Synthesis of prebiotic galactooligosaccharides from lactose using bifidobacterial β-galactosidase (BbgIV) immobilised on DEAE-Cellulose, Q-Sepharose and amino-ethyl agarose [PDF]
, 2013 The bifidobacterial β-galactosidase BbgIV was immobilised on DEAE-Cellulose and Q-Sepharose via ionic binding and on amino-ethyl- and glyoxal-agarose via covalent attachment, and was then used to catalyse the synthesis of galactooligosaccharides (GOS).
Albayrak +41 more
core +1 more source
Bioconversion of Beet Molasses to Alpha-Galactosidase and Ethanol
Frontiers in Microbiology, 2019 Molasses are sub-products of the sugar industry, rich in sucrose and containing other sugars like raffinose, glucose, and fructose. Alpha-galactosidases (EC.
María-Efigenia Álvarez-Cao +3 more
doaj +1 more source
Functional metagenomics reveals novel β-galactosidases not predictable from gene sequences. [PDF]
PLoS ONE, 2017 The techniques of metagenomics have allowed researchers to access the genomic potential of uncultivated microbes, but there remain significant barriers to determination of gene function based on DNA sequence alone.
Jiujun Cheng +6 more
doaj +1 more source
Towards broad spectrum activity-based glycosidase probes: synthesis and evaluation of deoxygenated cyclophellitol aziridines [PDF]
, 2017 Activity-based protein profiling has emerged as a powerful tool for visualizing glycosidases in complex biological samples. Several configurational cyclophellitol isomers have been shown to display high selectivity as probes for glycosidases processing ...
Adriaan J. Minnaard +39 more
core +3 more sources
Biotechnology of Lactulose Production: Progress, Challenges, and Prospects
Техника и технология пищевых производств, 2023 Lactulose is a prebiotic that has found a wide application in medicine and food industry. Commercial lactulose is usually synthesized by isomerization in alkaline media at high temperatures.
Svetlana A. Ryabtseva +6 more
doaj +1 more source
Discovery of novel enzymes with industrial potential from a cold and alkaline environment by a combination of functional metagenomics and culturing [PDF]
, 2014 BACKGROUND: The use of cold-active enzymes has many advantages, including reduced energy consumption and easy inactivation. The ikaite columns of SW Greenland are permanently cold (4-6°C) and alkaline (above pH 10), and the microorganisms living there ...
Jan Vester +2 more
core +1 more source
Present status on removal of raffinose family oligosaccharides - a Review
Czech Journal of Food Sciences, 2019 Raffinose family oligosaccharides (RFOs) are α-galactosyl derivatives of sucrose or glucose. They are found in a large variety of seeds from many different families such as beans, vegetables and whole grains.
Jian Zhang +5 more
doaj +1 more source
Molecules, 2022 Expression and purification of β-galactosidases derived from Bifidobacterium provide a new resource for efficient lactose hydrolysis and lactose intolerance alleviation. Here, we cloned and expressed two β-galactosidases derived from Bifidobacterium. The
Mingzhu Du +9 more
doaj +1 more source
Deep UV plasmonic enhancement of single protein autofluorescence in zero-mode waveguides [PDF]
, 2019 Single molecule detection provides detailed information about molecular structures and functions, but it generally requires the presence of a fluorescent marker which can interfere with the activity of the target molecule or complicate the sample production.
arxiv +1 more source
Biologia Plantarum, 2018 This work studied the six β-galactosidases (BGALs) of the subfamily a1 of Arabidopsis, that have been proposed to play important roles in the cell wall remodelling during plant development, although their precise functions are still unknown.
M. Moneo-Sánchez +6 more
doaj +1 more source