Results 71 to 80 of about 10,328 (211)

Highlighting the hidden: monitoring the avidity-driven association of a fluorescent GABARAP tandem with microtubules in living cells

Autophagy Reports
GABARAP, like other ATG8 proteins, is a ubiquitin-like modifier and its C-terminal lipid conjugation enables association with cellular membranes. To prevent interference with the lipidation process, N-terminal fluorescent protein (FP) tagging strategies ...
Alina Üffing, Lisa Gold, Thomas Gensch, Oliver H. Weiergräber, Silke Hoffmann, Dieter Willbold   +5 more
doaj   +1 more source

Ciliary Type III Adenylyl Cyclase in the VMH Is Crucial for High‐Fat Diet‐Induced Obesity Mediated by Autophagy

Advanced Science, 2022
Neuronal primary cilia are crucial for body weight maintenance. Type III adenylyl cyclase (AC3) is abundantly enriched in neuronal cilia, and mice with global AC3 ablation are obese.
Dong Yang, Xiangbo Wu, Weina Wang, Yanfen Zhou, Zhenshan Wang   +4 more
doaj   +1 more source

AlphaFold2‐Guided Cyclic Peptide Stabilizer Design to Target Protein–Protein Interactions

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT The control and modulation of protein–protein interactions (PPIs) is of central importance for the majority of biological processes and most biomedical applications. Stabilization of PPIs, besides inhibition, is of growing pharmaceutical interest.
Niklas Halbwedl, Martin Zacharias
wiley   +1 more source

PACS2 Alleviates Sepsis‐Induced Myopathy by Activating ERK–MAPK Signalling Pathway to Suppress ER‐Phagy

Journal of Cachexia, Sarcopenia and Muscle, Volume 17, Issue 3, June 2026.
ABSTRACT Background Sepsis‐induced myopathy (SIM) is a common and life‐threatening complication, but its underlying mechanisms remain poorly understood. PACS2, a key resident protein at mitochondria‐associated endoplasmic reticulum membranes (MAMs), regulates ER homeostasis under various pathological conditions.
Xuexin Li, Zu‐an Shi, Fei He, Guo Mu, Feixiang Wang, Bowen Sun, Xiaobin Wang, Li Liu   +7 more
wiley   +1 more source

Alternative splicing enriched cDNA libraries identify breast cancer-associated transcripts [PDF]

, 2010
Background: Alternative splicing (AS) is a central mechanism in the generation of genomic complexity and is a major contributor to transcriptome and proteome diversity.
Elisa N Ferreira, Maria CR Rangel, Pedro F Galante, Jorge E de Souza, Gustavo C Molina, Sandro J de Souza, Dirce M Carraro   +6 more
core   +2 more sources

Gene Deletion of Gabarap Enhances Nlrp3 Inflammasome-Dependent Inflammatory Responses [PDF]

The Journal of Immunology, 2013
Abstract The γ-aminobutyric acid A receptor–associated protein (Gabarap) functions in γ-aminobutyric acid A receptor trafficking and postsynaptic localization in neurons, but its physiological roles in other systems have not been studied. In this study, we report that Gabarap-deficient mice are more susceptible to mortality in two sepsis
Zhang, Zhirong, Xu, Xiaozheng, Ma, Jianhui, Wu, Jianfeng, Wang, Yanhai, Zhou, Rongbin, Han, Jiahuai   +6 more
openaire   +3 more sources

The Dual Role of Autophagy in Cancer: Mechanisms and Therapeutic Strategies

MedComm – Oncology, Volume 5, Issue 2, June 2026.
Autophagy is a conserved cellular process degrading dysfunctional organelles and protein aggregates to maintain cell homeostasis, and it exhibits context‐dependent duality in cancer. Autophagy functions as a critical tumor‐suppressive mechanism by preventing DNA damage and mutation during tumor initiation.
Xiang‐Zheng Gao, Yuheng Qin, Haoyu Wan, Guang Lu, Han‐Ming Shen   +4 more
wiley   +1 more source

ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1

Autophagy, 2023
TBK1 (TANK binding kinase 1) is an essential kinase of antiviral immunity, yet the regulatory mechanisms responsible for its stringent control via autophagy are not fully understood. Here, we identify the macroautophagy/autophagy-related cysteine protease ATG4B as a negative regulator of human antiviral immune responses by targeting TBK1 for autophagic
Weihong Xie, Chenqiu Zhang, Zheyu Wang, Hui Chen, Tonghui Gu, Tao Zhou, Yaoxing Wu, Fan Xia, Min Li, Jun Wang, Renjie Jiao, Jun Cui, Shouheng Jin   +12 more
openaire   +2 more sources

Molecular determinants regulating selective binding of autophagy adapters and receptors to ATG8 proteins

Nature Communications, 2019
Autophagy adaptors and receptors contain LC3-interacting region (LIR) motifs that bind selectively to the LIR docking site of GABARAP and other members of the ATG8 family. Here the authors show that in addition to the LIR motif also the region C-terminal
Martina Wirth, Wenxin Zhang, Minoo Razi, Lynet Nyoni, Dhira Joshi, Nicola O’Reilly, Terje Johansen, Sharon A. Tooze, Stéphane Mouilleron   +8 more
doaj   +1 more source

Insights on autophagosome–lysosome tethering from structural and biochemical characterization of human autophagy factor EPG5

Communications Biology, 2021
Nam and Cheung et al. describe the structural and biochemical characterization of human autophagy factor EPG5 that functions in autophagosome–lysosome tethering.
Sung-Eun Nam, Yiu Wing Sunny Cheung, Thanh Ngoc Nguyen, Michael Gong, Samuel Chan, Michael Lazarou, Calvin K. Yip   +6 more
doaj   +1 more source