Results 21 to 30 of about 2,666,439 (212)

Malaria Species And Southeast Asian Ovalocytosis Defined By A 27-BP Deletion In The Erythrocyte Band 3 Gene [PDF]

open access: yes, 2002
To evaluate the resistance of SAO against species specific malaria infection, relationships between parasite species and the 27-bp deletion in the band 3 gene were studied in malaria endemic Sumba Island, eastern Indonesia.
Soemantri, Augustinus   +2 more
core   +2 more sources

Detection of glutathione S-transferases M1, T1 gene deletions among cancer patients in Mongolia

open access: yesProceedings of the Mongolian Academy of Sciences, 2020
Various types of toxic xenobiotic and electrophilic compounds, which were formed from the glutathione S-transferases cell metabolism and the oxidation stress, are the group enzymes with detoxification roles that are involved in the metabolism phase II ...
Temuujin Janchiv   +3 more
doaj   +1 more source

Effects of Four Critical Gene Deletions in Saccharomyces cerevisiae on Fusel Alcohols during Red Wine Fermentation

open access: yesFermentation, 2023
Excessive fusel alcohols in red wine will bring an uncomfortable bitterness and generate an intoxicating effect, which affects the quality and attractivity of the red wine. In order to achieve better regulation of fusel alcohols in red wine, strains with
Tongshuai Yan   +4 more
doaj   +1 more source

Generation of gene deletions and gene replacements in Escherichia coli O157:H7 using a temperature sensitive allelic exchange system [PDF]

open access: yes, 2006
In this work we describe protocols for the generation of gene deletions and gene replacements using a temperature sensitive plasmid in Escherichia coli O157:H7.
James R. Emmerson   +6 more
core   +1 more source

Two novel Bartonella (sub)species isolated from edible dormice (Glis glis): hints of cultivation stress-induced genomic changes

open access: yesFrontiers in Microbiology, 2023
Bartonelloses are neglected emerging infectious diseases caused by facultatively intracellular bacteria transmitted between vertebrate hosts by various arthropod vectors. The highest diversity of Bartonella species has been identified in rodents.
Oldřich Bartoš   +15 more
doaj   +1 more source

Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing [PDF]

open access: yesGenomics & Informatics, 2018
Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis.
Minho Lee   +6 more
doaj   +1 more source

Biochemical and structural characterization of the apicoplast dihydrolipoamide dehydrogenase of Plasmodium falciparum

open access: yesBioscience Reports, 2015
PDC (pyruvate dehydrogenase complex) is a multi-enzyme complex comprising an E1 (pyruvate decarboxylase), an E2 (dihydrolipomide acetyltransferase) and an E3 (dihydrolipoamide dehydrogenase). PDC catalyses the decarboxylation of pyruvate and forms acetyl-
Larissa M. Laine   +3 more
doaj   +1 more source

Reconstitution of Mycobacterium marinum Nonhomologous DNA End Joining Pathway in Leishmania

open access: yesmSphere, 2022
In mammalian cells, DNA double-strand breaks (DSBs) are mainly repaired by nonhomologous end joining (NHEJ) pathway. Ku (a heterodimer formed by Ku70 and Ku80 proteins) and DNA ligase IV are the core NHEJ factors.
Wen-Wei Zhang   +3 more
doaj   +1 more source

A Traceless Selection: Counter-selection System That Allows Efficient Generation of Transposon and CRISPR-modified T-cell Products

open access: yesMolecular Therapy: Nucleic Acids, 2016
Recent years have seen major breakthroughs in genome-engineering systems, such as transposon-mediated gene delivery systems and CRISPR-Cas9-mediated genome-editing tools.
Riccardo Mezzadra   +3 more
doaj   +1 more source

Characterization and immunoprotection of thioredoxin reductase TrxB knockout mutant of Salmonella Enteritidis

open access: yesFrontiers in Cellular and Infection Microbiology
BackgroundSalmonella Enteritidis (S. Enteritidis) is an important zoonotic pathogen that poses a major threat to animals and human health. TrxB, as a key component of the thioredoxin system, is a thioredoxin reductase ubiquitously present in organisms ...
Siping Zhu   +9 more
doaj   +1 more source

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