Results 161 to 170 of about 675,878 (209)
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Construction of Immunoglobulin Fusion Proteins
Current Protocols in Immunology, 2002AbstractRecombinant DNA technology has allowed the preparation of chimeric genes encoding proteins with novel properties. This unit describes the construction and subsequent testing of genes encoding immunoglobulin chimeras. The first protocol details fusion of a protein (or protein fragment) of interest onto an immunoglobulin constant region using a ...
Diane, Hollenbaugh, Alejandro, Aruffo
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Protein-Mediated Membrane Fusion
Annual Review of Biophysics and Biophysical Chemistry, 1989PERSPECTIVES AND OVERVIEW 187 GEN ERAL CON CEPTS 188 Fusion Versus Fission 188 Endoplasmic and Exoplasmic Fusion Events 189 Tightness of Fusion 190 PHYSICAL FORCES BETWEEN MEMBRAN ES ........ 190 FUSION ASSAyS 191 LlPOSOMES AS TARGET MEMBRANES 192 EXOPLASMIC FUSION 193 Influenza Virus Fusion 193 Acid-Induced Conformational Change in ...
T, Stegmann, R W, Doms, A, Helenius
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Bone Morphogenetic Protein and Fusion
Journal of Neurosurgery: Spine, 2007In their article (Villavicencio AT, Burneikiene S, Nelson EL, et al: Safety of transforaminal lumbar interbody fusion and intervertebral recombinant human bone morphogenetic protein– 2. J Neurosurg Spine 3: 436– 443, December, 2005), Villavicencio and colleagues referred to the work by Haid et al., claiming that the fusion rates during ...
Pećina, Marko +2 more
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Diphtheria Toxin Fusion Proteins
1998Two different approaches have been undertaken to develop targeted biomolecules for therapeutics. The first was the construction of immunotoxins consisting of monoclonal antibodies chemically linked through a disulfide bond to a plant or bacterial toxin or radionuclide.
F M, Foss +4 more
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Antibody–cytokine fusion proteins
Archives of Biochemistry and Biophysics, 2012Cytokines are key players in stimulating and regulating immune responses in physiological and pathophysiological processes. Various cytokines have been approved for therapy of cancer and other diseases and many more are under development. However, therapeutic efficacy is often hampered by severe side effects and poor pharmacokinetic properties.
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Preparation of Insoluble GST Fusion Proteins
Cold Spring Harbor Protocols, 2009INTRODUCTIONA wide variety of plasmid vectors are commercially available for the production of fusion proteins in bacterial cells. Most are also designed to incorporate a tag that allows affinity purification of the expressed fusion protein from bacterial cell extracts.
Ilaria, Rebay, Richard G, Fehon
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Functional Classification of Fusion Proteins in Sarcoma [PDF]
CancersMarco Wachtel +2 more
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Quantitative lmmunoblotting with Fusion Proteins
2003The development of vaccines against HIV-1 is currently hindered by incomplete understanding of correlates of protective immunity (1-3). Experiments are necessary to measure immune responses in sufficiently fine detail that specific protective responses can be discerned from those that are irrelevant or harmful.
M M, Mitchell, L D, Loomis-Price
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Cytokine Fusion Protein Treatment
2002Mice suffering from melanoma can be cured by treatment with a recombinant antibody-lymphotoxin-alpha fusion protein. In order to characterize the involvement of T cells in this syngeneic tumor model, the T cell receptor repertoire was analyzed both quantitatively and qualitatively.
David, Schrama +4 more
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Design of fusion proteins for biocatalysis
The use of enzymes to convert substrates into valuable products has been an integral part of biocatalysis. However, some reactions are energy-demanding that requires the use of NAD(P)H to proceed. This NAD(P)H can be costly impeding the progress of enzyme usage at a bigger scale.Beyzanur, Celebi +3 more
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