Results 91 to 100 of about 3,123,960 (258)

Guidelines for optimized gene knockout using CRISPR/Cas9

BioTechniques, 2019
CRISPR/Cas9 technology has evolved as the most powerful approach to generate genetic models both for fundamental and preclinical research. Despite its apparent simplicity, the outcome of a genome-editing experiment can be substantially impacted by ...
Claude Van Campenhout, Pauline Cabochette, Anne-Clémence Veillard, Miklos Laczik, Agnieszka Zelisko-Schmidt, Céline Sabatel, Maxime Dhainaut, Benoit Vanhollebeke, Cyril Gueydan, Véronique Kruys   +9 more
doaj   +1 more source

Construction of a GLUT-1 and HIF-1α gene knockout cell model in HEp-2 cells using the CRISPR/Cas9 technique

, 2019
Zhong-Jie Lu,1 Qi Yu,2 Shui-Hong Zhou,2 Jun Fan,3 Li-Fang Shen,2 Yang-Yang Bao,2 Ting-Ting Wu,2 Min-Li Zhou,2 Ya-Ping Huang3 1Department of Radiotherapy, 2Department of Otolaryngology, 3State Key Laboratory for Diagnosis and Treatment of Infectious ...
Zhou ML, Shen LF, Lu ZJ, Fan J, Yu Q, Bao YY, Zhou SH, Huang YP, Wu TT   +8 more
core  

Delivery of human apolipoprotein (apo) E to liver by an [E1(-), E3(-), polymerase(-), pTP(-)] adenovirus vector containing a liver-specific promoter inhibits atherogenesis in immunocompetent apoE-deficient mice [PDF]

, 2006
Recombinant adenovirus (rAd)-mediated apoE gene transfer to the liver of apoE(-/-) mice is anti-atherogenic. However, first generation rAd vectors were associated with immune clearance of transduced hepatocytes, while an improved [E1(-), E3(-) polymerase(
Amalfitano, A, Owen, JS, Dickson, G, Graham, IR, Harris, JD   +4 more
core  

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Double-CRISPR Knockout Simulation (DKOsim): A Monte-Carlo randomization system to model cell growth behavior and infer the optimal library design for growth-based double knockout screens.

PLoS Computational Biology
Advances in functional genomic technology, notably CRISPR using Cas9 or Cas12, now allow for large-scale double perturbation screens in which pairs of genes are inactivated, allowing for the experimental detection of genetic interactions (GIs).
Yue Gu, Traver Hart, Luis Leon-Novelo, John Paul Shen   +3 more
doaj   +1 more source

Differential gene expression in Ndph knockout mice in retinal development [PDF]

, 2009
PURPOSE. Mutations in the NDP gene impair angiogenesis in the eyes of patients diagnosed with a type of blindness belonging to the group of exudative vitreoretinopathies. With this study, we aimed to investigate differential gene expression caused by the
Berger, W, Feil, S, Schäfer, N F, Luhmann, U F O   +3 more
core   +1 more source

Translophagy—A potential link between autophagy impairment and translational errors

FEBS Letters, EarlyView.
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk, Gailing Ma, Robin Antrobus, Diego Manni, Marco Trevisan‐Herraz, Mustafa Khwaja, Max Brown, Gavin D. Richardson, Masaaki Komatsu, Daniel Erskine, Viktor I. Korolchuk, Tetsushi Kataura   +11 more
wiley   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Genome-Wide Gene Expression Analysis in Response to Organophosphorus Pesticide Chlorpyrifos and Diazion in C.Elegans [PDF]

, 2010
pesticides (OPs) were originally designed to affect the nervous system by inhibiting the enzyme acetylcholinesterase, an important regulator of the neurotransmitter acetylcholine.
Kammenga Jan E., Joost A. G. Riksen, Riksen Joost A. G., Ana Viñuela, L Basten Snoek, Viñuela Ana, Jan E. Kammenga, Joost A G Riksen, Kammenga, J.E., Snoek, L.B., L. Basten Snoek, Viñuela Rodriguez, A., Jan E Kammenga, Snoek L. Basten, Riksen, J.A.G.   +14 more
core   +1 more source