Results 161 to 170 of about 9,031 (203)

Acquisition of resistance to antifolates caused by enhanced gamma-glutamyl hydrolase activity.

Cancer research, 1993
A subline of H35 hepatoma cells has been developed which exhibits 80-fold resistance to 5,10-dideazatetrahydrofolate, an antifolate which inhibits glycinamideribonucleotide transformylase. The cells are cross-resistant to methotrexate, an inhibitor of dihydrofolate reductase and 10-propargyl-5,8-dideazafolate and its 2-desamino-2-methyl derivative ...
Myung S. Rhee, Ying Wang, Muraleedharan G. Nair, John Galivan   +3 more
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Characterization of human cellular gamma-glutamyl hydrolase.

Molecular pharmacology, 1998
A previously identified cDNA encoding a human gamma-glutamyl hydrolase was expressed in a baculovirus system. The expressed protein had molecular mass of 37 kDa. Treatment of the protein with PNGase F produced a protein of molecular mass of 30 kDa, indicating that the protein contained asparagine-linked glycosylation. Sequence analysis of the expressed
Myung S. Rhee, Barbara Lindau‐Shepard, Karen J. Chave, John Galivan, Thomas J. Ryan   +4 more
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Folylpolyglutamate synthase and gamma-glutamyl hydrolase regulate leucovorin-enhanced 5-fluorouracil anticancer activity.

Biochemical and Biophysical Research Communications, 2008
Although 5-fluorouracil (5-FU) plus leucovorin (LV) is a standard chemotherapy regimen for colorectal cancer, the factors that determine the LV-mediated enhancement of the antitumor activity of 5-FU have remained unknown. We investigated the roles of folylpolyglutamate synthase (FPGS) and gamma-glutamyl hydrolase (GGH), which are the main enzymes ...
Etsuko Sakamoto, Sayaka Tsukioka, Shinji Oie, Takashi Kobunai, Hiroaki Tsujimoto, Kazuki Sakamoto, Yoshihiro Okayama, Yoshikazu Sugimoto, Toshinori Oka, Masakazu Fukushima, Tatsuzo Oka   +10 more
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Secretion of gamma-glutamyl hydrolase in vitro.

Cancer research, 1991
gamma-Glutamyl hydrolase (also known as conjugase) is a ubiquitous enzyme that has the capacity to cleave folyl- and antifolylpolyglutamates. This study has revealed that the enzyme is secreted by primary cultures of rat hepatocytes and by H35 hepatoma cells.
B M, O'Connor, R F, Rotundo, Z, Nimec, J J, McGuire, J, Galivan   +4 more
openaire   +1 more source

In situ determination of the molecular weight of hepatic γ-glutamyl transferase and γ-glutamyl hydrolase activities

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1982
The molecular weight of gamma-glutamyl transferase from normal rat liver and hepatoma tissue was determined by radiation-inactivation and found to be approx 100000 in each case. The molecular weight previously reported for the subunit containing the gamma-glutamyl binding site (22000) is considerably less than that of the holoenzyme, suggesting that in
J L, Ding, G D, Smith, A, Searle, T J, Peters   +3 more
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Effects of overexpression of gamma-Glutamyl hydrolase on methotrexate metabolism and resistance.

Cancer research, 2001
Intracellular metabolism of methotrexate (MTX) to MTX-polyglutamates (MTXPG) is one determinant of cytotoxicity. Steady-state accumulation of MTXPG seems to depend on the activity of two enzymes: folylpolyglutamate synthetase (FPGS), which adds glutamate residues, and gamma-glutamyl hydrolase (GGH), which removes them.
Peter D. Cole, Barton A. Kamen, Richard Görlick, Debarbrata Banerjee, Angela K. Smith, Elizabeth Magill, Joseph R. Bertino   +6 more
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Proliferation-dependency of γ-glutamyl hydrolase activity in various mouse cells

Cell Biology International Reports, 1992
The activity of gamma-glutamyl hydrolase, the enzyme which deglutamylates folyl and antifolyl polyglutamates, changed significantly in mouse cells during different phases of growth, being about two times lower in actively proliferating mice splenocytes and fibroblasts than in nondividing cells.
E, Sikora, B, Kaminska, B, Grzelakowska-Sztabert   +2 more
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A central role for gamma-glutamyl hydrolases in plant folate homeostasis.

The Plant journal : for cell and molecular biology, 2011
Most cellular folates carry a short poly-γ-glutamate tail, and this tail is believed to affect their efficacy and stability. The tail can be removed by γ-glutamyl hydrolase (GGH; EC 3.4.19.9), a vacuolar enzyme whose role in folate homeostasis remains unclear.
Tariq A, Akhtar, Giuseppe, Orsomando, Payam, Mehrshahi, Aurora, Lara-Núñez, Malcolm J, Bennett, Jesse F, Gregory, Andrew D, Hanson   +6 more
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The properties of the secreted γ-glutamyl hydrolases from H35 hepatoma cells

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1993
gamma-Glutamyl hydrolase has been partially purified and characterized from conditioned culture medium of H35 hepatoma cells. Evidence for heterogeneity of the enzyme is derived from its elution as three distinct peaks of enzymatic activity when the enzyme is purified by TSK-butyl-Sepharose column chromatography.
Y, Wang, Z, Nimec, T J, Ryan, J A, Dias, J, Galivan   +4 more
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Properties of Folic Acid γ-Glutamyl Hydrolase (Conjugase) in Rat Bile and Plasma

The Journal of Nutrition, 1981
The properties of folic acid gamma-glutamyl hydrolase (conjugase) [EC 3.4.12.10] in rat bile and plasma were investigated. Conjugase activity in bile showed two pH optima; one at pH 4.5-5.0 and one at pH 6.7-7.5. The enzyme activity in plasma had a broad pH--profile with an optimum at pH 6.2-7.5.
D W, Horne, C L, Krumdieck, C, Wagner
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