Results 121 to 130 of about 3,123,960 (258)

CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris

Synthetic and Systems Biotechnology
The important methylotrophic yeast Pichia pastoris has been utilized for the production of a variety of heterologous recombinant proteins and has great potential for use in the production of value-added compounds using methanol as a substrate.
Kaidi Chen, Gulikezi Maimaitirexiati, Qiannan Zhang, Yi Li, Xiangjian Liu, Hongting Tang, Xiang Gao, Bo Wang, Tao Yu, Shuyuan Guo   +9 more
doaj   +1 more source

Targeted gene knockout in chickens mediated by TALENs

, 2014
Genetically modified animals are used for industrial applications as well as scientific research, and studies on these animals contribute to a better understanding of biological mechanisms.
Lee, Hong Jo, Kim, Ki Hyun, Kim, Jin-Soo, Park, Tae Sub, Han, Jae Yong   +4 more
core   +1 more source

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose‐driven glioblastoma

Molecular Oncology, EarlyView.
The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka, Wioleta Cieślik, Wojciech Płaziński, Katarzyna Stępnik, Anna Boguszewska‐Czubara, Elżbieta Kot, Robert Musioł, Mateusz Jasica, Anna Mrozek‐Wilczkiewicz, Katarzyna Malarz   +9 more
wiley   +1 more source

A rice gene activation/knockout mutant resource for high throughput functional genomics

, 2012
[[abstract]]Using transfer DNA (T-DNA) with functions of gene trap and gene knockout and activation tagging, a mutant population containing 55,000 lines was generated.
;Hsing, Yue-Ie;Chern, Chyr-Guan;范宗宸;Fan, Ming-Jen;Lu, Po-Chang;Chen, Ku-Ting;Lo, Shuen-Fang;Sun, Peng-Kai;Ho, Shin-Lon;Lee, Kuo-Wei;Wang, Yi-Chieh;Huang, Wen–Lii;Ko, Swee-Suak;Chen, Shu;Chen, Jyh-Long;Chung, Chun-I;Lin, Yao-Cheng;Hour, Ai-Ling;Wang, Yet-Walt;Chang, Ya-Chi;Tsai, Min-Wei;Lin, Yi-Show;Chen, Yin-Chin;Yen, Hsing-Mu;Li, Charng-Pei;Wey, Chiu-Kai;Tseng, Ching-Shan;Lai, Ming-Hsing;Huang, Sheng-Chung;Chen, Liang-Jwu;Yu, Su-May
core  

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

Some blood biochemical indices of mice with pttg gene knockout

, 2011
Liver injury of any etiology is accompanied by increased fibrogenesis. One of the key genes that may influence in the development of liver fibrosis, is pttg gene (Pituitary tumor transforming gene).
N. O. Sybirna, S. V. Afanasyev, Ye. Z. Filyak, O. P. Kanyuka   +3 more
core   +1 more source

Circulating microRNA signatures of cachexia and cancer in Canis familiaris as a comparative oncology model for human disease

Molecular Oncology, EarlyView.
Circulating microRNAs as biomarkers of cachexia and sex‐specific cancer in senior dogs. In 25 client‐owned dogs, four circulating miRNAs (miR‐15a, miR‐15b, miR‐16, miR‐140) were downregulated in cachexia, with miR‐16 the strongest individual biomarker (AUC = 0.899).
Soon‐Seok Park, Kyongman An, Gyeonghwa Kim, Soo‐Nyun Choi, Hong‐Ki Lee, Keun Hur, Kyu‐Shik Jeong   +6 more
wiley   +1 more source

Stable knockout ofNRF2 gene in human A549 lung cancer cells by CRISPR/Cas9 system and its functional research

Zhongguo aizheng zazhi, 2019
Background and purpose: CNC-bZIP is a leucine zipper structure near the carboxyl terminal of homo sapiens nuclear factor (erythroid-derived 2)-like 2 (NRF2), which is necessary for DNA binding, and associated with NRF2 dimerization partners and the small
GONG Meiling, ZHANG Linlin, ZHENG Cuixia
doaj