Results 111 to 120 of about 3,123,960 (258)
Gene-knockout mice in malaria research: Useful or misleading?
, 2013 Gene-knockout mice have been extensively used in the study of several malaria-induced pathologies. Some investigators believe that the deficient, infected mice mimic disease aspects produced in the absence of the target gene, but others believe that the ...
Hernández Valladares, M. +2 more
core +1 more source
Medal commemorating Fiesta 2019 from Knockout Sports Bar in San Antonio. The obverse is shaped in the form of a sugar skull. Details on the skull include the skyline of San Antonio, two soccer balls, a football, boxing gloves, and text reading "Knockout
core
Effects of anti-inflammatory cytokine gene knockout (−/−) in mice.
, 2012 Effects of anti-inflammatory cytokine gene knockout (−/−) in mice.
Andrew Frisch (177002) +11 more
core +1 more source
Molecular Oncology, EarlyView.Combining osimertinib with the STING agonist ADU‐S100 activates innate and adaptive immunity to overcome the non‐inflamed microenvironment of Egfr‐mutant lung cancer. This combination increases NK and CD8+ T‐cell infiltration, associated with activation of the STING‐IRF3 pathway and local immunogenic cell death.
Jun Nishimura +19 more
wiley +1 more source
Gene targets and primers for CRISPR/Cas9 mediated knockout studies.
, 2015 Gene targets and primers for CRISPR/Cas9 mediated knockout studies.
Mika Rämet (133146) +11 more
core +1 more source
Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining
Molecular Oncology, EarlyView.IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis +3 more
wiley +1 more source
Escaping from CRISPR–Cas-mediated knockout: the facts, mechanisms, and applications
Cellular & Molecular Biology LettersClustered regularly interspaced short palindromic repeats and associated Cas protein (CRISPR–Cas), a powerful genome editing tool, has revolutionized gene function investigation and exhibits huge potential for clinical applications.
Ying Wang +5 more
doaj +1 more source
A Simple and Low-Cost CRISPR/Cas9 Knockout System Widely Applicable to Insects
InsectsThe CRISPR/Cas9 gene-editing system is a standard technique in functional genomics, with widespread applications. However, the establishment of a CRISPR/Cas9 system is challenging. Previous studies have presented numerous methodologies for establishing a
Jun Cao +5 more
doaj +1 more source
Molecular Oncology, EarlyView.We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober +16 more
wiley +1 more source
Finding novel vulnerabilities of hypomorphic BRCA1 alleles
Molecular Oncology, EarlyView.Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder +10 more
wiley +1 more source