Results 61 to 70 of about 2,666,439 (212)

Rapid construction of mycobacterial mutagenesis vectors using ligation-independent cloning [PDF]

, 2010
Targeted mutagenesis is one of the major tools for determining the function of a given gene and its involvement in bacterial pathogenesis. In mycobacteria, gene deletion is often accomplished by using allelic exchange techniques that commonly utilise a ...
Neil G. Stoker, Stoker, N G, Francois-Xavier Chauviac, Balhana, R J C, Sikder, M H, Stoker, Neil G., Chauviac, Francois-Xavier, Sharon L. Kendall, Sikder, Mahmudul Hasan, Kendall, S L, Birkbeck College, Chauviac, F-X, Mahmudul Hasan Sikder, Kendall, Sharon L., Ricardo Balhana, Balhana, Ricardo   +15 more
core   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

NADPH Dehydrogenase Gene Regulates Energy Distribution and Fatty Acid Metabolism During Fruiting Body Formation in the Filamentous Fungus Podospora anserina

Microbial Biotechnology
Nicotinamide adenine dinucleotide phosphate (NADPH) dehydrogenase is an oxidoreductase involved in many physiological processes and metabolic pathways. However, its role in filamentous fungal physiology is still unclear.
Yanling Qiu, Amechi S. Nwankwegu, Yue Sun, Yuanjing Li, Weizhao Chen, Gang Liu, Ning Xie   +6 more
doaj   +1 more source

Copy number variation screen identifies a rare de novo deletion at chromosome 15q13.1-13.3 in a child with language impairment [PDF]

, 2015
A significant proportion of children (up to 7% in the UK) present with pronounced language difficulties that cannot be explained by obvious causes like other neurological and medical conditions.
Dianne F Newbury, Paracchini, Silvia, Emily Reeves, Charles Hulme, Pettigrew, Kerry A., Hayiou-Thomas, Marianna E., Margaret J Snowling, Simpson, Nuala H., Thompson, Paul A., Paul Thompson, Margaret J. Snowling (782135), Marianna E Hayiou-Thomas, Marianna E. Hayiou-Thomas (782131), Nuala H Simpson, Anahita Sharma, Angela Martinelli (782133), Thompson, Paul; id_orcid, Thompson, Paul, Hulme, Charles, Charles Hulme (782134), Snowling, Margaret J., Ruth Leavett, Silvia Paracchini, Kerry A. Pettigrew (782128), Anahita Sharma (782132), Silvia Paracchini (238619), Emily Reeves (782129), Kerry A Pettigrew, Angela Martinelli, Martinelli, Angela, Newbury, Dianne F., Sharma, Anahita, Ruth Leavett (782130), Dianne F. Newbury (297003), Leavett, Ruth, Paul Thompson (515751), Reeves, Emily, Nuala H. Simpson (703485)   +37 more
core   +1 more source

Translophagy—A potential link between autophagy impairment and translational errors

FEBS Letters, EarlyView.
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk, Gailing Ma, Robin Antrobus, Diego Manni, Marco Trevisan‐Herraz, Mustafa Khwaja, Max Brown, Gavin D. Richardson, Masaaki Komatsu, Daniel Erskine, Viktor I. Korolchuk, Tetsushi Kataura   +11 more
wiley   +1 more source

Additional Insertion of gC Gene Triggers Better Immune Efficacy of TK/gI/gE-Deleted Pseudorabies Virus in Mice

Viruses
In recent years, pseudorabies virus (PRV) variants have resulted in an epidemic in swine herds and huge economic losses in China. Therefore, it is essential to develop an efficacious vaccine against the spread of PRV variants.
Zhuoyun Wu, Jiahuan Deng, Meijing Chen, Peiqi Lu, Zhibin Yan, Xiaoyan Wu, Qiuyun Ji, Huiying Fan, Yongwen Luo, Chunmei Ju   +9 more
doaj   +1 more source

Consequences of CYP2D6 Copy-Number Variation for Pharmacogenomics in Psychiatry

Frontiers in Psychiatry, 2019
Pharmacogenomics represents a potentially powerful enhancement to the current standard of care for psychiatric patients. However, a variety of biological and technical challenges must be addressed in order to provide adequate clinical decision support ...
Joseph P. Jarvis, Arul Prakasam Peter, Jeffrey A. Shaman   +2 more
doaj   +1 more source

Functional analysis of the magnetosome island in Magnetospirillum gryphiswaldense: the mamAB operon is sufficient for magnetite biomineralization [PDF]

, 2011
Bacterial magnetosomes are membrane-enveloped, nanometer-sized crystals of magnetite, which serve for magnetotactic navigation. All genes implicated in the synthesis of these organelles are located in a conserved genomic magnetosome island (MAI).
Voigt Birgit, Losse, A., Richter, M., Katzmann, E., Lohße Anna, Michael Richter, Richter, Michael, Emanuel Katzmann, Ullrich, Susanne, Birgit Voigt, Wanner, G., Katzmann, Emanuel, Borg, Sarah, Lohsse, Anna, Gerd Wanner, Ullrich, S., Wanner Gerd, Ullrich Susanne, Schweder, Thomas, Voigt, Birgit, Dirk Schüler, Schweder Thomas, Richter Michael, Sarah Borg, Wanner, Gerd, Susanne Ullrich, Borg Sarah, Schweder, T., Schüler, Dirk, Schüler Dirk, Voigt, B., Katzmann Emanuel, Borg, S., Thomas Schweder, Schueler, D., Anna Lohsse   +35 more
core   +2 more sources

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

FEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

A Simple and Universal System for Gene Manipulation in Aspergillus fumigatus: In Vitro-Assembled Cas9-Guide RNA Ribonucleoproteins Coupled with Microhomology Repair Templates

mSphere, 2017
CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 is a novel genome-editing system that has been successfully established in Aspergillus fumigatus.
Qusai Al Abdallah, Wenbo Ge, Jarrod R. Fortwendel   +2 more
doaj   +1 more source