Results 81 to 90 of about 3,123,960 (258)

Template plasmids optimized for deletion of multiple genes in yeast Saccharomyces cerevisiae

Microbiology Spectrum
For Saccharomyces cerevisiae, gene knockout is routinely performed by transformation with a linear DNA cassette consisting of a selection marker gene flanked by upstream and downstream sequences homologous to a target gene.
Yi-He Feng, Jing-Zhen Song, Jing Zhu, Zhiping Xie   +3 more
doaj   +1 more source

Design, construction, and analysis of specific zinc finger nucleases for microphthalmia - associate transcription factor

Brazilian Archives of Biology and Technology, 2012
This work studied the design, construction, and cleavage analysis of zinc finger nucleases (ZFNs) that could cut the specific sequences within microphthalmia - associate transcription factor (mitfa) of zebra fish.
Wenwen Wang, Hongmao Liu, Haibin Zhao, Zaili Luo, Yongxiang Shi   +4 more
doaj   +1 more source

Verification of CLTC gene knockout in genetic and protein levels.

, 2023
A Verification of CLTC gene knockout by western blotting analysis using sgRNA control, CLTC knockout and CLTC knockdown cells. β-actin was used as the loading control. B Verification of CLTC gene knockout by Immunofluorescence assay.
Jiajia Li (184267), Xueqi Wang (128278), Mifang Liang (158706), Tiezhu Liu (4175812), Qin Wang (109812), Shiwen Wang (132242), Wei Wu (27708), Dexin Li (158705), Aqian Li (121468), Xiaoxia Huang (1437292), Chuan Li (158692), Tao Huang (110613)   +11 more
core   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Construction of a gene knockout cassette for leucosporidium antarcticum [PDF]

, 2011
Leucosporidium .antarcticum, a psychrophilic yeast was isolated from Antarctica. It grew optimally at 12°C, and adapted well to the cold temperature in Antarctic. The whole genome of the L. antarcticum has been sequenced using the 454 system.
Koh, Joseph Soon Peng
core   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

The journey from gene knockout to clinical medicine: telotristat and sotagliflozin

, 2019
Marc S Rendell1,2 1Association of Diabetes Investigators, Omaha, NE 68131, USA; 2Rose Salter Medical Research Foundation, Newport Coast, CA 92657, USA Abstract: Gene knockout has been a powerful technique to evaluate the physiologic role of selected ...
Rendell MS
core  

Epigenetic reprogramming of lineage switching in cancer

FEBS Letters, EarlyView.
Cancer cells rarely commit to a single identity. Epigenetic mechanisms and tumor microenvironment cues push epithelial cells toward flexible, hybrid states that can shift into mesenchymal, neuroendocrine, or stem‐like fates, driving metastasis, drug resistance, and tumor heterogeneity. Targeting the epigenetic regulators behind these transitions, using
Ezgi Boyvatlı, Burcu Akman, Ezgi Bağırsakçı, Nese Atabey, Serap Erkek‐Ozhan   +4 more
wiley   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source