Improving the Secretory Expression of an -Galactosidase from Aspergillus niger in Pichia pastoris. [PDF]
α-Galactosidases are broadly used in feed, food, chemical, pulp, and pharmaceutical industries. However, there lacks a satisfactory microbial cell factory that is able to produce α-galactosidases efficiently and cost-effectively to date, which prevents ...
Xianliang Zheng +6 more
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Senescence‐associated β‐galactosidase is lysosomal β‐galactosidase [PDF]
SummaryReplicative senescence limits the proliferation of somatic cells passaged in culture and may reflect cellular aging in vivo. The most widely used biomarker for senescent and aging cells is senescence‐associated β‐galactosidase (SA‐β‐gal), which is defined as β‐galactosidase activity detectable at pH 6.0 in senescent cells, but the origin of SA‐β‐
Bo Yun Lee +8 more
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Characterization of GH2 and GH42 β-galactosidases derived from bifidobacterial infant isolates
Bifidobacteria are among the first and most abundant bacterial colonizers of the gastrointestinal tract of (breast-fed) healthy infants. Their success of colonising the infant gut is believed to be, at least partly, due to their ability to metabolize ...
Valentina Ambrogi +7 more
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Cerebroside galactosidase of brain
The galactoside bond in cerebroside was found to be cleaved by an enzyme in rat and pig brain. Emulsified stearoyl-(14)C psychosine was used as the substrate and the extent of cleavage was studied by isolating and counting the stearoyl sphingosine (ceramide) formed.
Amiya K. Hajra +3 more
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Functional metagenomics reveals novel β-galactosidases not predictable from gene sequences. [PDF]
The techniques of metagenomics have allowed researchers to access the genomic potential of uncultivated microbes, but there remain significant barriers to determination of gene function based on DNA sequence alone.
Jiujun Cheng +6 more
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Bioconversion of Beet Molasses to Alpha-Galactosidase and Ethanol
Molasses are sub-products of the sugar industry, rich in sucrose and containing other sugars like raffinose, glucose, and fructose. Alpha-galactosidases (EC.
María-Efigenia Álvarez-Cao +3 more
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Lactulose Biosynthesis with Enzymes of Lactose-Fermenting Micromycetes and Bacteria
Lactulose is a well-studied prebiotic popular in medicine and the food industry. β-Galactosidases offer an alternative to traditional chemical methods of lactulose production. The article describes lactulose biosynthesis with crude enzyme preparations of
Svetlana A. Ryabtseva +6 more
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Biochemical characterization of digestive carbohydrases in Moroccan locust, Dociostaurus maroccanus Thunberg (Orthoptera: Acrididae) [PDF]
The Moroccan locust, Dociostaurus maroccanus (Thunberg), is an agricultural pest which has caused considerable agricultural damage and economic importance.
Bahareh Rafiei +2 more
doaj
A novel Arthrobacter beta-galactosidase with homology to eucaryotic beta-galactosidases
An Arthrobacter beta-galactosidase has homology with the lysosomal acid beta-galactosidases from humans and mice and with a Xanthomonas manihotis enzyme. Phylogenetic analysis of the deduced amino acid sequence showed an unusual pattern, with this procaryotic enzyme clustering within the animal clade.
K, Gutshall, K, Wang, J E, Brenchley
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Present status on removal of raffinose family oligosaccharides - a Review
Raffinose family oligosaccharides (RFOs) are α-galactosyl derivatives of sucrose or glucose. They are found in a large variety of seeds from many different families such as beans, vegetables and whole grains.
Jian Zhang +5 more
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