Results 41 to 50 of about 16,922,871 (257)

Targeting G protein-coupled receptor for pain management

Brain Circulation, 2017
Pain is an unpleasant sensory and emotional experience associated with actual or potential tissue damage or described in terms of such damage. Great progress has been made in understanding the important roles of various G protein-coupled receptors in the
Hongyan Li, Rong Wang, Yinying Lu, Xuehua Xu, Jiaxiang Ni   +4 more
doaj   +1 more source

A Robust and Rapid Method of Producing Soluble, Stable, and Functional G-Protein Coupled Receptors [PDF]

, 2011
Membrane proteins, particularly G-protein coupled receptors (GPCRs), are notoriously difficult to express. Using commercial E.coli cell-free systems with the detergent Brij-35, we could rapidly produce milligram quantities of 13 unique GPCRs ...
Braun, Dieter, Shuguang Zhang, Baaske Philipp, Moran Jerabek-Willemsen, Geissler, Sandra, Duhr, Stefan, Duhr Stefan, Corin, Karolina A., Sandra Geissler, Wienken Christoph J., Christoph J. Wienken, Geissler Sandra, Jerabek-Willemsen, Moran, Christoph J Wienken, Zhang Shuguang, Stefan Duhr, Emily Brown, Wang Xiaoqiang, Philipp Baaske, Deepali B. Ravel, Brown, Emily, Song Junyao, Xiaoqiang Wang, Braun Dieter, Karolina Corin, Junyao Song, Wang, Xiaoqiang, Dieter Braun, Baaske, Philipp, Wienken, Christoph J., Ravel Deepali B., Corin, Karolina, Ravel, Deepali B., Brown Emily, Song, Junyao, Jerabek-Willemsen Moran, Corin Karolina, Zhang, Shuguang, Deepali B Ravel, Brown, Emily E., Ra Geissler   +40 more
core   +1 more source

Heterodimerization of apelin receptor and neurotensin receptor 1 induces phosphorylation of ERK1/2and cell proliferationviaGαq-mediated mechanism [PDF]

, 2014
Dimerization of G protein-coupled receptors (GPCRs) is crucial for receptor function including agonist affinity, efficacy, trafficking and specificity of signal transduction, including G protein coupling.
Cai, X, Jiang, Y, Karteris, E, Bai, B, Chen, J, Karteris, Emmanouil, Bai, Bo, Jiang, Yunlu, Cai, Xin, Chen, Jing   +9 more
core   +1 more source

High capacity in G protein-coupled receptor signaling

Nature Communications, 2018
G protein-coupled receptors (GPCRs) activate intracellular signalling pathways upon extracellular stimulation. Here authors record single cell responses of GPCR signalling which allows the direct estimation of its channel capacity for each cell along ...
Amiran Keshelava, Gonzalo P. Solis, Micha Hersch, Alexey Koval, Mikhail Kryuchkov, Sven Bergmann, Vladimir L. Katanaev   +6 more
doaj   +1 more source

Serum Myonectin Levels Are Positively Associated With Physical Function and Lower Frailty‐Related Limitation in Maintenance Hemodialysis Patients: A Cross‐Sectional Study

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Background Maintenance hemodialysis (MHD) patients frequently suffer from frailty, characterized by reduced physical function and poor prognosis. Myokines, such as myonectin, secreted by muscle, are emerging regulators of systemic health. This study investigated the relationship between serum myonectin, adipokines (adiponectin, omentin), and ...
Kenichi Kono, Yoshifumi Moriyama, Koji Ohashi, Naoya Otaka, Minako Tatsumi, Toyoaki Murohara, Noriyuki Ouchi, Satoru Oshima   +7 more
wiley   +1 more source

G protein-coupled receptor kinase 2-mediated phosphorylation of ezrin is required for G protein-coupled receptor-dependent reorganization of the actin cytoskeleton [PDF]

, 2005
G protein-coupled receptor kinase 2 (GRK2) phosphorylates and desensitizes activated G protein-coupled receptors (GPCRs). Here, we identify ezrin as a novel non-GPCR substrate of GRK2. GRK2 phosphorylates glutathione S-transferase (GST)-ezrin, but not an
Pitcher, JA, Cant, SH
core  

Fluorescent leukotriene B4: potential applications

Journal of Lipid Research, 2005
Leukotriene B4 (LTB4) is a potent lipid mediator of inflammation that acts primarily via a seven-transmembrane-spanning, G-protein-coupled receptor denoted BLT1. Here, we describe the synthesis and characterization of fluorescent analogs of LTB4 that are
Alan Sabirsh, Anders Wetterholm, Jesper Bristulf, Hakon Leffler, Jesper Z. Haeggström, Christer Owman   +5 more
doaj   +1 more source

Comparative Evaluation of Hemodiafiltration, Hemoperfusion, and Standard Hemodialysis on Efficacy, Inflammatory Control, Dialysis Adequacy, and Safety in End‐Stage Renal Disease: A Prospective Observational Study

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Background Chronic micro‐inflammation in patients with end‐stage renal disease (ESRD) is a significant driver of cardiovascular complications and diminished quality of life. While standard hemodialysis (SHD) effectively manages small‐molecule clearance, its ability to remove medium‐to‐large uremic toxins—the primary catalysts of systemic ...
Hongwei Zuo, Weisi Dai, Jie Mao, Mei Ye, Xingshan Lu, Xiaocui Zhang   +5 more
wiley   +1 more source

Chemically engineering ligand selectivity at the free fatty acid receptor 2 based on pharmacological variation between species orthologs [PDF]

, 2012
When it is difficult to develop selective ligands within a family of related G-protein-coupled receptors (GPCRs), chemically engineered receptors activated solely by synthetic ligands (RASSLs) are useful alternatives for probing receptor function. In the
Christiansen, Elisabeth, Tikhonova, I.G., Ulven, T., Hudson, B., Tikhonova, Irina G, Adams, D.R., Grundmann, Manuel, Milligan, Graeme, Hudson, Brian D, Christiansen, E., Kostenis, Evi, Kostenis, E., Adams, David R, Milligan, G., Tikhonova, Irina G; id_orcid, Ulven, Trond, Grundmann, M.   +16 more
core   +1 more source

A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties

, 2022
S.57-77Fluorescent labeling of de novo synthesized proteins is in particular a valuable tool for functional and structural studies of membrane proteins.
Thoring, L., Richter, T., Kubick, S., Zemella, A.   +3 more
core   +1 more source