Results 61 to 70 of about 16,618,960 (281)
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Interaction of alpha2-Adrenergic Receptors With G-Proteins [PDF]
, 1991 A wide variety of hormones and neurotransmitters have been shown to produce intracellular signals mediated by guanine-nucleotide binding proteins (G-proteins).
McClue, Steven J
core +5 more sources
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
, 2012 The classical view of heterotrimeric G protein signaling places G -proteins at the cytoplasmic surface of the cell's plasma membrane where they are activated by an appropriate G protein-coupled receptor. Once activated, the GTP-bound Gα and the free Gβγ are able to regulate plasma membrane-localized effectors, such as adenylyl cyclase, phospholipase C ...
openaire +2 more sources
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
Frizzled Proteins are bona fide G Protein-Coupled Receptors [PDF]
, 2009 Receptors of the Frizzled family initiate Wnt ligand-dependent signaling controlling multiple steps in organism development and highly conserved in evolution. Misactivation of the Wnt/Frizzled signaling is cancerogenic.
Silke Buestorf, Vladimir L. Katanaev
core
APPLICATION OF IMMUNOGLOBULIN-BINDING PROTEINS A, G, L IN THE AFFINITY CHROMATOGRAPHY [PDF]
Biotechnologia Acta, 2014 Proteins A, G and L are native or recombinant proteins of microbial origin that bind to mammalian immunoglobulins. Preferably recombinant variants of proteins A, G, L are used in biotechnology for affinity sorbents production. Сomparative characteristics
О. V. Sviatenko, O. B. Gorbatiuk
doaj +1 more source
STRUCTURE AND FUNCTION OF G PROTEINS
Pharmacology & Therapeutics, 1991 G proteins are heterotrimeric proteins involved in the transduction of a variety of external signals in all eukaryotic organisms. This review analyzes the molecular aspects of G protein structure and function. The cloning of cDNAs coding for a great variety of G protein subunits has allowed us to deduce the primary and secondary structure of the ...
Olate, Juan, Allende, Jorge E.
openaire +3 more sources
Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential
FEBS Letters, EarlyView.Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta +3 more
wiley +1 more source
Nuclear and cytoskeletal translocation and localization of heterotrimeric G-proteins
, 2015 Heterotrimeric GTP-binding proteins (G-proteins) are involved in a diverse array of signalling path-ways. They are generally thought to be membrane-bound proteins, which disassociate on receptor activation and binding of GTP.
Crouch, Michael F
core +1 more source