Results 11 to 20 of about 835,345 (308)

Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus [PDF]

, 2020
Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta ...
Drake, P, Ibrahim, AEC, Ma, J, Van Dolleweerd, CJ   +3 more
core   +1 more source

A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system

Virology Journal, 2020
Background Reverse genetics is used in many laboratories around the world and enables the creation of tailor-made influenza viruses with a desired genotype or phenotype.
Sushant Bhat, Dagmara Bialy, Joshua E. Sealy, Jean-Remy Sadeyen, Pengxiang Chang, Munir Iqbal   +5 more
doaj   +1 more source

Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system

Stem Cell Research & Therapy, 2019
Background Pigs have emerged as one of the most popular large animal models in biomedical research, which in many cases is considered as a superior choice over rodent models.
Junjun Xu, Leqian Yu, Jianxiong Guo, Jinzhu Xiang, Zheng Zheng, Dengfeng Gao, Bingbo Shi, Haiyang Hao, Deling Jiao, Liang Zhong, Yu Wang, Jun Wu, Hongjiang Wei, Jianyong Han   +13 more
doaj   +1 more source

Endogenous leptin promotes autophagy in EBSS-induced PFCs

Animal Cells and Systems, 2019
Leptin is an important adipokine and plays a vital role in animals. However, the role of leptin in the autophagic response of pig fibroblast cells (PFCs) has not been fully elucidated.
Deling Jiao, Zhen Yang, Lulu Wang, Binyue Hu, Jing Wang, Anyong Xu, Wenmin Cheng, Baoyu Jia, Yubo Qing, Hong-Ye Zhao, Hong-Jiang Wei   +10 more
doaj   +1 more source

Identification of mercury‐resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene

Indonesian Journal of Biotechnology, 2021
Streptomyces is one of mercury‐resistant bacteria which can convert Hg2+ into nontoxic Hg0 . This study aimed to identify mercury‐resistant Streptomyces present in the Cyperus rotundus rhizosphere from artisanal small‐scale gold mining (ASGM) area and ...
Wahyu Aristyaning Putri, Hanum Mukti Rahayu, Anis Uswatun Khasanah, Langkah Sembiring, Masashi Kawaichi, Yekti Asih Purwestri   +5 more
doaj   +1 more source

Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains [PDF]

, 2009
Background: Homologous recombination mediated by the lambda-Red genes is a common method for making chromosomal modifications in Escherichia coli.
Bingle, Lewis EH, Busby, Stephen J. W., Heurlier, Karin, Hobman, Jon L, Lee, David J, Pallen, Mark J., Penn, Charles W   +6 more
core   +5 more sources

Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy

AMB Express, 2017
The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation ...
Liting Cao, Yancheng Zhou, Lin Huang, Shiqi Dong, Yue Ma   +4 more
doaj   +1 more source

Sequence-specific protein aggregation generates defined protein knockdowns in plants [PDF]

, 2016
Protein aggregation is determined by short (5-15 amino acids) aggregation-prone regions (APRs) of the polypeptide sequence that self-associate in a specific manner to form beta-structured inclusions.
Aesaert, Stijn, Betti, Camilla, Coutuer, Silvie, De Rycke, Riet, De Smet, Frederik, Gallardo, Rodrigo, Inzé, Dirk, Mishev, Kiril, Rombaut, Debbie, Rousseau, Frederic, Russinova, Eugenia, Schymkowitz, Joost, Van Breusegem, Frank, Van Lijsebettens, Maria, Vanhoutte, Isabelle, Vuylsteke, Marnik, Xu, Jie   +16 more
core   +2 more sources

Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning

Frontiers in Bioengineering and Biotechnology, 2020
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Pu Yan, Yanjing Zeng, Wentao Shen, Decai Tuo, Xiaoying Li, Peng Zhou   +5 more
doaj   +1 more source

A qPCR Method to Distinguish between Expression of Transgenic and Endogenous Copies of Genes

Bio-Protocol, 2023
Study of gene function in eukaryotes frequently requires data on the impact of the gene when it is expressed as a transgene, such as in ectopic or overexpression studies. Currently, the use of transgenic constructs designed to achieve these aims is often
William Bezodis, Helen Prescott, Daniela Vlad, Hugh Dickinson   +3 more
doaj   +1 more source