Continuous Packed Bed Reactor with Immobilized β-Galactosidase for Production of Galactooligosaccharides (GOS) [PDF]
The β-galactosidase from Bacillus circulans was covalently attached to aldehyde-activated (glyoxal) agarose beads and assayed for the continuous production of galactooligosaccharides (GOS) in a packed-bed reactor (PBR).
Ballesteros Olmo, Antonio +4 more
core +1 more source
Biotechnology of Lactulose Production: Progress, Challenges, and Prospects
Lactulose is a prebiotic that has found a wide application in medicine and food industry. Commercial lactulose is usually synthesized by isomerization in alkaline media at high temperatures.
Svetlana A. Ryabtseva +6 more
doaj +1 more source
Biotechnological applications of functional metagenomics in the food and pharmaceutical industries [PDF]
peer-reviewedMicroorganisms are found throughout nature, thriving in a vast range of environmental conditions. The majority of them are unculturable or difficult to culture by traditional methods.
Alvarez-Ordonez, Avelino +3 more
core +1 more source
Lactulose Biosynthesis with Enzymes of Lactose-Fermenting Micromycetes and Bacteria
Lactulose is a well-studied prebiotic popular in medicine and the food industry. β-Galactosidases offer an alternative to traditional chemical methods of lactulose production. The article describes lactulose biosynthesis with crude enzyme preparations of
Svetlana A. Ryabtseva +6 more
doaj +1 more source
Present status on removal of raffinose family oligosaccharides - a Review
Raffinose family oligosaccharides (RFOs) are α-galactosyl derivatives of sucrose or glucose. They are found in a large variety of seeds from many different families such as beans, vegetables and whole grains.
Jian Zhang +5 more
doaj +1 more source
Expression and purification of β-galactosidases derived from Bifidobacterium provide a new resource for efficient lactose hydrolysis and lactose intolerance alleviation. Here, we cloned and expressed two β-galactosidases derived from Bifidobacterium. The
Mingzhu Du +9 more
doaj +1 more source
Overview of molecular signatures of senescence and associated resources: pros and cons
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas +6 more
wiley +1 more source
Senescence-associated beta-galactosidase is lysosomal beta-galactosidase
Replicative senescence limits the proliferation of somatic cells passaged in culture and may reflect cellular aging in vivo. The most widely used biomarker for senescent and aging cells is senescence-associated beta-galactosidase (SA-beta-gal), which is defined as beta-galactosidase activity detectable at pH 6.0 in senescent cells, but the origin of SA-
LEE BY +8 more
openaire +4 more sources
Biocatalytic approaches using lactulose: end product compared with substrate [PDF]
Lactulose is a lactose-based carbohydrate with well-known prebiotic effect and recognized medical applications. Currently, the commercially available lactulose is chemically synthesized. Nevertheless, the process leads to low yields and high levels of by-
Macedo, Eugénia A. +3 more
core +2 more sources
Construction, Isolation and Implications of Repressor‐galactosidase ·ß‐galactosidase Hybrid Molecules [PDF]
Escherichia coli heterogenotes, which produce hybrid molecules between the chimaeric protein repressor‐galactosidase and the enzyme β‐galactosidase, were constructed. Repressor‐galactosidase. in which fully active lac repressor is covalently linked to active β‐galactosidase, is an aggregate with a core structure of four β‐galactosidase parts and two ...
J, Kania, B, Müller-Hill
openaire +2 more sources

