Results 151 to 160 of about 9,031 (203)
Inhibition of gamma-glutamyl hydrolases in human cells by 2-mercaptomethylglutaric acid
Biochemical and Biophysical Research Communications, 1987 Cultured human lymphocytes and fibroblasts accumulate methotrexate during 24 hours and synthesize methotrexate polyglutamates up to the hexaglutamate, with the triglutamate predominating. In the interval after incubation with methotrexate, drug is lost, metabolites are converted to longer chain-lengths, and methotrexate pentaglutamate predominates.
V. Michael Whitehead +2 more
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The measurement of gamma-glutamyl hydrolase (conjugase) activity in rat brain
Neurochemical Research, 1988 An assay using the artificial substrate, 2,4-diamino-10-methyl-pteroylglutamyl-gamma-glutamate (MTX-G1), was developed to measure gamma-glutamyl hydrolase (conjugase), which hydrolyzes folylpolyglutamates. This assay allows us to: 1) measure conjugase for the first time in rat brain and 2) measure conjugase in a reliable, sensitive and inexpensive ...
Dean M. Hartley +2 more
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Molecular Pharmacology, 1995 A subline of H35 hepatoma cells (H35D cells) that have been made resistant to 5,10-dideazatetrahydrofolate exhibits an increase in gamma-glutamyl hydrolase (GH) activity. GH is a lysosomal enzyme in H35 and H35D cells on the basis of comparison of the distribution of enzyme activity with other known lysosomal enzymes.
Rong Yao, Myung S. Rhee, John Galivan
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The properties and function of gamma-glutamyl hydrolase and poly-gamma-glutamate.
Advances in enzyme regulation, 1993gamma-Glutamyl hydrolase is a ubiquitous enzyme that has the capacity to cleave gamma-glutamyl bonds of cellular folyl- and antifolylpoly-gamma-glutamates. This study has revealed that the enzyme is secreted by primary cultures of rat hepatocytes and by H35 hepatoma cells.
Y, Wang +6 more
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A rapid colorimetric assay for γ-glutamyl hydrolase (conjugase)
Analytical Biochemistry, 1992A simple procedure for the measurement of gamma-glutamyl hydrolase (conjugase) activity is described. Glutamic acid released from pteroylpenta-gamma-glutamate by hog kidney and chicken pancreas conjugases was quantitated using the dye 4,4'-bis(dimethylamino)benzophenone hydrazone.
P V, Wagh, T I, Kalman
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Structural organization of the human γ-glutamyl hydrolase gene
Gene, 1999Gamma-glutamyl hydrolase (GH) plays an important role in the metabolism of folic acid and the pharmacology of antifolates such as methotrexate. We have previously cloned and characterized the human GH cDNA. In this report, the complete organization and structure of the human GH gene was determined.
D, Yin +4 more
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Evaluation of phosphorus-containing inhibitors of γ-glutamyl hydrolase
Bioorganic & Medicinal Chemistry Letters, 1998Several putative, phosphorus-containing inhibitors of gamma-glutamyl hydrolase were synthesized and evaluated for inhibitory activity. The phosphonamidoic acids were shown to be weak competitive inhibitors while both a phosphoramidate diester and a phosphonamidate ester were shown to be potent time-dependent inactivators, presumably through ...
C E, Rodriguez +4 more
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Current Drug Metabolism, 2023
Background: Methotrexate (MTX) is a common folic acid antagonist in clinical medicine, easily inducing a common adverse side effect of liver and kidney injury. It has been found that the expression of Folylpolyglutamate Synthetase (FPGS) and gamma-Glutamyl Hydrolase (GGH) may be closely related to that of related proteins to affect the intracellular ...
Peng Yu +10 more
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Background: Methotrexate (MTX) is a common folic acid antagonist in clinical medicine, easily inducing a common adverse side effect of liver and kidney injury. It has been found that the expression of Folylpolyglutamate Synthetase (FPGS) and gamma-Glutamyl Hydrolase (GGH) may be closely related to that of related proteins to affect the intracellular ...
Peng Yu +10 more
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Die Pharmazie, 2019 Gamma-Glutamyl hydrolase (GGH) plays an important role in the disposition of anti-folate analogs. Several studies noted the pharmacological relevance of rs3758149 C/T polymorphism located in the human GGH promoter. The present study aimed to investigate the role of rs3758149 C/T polymorphism and transcription factors in the regulation of GGH expression
Shu‐Mei Wang +4 more
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