Results 51 to 60 of about 741,703 (306)

Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity

Molecular Oncology, EarlyView.
Peroxidasin (PXDN) is secreted by melanoma cells and binds the NK cell receptor NKG2D, thereby suppressing NK cell activation and cytotoxicity. PXDN depletion restores NKG2D signaling and enables effective NK cell–mediated melanoma killing. These findings identify PXDN as a previously unrecognized immune evasion factor and a potential target to improve
Hsu‐Min Sung, David Bickel, Lena C. M. Krause, Daria Ezeriņa, Christian Ickes, Julian Wojtachnia, Christine S. Gibhardt, Magdalena Shumanska, Khadija Wahni, Andrea Paluschkiwitz, Julia Malo Pueyo, Ekaterina Baranova, Wim Vranken, Hedwig Stanisz, Ioana Stejerean‐Todoran, Michael P. Schön, Joris Messens, Ivan Bogeski   +17 more
wiley   +1 more source

Preparation, chemical structure, and immunostimulatory activity of a water-soluble heteropolysaccharide from Suillus granulatus fruiting bodies

Food Chemistry: X, 2022
A water-soluble heteropolysaccharide (SGP2-1) was purified from Suillus granulatus fruiting bodies by anion-exchange chromatography and gel permeation chromatography.
Xiong Gao, Ranhua Zeng, Chi-Tang Ho, Bin Li, Shaodan Chen, Chun Xiao, Huiping Hu, Manjun Cai, Zhongzheng Chen, Yizhen Xie, Qingping Wu   +10 more
doaj   +1 more source

Combining antibody conjugates with cytotoxic and immune‐stimulating payloads maximizes anti‐cancer activity

Molecular Oncology, EarlyView.
Methods to improve antibody–drug conjugate (ADC) treatment durability in cancer therapy are needed. We utilized ADCs and immune‐stimulating antibody conjugates (ISACs), which are made from two non‐competitive antibodies, to enhance the entry of toxic payloads into cancer cells and deliver immunostimulatory agents into immune cells.
Tiexin Wang, Dong Jun Koo, Peter M. Tessier, Greg M. Thurber   +3 more
wiley   +1 more source

Purification of Cortactin Using Anion Exchange Chromatography [PDF]

, 2014
Cortactin is an actin-binding protein that has been shown to be involved in cellular migration and metastases in cancer. Bacterially expressed and purified cortactin protein is often used in in vitro assays to examine cortactin’s role in promoting cell ...
Priester, Jacob
core   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Liquid-gel chromatography on lipophilic-hydrophobic Sephadex derivatives

Journal of Lipid Research, 1970
Hydrophobic long-chain alkyl ethers of Sephadex have been synthesized and tested for use in liquid chromatography. With columns prepared in such polar solvents as methanol, reversed-phase systems are obtained where compounds separate in order of ...
J. Ellingboe, E. Nyström, J. Sjövall
doaj   +1 more source

Studies on the chemical compositions of Hyptis suaveolens (L.) Poit [PDF]

Journal of the Serbian Chemical Society, 2019
This study aimed at identifying the chemical compounds isolated from the aerial parts and roots of Hyptis suaveolens (L.) Poit. The compounds were isolated and purified by a combination of various chromatographic techniques including silica gel column ...
Tang Gengqiu, Liu Xile, Gong Xue, Lin Xiaojing, Lai Xiudi, Wang Dong, Ji Shengguo   +6 more
doaj   +1 more source

Changes in zebrafish (Danio rerio) lens crystallin content during development. [PDF]

, 2013
PurposeThe roles that crystallin proteins play during lens development are not well understood. Similarities in the adult crystallin composition of mammalian and zebrafish lenses have made the latter a valuable model for examining lens function.
Corbin, Rebecca W, Ding, Linlin, Horwitz, Joseph, Posner, Mason, Wages, Phillip   +4 more
core   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source

Screening, Enrichment and Structural Identification of Bitter Almond Antibacterial Peptides

Shipin gongye ke-ji
To explore the preparation, enrichment, and structural analysis of bitter almond antimicrobial peptides, in this experiment, the protein was extracted from the defatted bitter almonds, and the protein was hydrolyzed by enzymes to prepare bitter almond ...
Tao ZHANG, Jie MA, Guofang NIAN, Zhiyi ZHOU, Ting ZHANG, Jianzhong ZHOU   +5 more
doaj   +1 more source