sources

Molecular diagnostics in cancer patients with suspected respiratory mold infections [PDF]

open access: yesJournal of Clinical Microbiology
Invasive fungal infections (IFIs), including aspergillosis and mucormycosis, are serious complications in cancer patients. Reliable detection of etiologic agents is challenging but important for optimal patient management.
V. Rickerts   +23 more
doaj   +2 more sources

Optimized protocol for culturing and extracting DNA from fungal isolates associated with brown spot needle blight in pine trees. [PDF]

open access: yesPLoS ONE
Effective culturing and DNA extraction protocols are essential for advancing research on fungal pathogens of brown spot needle blight (BSNB) that infect loblolly pine (Pinus taeda) and other Pinus species. We evaluated the performance of four widely used
Temitope Ruth Folorunso   +6 more
doaj   +2 more sources

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