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Group II intron-anchored gene deletion in Clostridium. [PDF]

PLoS ONE, 2011
Clostridium plays an important role in commercial and medical use, for which targeted gene deletion is difficult. We proposed an intron-anchored gene deletion approach for Clostridium, which combines the advantage of the group II intron "ClosTron" system
Kaizhi Jia, Yan Zhu, Yanping Zhang, Yin Li   +3 more
doaj   +3 more sources

Evaluation of Aeromonas hydrophila outer membrane protein deletion strains in response to environmental stresses

浙江大学学报. 农业与生命科学版, 2022
In order to evaluate systematically the biological functions of outer membrane proteins (OMPs) under environmental stresses, we selected Aeromonas hydrophila ATCC 7966 as the research object, and constructed 33 OMP deletion strains to determine their ...
LI Zeqi, WANG Yuqian, LI Xiaoyan, LIN Xiangmin, YUAN Jun   +4 more
doaj   +1 more source

Prognostic significance of copy number variation in B-cell acute lymphoblastic leukemia

Frontiers in Oncology, 2022
Copy number variations (CNVs) are widespread in both pediatric and adult cases of B-cell acute lymphoblastic leukemia (B-ALL); however, their clinical significance remains unclear.
Yang Song, Qiuyun Fang, Yingchang Mi
doaj   +1 more source

Towards the Understanding of the Function of Lanthipeptide and TOMM-Related Genes in Haloferax mediterranei

Biology, 2023
Research on secondary metabolites produced by Archaea such as ribosomally synthesized and post-translationally modified peptides (RiPPs) is limited. The genome of Haloferax mediterranei ATCC 33500 encodes lanthipeptide synthetases (medM1, medM2, and ...
Thales Costa, Elena Cassin, Catarina Moreirinha, Sónia Mendo, Tânia Sousa Caetano   +4 more
doaj   +1 more source

Deletion of the Neuronal Transcription Factor Satb1 Induced Disturbance of the Kinome and Mechanisms of Hypoxic Preconditioning

Biology, 2023
Genetic disorders affecting the functioning of the brain lead not only to the development of numerous hereditary diseases but also to the development of neurodegenerative and cognitive disorders.
Egor A. Turovsky, Viktor S. Tarabykin, Elena G. Varlamova   +2 more
doaj   +1 more source

Detecting Photoactivatable Cre-mediated Gene Deletion Efficiency in Escherichia coli

Bio-Protocol, 2023
Gene deletion is one of the standard approaches in genetics to investigate the roles and functions of target genes. However, the influence of gene deletion on cellular phenotypes is usually analyzed sometime after the gene deletion was introduced.
Yuta Koganezawa, Yuichi Wakamoto, Moritoshi Sato, Miki Umetani   +3 more
doaj   +1 more source

Comprehensive evaluation of the safety and immunogenicity of a gene-deleted variant pseudorabies virus attenuated vaccine

Veterinary Research, 2022
Pseudorabies virus (PRV) variant infections have caused a substantial economic impact on swine production in the absence of new powerful candidate vaccines.
Ling Li, Yongfeng Du, Yanbin Zhang, Pengyu Li, Xinyue Liu, Xin Zhang, Jing Li, Tong Zhang, Xin Li, Dong Xiao, Peng Liu, Peng Qi, Jin Xiao   +12 more
doaj   +1 more source

Establishment of High-Efficiency Screening System for Gene Deletion in Fusarium venenatum TB01

Journal of Fungi, 2022
Genetic engineering is one of the most effective methods to obtain fungus strains with desirable traits. However, in some filamentous fungi, targeted gene deletion transformant screening on primary transformation plates is time-consuming and laborious ...
Sheng Tong, Kexin An, Wenyuan Zhou, Wuxi Chen, Yuanxia Sun, Qinhong Wang, Demao Li   +6 more
doaj   +1 more source

srdA mutations suppress the rseA/cpsA deletion mutant conidiation defect in Aspergillus nidulans

Scientific Reports, 2023
Conidiation is an important reproductive process in Aspergillus. We previously reported, in A. nidulans, that the deletion of a putative glycosyltransferase gene, rseA/cpsA, causes an increase in the production of extracellular hydrolases and a severe ...
Masahiro Ogawa, Ryouichi Fukuda, Ryo Iwama, Yasuji Koyama, Hiroyuki Horiuchi   +4 more
doaj   +1 more source

Plasmodium falciparum pfhrp2 and pfhrp3 Gene Deletions from Persons with Symptomatic Malaria Infection in Ethiopia, Kenya, Madagascar, and Rwanda

Emerging Infectious Diseases, 2022
Histidine-rich protein 2 (HRP2)–based rapid diagnostic tests detect Plasmodium falciparum malaria and are used throughout sub-Saharan Africa. However, deletions in the pfhrp2 and related pfhrp3 (pfhrp2/3) genes threaten use of these tests.
Eric Rogier, Jessica N. McCaffery, Doug Nace, Samaly Souza Svigel, Ashenafi Assefa, Jimee Hwang, Simon Kariuki, Aaron M. Samuels, Nelli Westercamp, Arsène Ratsimbasoa, Milijaona Randrianarivelojosia, Aline Uwimana, Venkatachalam Udhayakumar, Eric S. Halsey   +13 more
doaj   +1 more source