Results 41 to 50 of about 32,754 (167)

Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion.

PLoS Pathogens, 2021
Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) infects cells by binding to the host cell receptor ACE2 and undergoing virus-host membrane fusion.
Guido Papa, Donna L Mallery, Anna Albecka, Lawrence G Welch, Jérôme Cattin-Ortolá, Jakub Luptak, David Paul, Harvey T McMahon, Ian G Goodfellow, Andrew Carter, Sean Munro, Leo C James   +11 more
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Furin Egress from the TGN is Regulated by Membrane‐Associated RING‐CH Finger (MARCHF) Proteins and Ubiquitin‐Specific Protease 32 (USP32) via Nondegradable K33‐Polyubiquitination

Advanced Science
Furin primarily localizes to the trans‐Golgi network (TGN), where it cleaves and activates a broad range of immature proproteins that play critical roles in cellular homeostasis, disease progression, and infection.
Wenqiang Su, Iqbal Ahmad, You Wu, Lijie Tang, Ilyas Khan, Bowei Ye, Jie Liang, Sunan Li, Yong‐Hui Zheng   +8 more
doaj   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Lysyl oxidase (LOX)‐mediated remodeling of the extracellular matrix

FEBS Letters, EarlyView.
The lysyl oxidase (LOX) family of enzymes drives extracellular matrix remodeling by oxidizing collagen, elastin, and fibronectin, promoting covalent cross‐link formation. This activity regulates tissue architecture and mechanical properties, while its dysregulation contributes to fibrosis, cardiovascular disease, glaucoma, connective tissue disorders ...
Fernando Rodríguez‐Pascual, Marta Navarro‐Gutiérrez, Verónica Romero‐Albillo, Raquel Jiménez‐Sánchez   +3 more
wiley   +1 more source

Furin inhibition by furin-Eda peptide.

, 2013
Figure 5A. Progress curves showing inhibition of furin activity by furin-Eda-peptide (II). The assay was conducted using Boc-RVRR-MCA (20 µM) as fluorogenic substrate. Figure 5B. Dixon plots showing inhibition of furin activity by furin-Eda-peptide (II).
Abdel-Majid Khatib (209624), Subhendu Sekhar Bag (369012), Amit Basak (369013), Sarmistha Basak (369010), Maria Kolajova (369011), Ajoy Basak (369007), Dayani Mohottalage (369008)   +6 more
core   +1 more source

Canonical Antibodies Adopt Distinct Binding Modes to Recognize Viral Glycan Shields

Advanced Science, EarlyView.
Canonical Y‐shaped antibodies recognize viral glycan shields through adaptive Fab assembly states shaped by glycan organization and somatic hypermutation. Structural analyses ofbroadly neutralizing antibodies VRC35 and VRC36 across glycoproteins of HIV‐1, influenza, SARS‐CoV‐2, and Lassa viruses reveal distinct Fab assembly states, spanning monovalent,
Jiaxuan Cheng, Evan M. Cale, Amirabbas Maghsoudi, Matthew S. Sutton, Nancy S. Longo, Rebecca A. Gillespie, Lingshu Wang, Ivan Kosik, Sue Chong, Atsuhiro Yasuhara, Tatsiana Bylund, Arne Schön, Prabhanshu Tripathi, Yaroslav Tsybovsky, Haotian Lei, Nicholas C. Morano, Allison B. Lupatkin, Abraham J. Morton, Zabrina C. Lang, Jordan E. Becker, Isabella R. Frascilla, Myungjin Lee, Ning Li, Cuiping Liu, Ryan S. Roark, Chen‐Hsiang Shen, I‐Ting Teng, David J. Van Wazer, Danyi Wang, Lingyuan Wu, Goran Ahlsen, Mike Castro, Haijuan Du, Michael J. Ernandes, Tiansheng Li, Bob C. Lin, Mark K. Louder, Krisha McKee, Jonah S. Merriam, Sijy O'Dell, Li Ou, Sergei Pletnev, David Prikryl, Qi Qiu, Sarah Rubin, Mallika Sastry, Stephen D. Schmidt, Asif Shajahan, Andrea R. Shiakolas, Sanjay Srivatsan, Baoshan Zhang, Qiong Zhou, Mark Connors, Jason G. Gall, Yicheng Guo, Rick K. Huang, Yaoxing Huang, Richard A. Koup, Q. Paula Lei, John R. Mascola, Reda Rawi, Leonid Serebryannyy, Lawrence Shapiro, Zizhang Sheng, David D. Ho, Patrick C. Wilson, Jonathan W. Yewdell, Theodore C. Pierson, Masaru Kanekiyo, Nicole A. Doria‐Rose, Peter D. Kwong, Tongqing Zhou   +71 more
wiley   +1 more source

Interaction of furin-Eda peptide (II/IIa) with furin enzyme.

, 2013
Figure 10A. Docking of cyclized furin-Eda peptide (IIa) with furin P-domain (residue 487–560). Cyclized furin-Eda-peptide (IIa) is shown in space filled structure in red while the furin P-domain (residue 487Ala-Gln-Ala-Arg---------Ala-Asn-Asn-Tyr560) is ...
Abdel-Majid Khatib (209624), Subhendu Sekhar Bag (369012), Amit Basak (369013), Sarmistha Basak (369010), Maria Kolajova (369011), Ajoy Basak (369007), Dayani Mohottalage (369008)   +6 more
core   +1 more source

Switching Spike Plasticity Shapes ACE2 Engagement Across SARS‐CoV‐2 Variants

Advanced Science, EarlyView.
Conformational plasticity governs SARS‐CoV‐2 spike function and ACE2 recognition. Using high‐speed AFM and single‐molecule force spectroscopy, we analyzed the ancestral spike and nine variants, revealing progressive rigidification from Delta, variable plasticity in Omicron sublineages, and JN.1 compaction.
Sarah Stainer, Rong Zhu, Manuel Reithofer, Gerhard Stadlmayr, Katharina Stadlbauer, Panchika Prangkio, Christoph Rippel, Markus Hahn, Aniko Eva Kasztner, Stefanie Stohl, Petr Bednar, Daniel Ruzek, David Glück, Robert Tampé, Gordana Wozniak‐Knopp, Yoo Jin Oh, Miriam Klausberger, Peter Hinterdorfer   +17 more
wiley   +1 more source

Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein [PDF]

, 2010
Background: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes.
Wels, Winfried, Pranav Oberoi, Jabulowsky, Robert Alexander, Benhar, Itai, Benjamin Dälken, Oberoi, Pranav, Robert A Jabulowsky, Itai Benhar, Winfried S Wels, Dälken, Benjamin   +9 more
core   +1 more source