Results 21 to 30 of about 835,345 (308)
Overlap extension cloning of PCR products into a Gateway-compatible plasmid vector
BioTechniques, 2023 A PCR cloning method that combines a dual selection pGATE-1 plasmid vector and an improved overlap extension cloning was developed. This efficient and cost-effective method allows for the introduction of DNA fragments into the Gateway cloning pipeline ...
Omid Zare-Mehrjerdi +2 more
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Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls [PDF]
, 2007 Arabidopsis cell walls contain large amounts of pectins and hemicelluloses, which are predominantly synthesized via the common precursor UDP-glucuronic acid.
Klinghammer, Michaela, Tenhaken, Raimund
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Plant Methods, 2009 Background The progress and completion of various plant genome sequencing projects has paved the way for diverse functional genomic studies that involve cloning, modification and subsequent expression of target genes. This requires flexible and efficient
Etchells J Peter +3 more
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Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
Nature Communications, 2021 Direct cloning of biosynthetic gene clusters is difficult due to their size and repetitive nature. Here, the authors present CAPTURE, which uses Cas12a and in vivo Cre-lox recombination for efficient cloning of gene clusters up to 113 kb, and demonstrate
Behnam Enghiad +7 more
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Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]
, 2015 Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas +3 more
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New Clox Systems for rapid and efficient gene disruption in Candida albicans [PDF]
, 2014 Acknowledgements: We are grateful to Janet Quinn, Lila Kastora, Joanna Potrykus, Michelle Leach, and others for sharing their experiences with the Clox cassettes.
Ballou, Elizabeth R +6 more
core +7 more sources
Transformation of the Methylotrophic Actinomycete Amycolatopis methanolica with Plasmid DNA: Stimulatory Effect of a pMEA300-Encoded Gene [PDF]
, 1995 Amycolatopsis methanolica contains a 13.29-kb plasmid (pMEA300) present both in the free state and integrated at a unique genomic location. A pMEA300-free derivative (strain WV1) was selected, allowing further analysis of pMEA300-encoded functions. Whole
Dijkhuizen, L., +2 more
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pXST, a novel vector for TA cloning and blunt-end cloning
BMC Biotechnology, 2018 Background With the rapid development of sequencing technologies, increasing amount of genomic information has been accumulated. To clone genes for further functional studies in large scale, a cheap, fast and efficient cloning vector is desired.
Qin Liu +7 more
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ABC cloning: An efficient, simple, and rapid restriction/ligase-free method
MethodsX, 2019 DNA cloning remains the primary step before the further investigation of gene function. Restriction enzyme-based cloning methods are still widely used and numerous restriction-free cloning techniques are available as alternatives.
Samir El Qaidi, Philip R. Hardwidge
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Journal of Dentistry Indonesia, 2015 DNA cloning is one of the most important techniques In the field of molecular biology, with a critical role in analyzing the structure and function of genes and their adjacent regulatory regions.
Elza Ibrahim Auerkari +2 more
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