Results 251 to 260 of about 4,269,896 (304)
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Cancer gene therapy delivery delivery delivery
Frontiers in Bioscience, 2002Gene therapy for cancer treatment represents a promising approach that has shown selectivity and efficacy in experimental systems as well as clinical trials. Some major problems remain to be solved before this strategy becomes routinely adopted in the clinic, one of the main challenges being the improvement of gene delivery.
Olga, Greco +3 more
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Advanced Drug Delivery Reviews, 2001
Efficient non-viral gene delivery based on cationic polymers as DNA-condensing agents is dependent on a variety of factors, e.g. complex size, complex stability, toxicity, immunogenicity, protection against DNase degradation, and intracellular trafficking and processing of the DNA.
Gerrit Borchard
exaly +3 more sources
Efficient non-viral gene delivery based on cationic polymers as DNA-condensing agents is dependent on a variety of factors, e.g. complex size, complex stability, toxicity, immunogenicity, protection against DNase degradation, and intracellular trafficking and processing of the DNA.
Gerrit Borchard
exaly +3 more sources
Nanoparticles for Gene Delivery
Small, 2013AbstractNanocarriers are a new type of nonviral gene carriers, many of which have demonstrated a broad range of pharmacological and biological properties, such as being biodegradable in the body, stimulus‐responsive towards the surrounding environment, and an abiltiy to specifically targeting certain disease sites.
Huayu, Tian, Jie, Chen, Xuesi, Chen
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Current Protocols in Human Genetics, 1999
AbstractThis unit describes generation of and gene transfer to several commonly used airway models. Isolation and transduction of primary airway epithelial cells are first described. Next, the preparation of polarized airway epithelial monolayers is outlined. Transduction of these polarized cells is also described.
D, Duan, Y, Zhang, J F, Engelhardt
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AbstractThis unit describes generation of and gene transfer to several commonly used airway models. Isolation and transduction of primary airway epithelial cells are first described. Next, the preparation of polarized airway epithelial monolayers is outlined. Transduction of these polarized cells is also described.
D, Duan, Y, Zhang, J F, Engelhardt
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2008
Gene delivery by retroviruses is an easy and safe tool to stably over express a gene of interest and determine its role in a cell model. The gene of interest is cloned into the multiple cloning site of a retroviral vector that also contains a packaging signal and an antibiotic resistance marker for selection.
Valerie, Deregowski, Ernesto, Canalis
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Gene delivery by retroviruses is an easy and safe tool to stably over express a gene of interest and determine its role in a cell model. The gene of interest is cloned into the multiple cloning site of a retroviral vector that also contains a packaging signal and an antibiotic resistance marker for selection.
Valerie, Deregowski, Ernesto, Canalis
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Journal of Dermatological Science, 2008
Over the past decade, many approaches to transferring genes into the skin have been investigated. However, most such approaches have been specifically aimed against genodermatosis, and have not produced sufficient results. The goal of such research is to develop a method in which genes are transferred easily, efficiently and stably into keratinocytes ...
Yasushi, Kikuchi +2 more
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Over the past decade, many approaches to transferring genes into the skin have been investigated. However, most such approaches have been specifically aimed against genodermatosis, and have not produced sufficient results. The goal of such research is to develop a method in which genes are transferred easily, efficiently and stably into keratinocytes ...
Yasushi, Kikuchi +2 more
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Journal of Controlled Release
Gene delivery has revolutionized conventional medical approaches to vaccination, cancer, and autoimmune diseases. However, current gene delivery methods are limited to either intravenous administration or direct local injections, failing to achieve well biosafety, tissue targeting, drug retention, and transfection efficiency for desired therapeutic ...
Wentao, Zhang +5 more
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Gene delivery has revolutionized conventional medical approaches to vaccination, cancer, and autoimmune diseases. However, current gene delivery methods are limited to either intravenous administration or direct local injections, failing to achieve well biosafety, tissue targeting, drug retention, and transfection efficiency for desired therapeutic ...
Wentao, Zhang +5 more
openaire +2 more sources
Current Protocols in Human Genetics, 2001
AbstractThe delivery of genes to skeletal muscle by myoblast implantation, DNA injection, or viral transduction has therapeutic applications for human neuromuscular and systemic disorders, many of which are now represented by transgenic or “knockout” mouse models.
Matthew L, Springer +2 more
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AbstractThe delivery of genes to skeletal muscle by myoblast implantation, DNA injection, or viral transduction has therapeutic applications for human neuromuscular and systemic disorders, many of which are now represented by transgenic or “knockout” mouse models.
Matthew L, Springer +2 more
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2008
Adenoviruses have a number advantages as gene delivery vectors, including ability to transduce a wide variety of non-dividing and dividing cells with high efficiency, relative ease of construction, and ability to be purified as high-titer viral stocks. These characteristics make adenoviruses particularly attractive for over-expressing specific genes in
Renny T, Franceschi, Chunxi, Ge
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Adenoviruses have a number advantages as gene delivery vectors, including ability to transduce a wide variety of non-dividing and dividing cells with high efficiency, relative ease of construction, and ability to be purified as high-titer viral stocks. These characteristics make adenoviruses particularly attractive for over-expressing specific genes in
Renny T, Franceschi, Chunxi, Ge
openaire +2 more sources