Results 21 to 30 of about 838,531 (316)
Generation of gene deletions and gene replacements in Escherichia coli O157:H7 using a temperature sensitive allelic exchange system [PDF]
, 2006 In this work we describe protocols for the generation of gene deletions and gene replacements using a temperature sensitive plasmid in Escherichia coli O157:H7.
James R. Emmerson +6 more
core +1 more source
Detection of glutathione S-transferases M1, T1 gene deletions among cancer patients in Mongolia
Proceedings of the Mongolian Academy of Sciences, 2020 Various types of toxic xenobiotic and electrophilic compounds, which were formed from the glutathione S-transferases cell metabolism and the oxidation stress, are the group enzymes with detoxification roles that are involved in the metabolism phase II ...
Temuujin Janchiv +3 more
doaj +1 more source
Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing [PDF]
Genomics & Informatics, 2018 Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis.
Minho Lee +6 more
doaj +1 more source
Angiotensin-converting enzyme insertion/deletion polymorphism does not influence the restenosis rate after coronary stent implantation [PDF]
, 2002 Background. Experimental studies have shown an activation of the angiotensin-converting enzyme (ACE) system as a response to endothelial injury. Recent publications have elucidated the hypothesis that the ACE gene polymorphism may influence the level of ...
Hausmann, Dirk +10 more
core +1 more source
Reconstitution of Mycobacterium marinum Nonhomologous DNA End Joining Pathway in Leishmania
mSphere, 2022 In mammalian cells, DNA double-strand breaks (DSBs) are mainly repaired by nonhomologous end joining (NHEJ) pathway. Ku (a heterodimer formed by Ku70 and Ku80 proteins) and DNA ligase IV are the core NHEJ factors.
Wen-Wei Zhang +3 more
doaj +1 more source
Y-Chromosome Microdeletions: A Review of Prevalence, Screening, and Clinical Considerations
The Application of Clinical Genetics, 2021 Matthew J Rabinowitz, Phillip J Huffman, Nora M Haney, Taylor P Kohn The James Buchanan Brady Urological Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USACorrespondence: Taylor P KohnJames Buchanan Brady Urological Institute ...
Rabinowitz MJ +3 more
doaj
Molecular Therapy: Nucleic Acids, 2016 Recent years have seen major breakthroughs in genome-engineering systems, such as transposon-mediated gene delivery systems and CRISPR-Cas9-mediated genome-editing tools.
Riccardo Mezzadra +3 more
doaj +1 more source
Markerless Gene Deletion System for Sphingomonads [PDF]
Applied and Environmental Microbiology, 2012 ABSTRACT Here, we suggest that natural streptomycin resistance of many sphingomonads resides within rpsL . We constructed a dominant, streptomycin-sensitive rpsL allele and demonstrated its use as a counterselection marker in several sphingomonads.
Kaczmarczyk A +2 more
openaire +3 more sources
Frontiers in Cellular and Infection MicrobiologyBackgroundSalmonella Enteritidis (S. Enteritidis) is an important zoonotic pathogen that poses a major threat to animals and human health. TrxB, as a key component of the thioredoxin system, is a thioredoxin reductase ubiquitously present in organisms ...
Siping Zhu +9 more
doaj +1 more source
MycobiologyHelix-loop-helix (HLH) transcription factors are essential regulators of various cellular processes, including growth, differentiation, and development.
Mohammed A. Abdo-Elgabbar +5 more
doaj +1 more source