BioTechniques, 2018 Here, we modified the multiple cloning sites from commonly used expression vectors to create a new suite of cloning plasmids that simplify and speed up cloning procedures in Escherichia coli.
Bettina Rohweder +3 more
doaj +1 more source
CRISPR/Cas9 high-throughput screening in cancer research [PDF]
E3S Web of Conferences, 2020 In recent years, CRISPR/Cas9 technology has developed rapidly. With its accurate, fast, and simple editing functions that can achieve gene activation, interference, knockout, and knock-in, it has become a powerful genetic screening tool that is widely ...
Liu Zhuoxin
doaj +1 more source
Organization and sequence of the gene encoding the human acrosin-trypsin inhibitor (HUSI-II) [PDF]
, 1993 A complete cDNA encoding the acrosin-trypsin inhibitor, HUSI-II, was used as a probe to isolate genomic clones from a human placenta library. Three clones which cover the entire HUSI-II gene were isolated and characterized.
Abelson +28 more
core +1 more source
Adventures in the enormous: a 1.8 million clone BAC library for the 21.7 Gb genome of loblolly pine. [PDF]
PLoS ONE, 2011 Loblolly pine (LP; Pinus taeda L.) is the most economically important tree in the U.S. and a cornerstone species in southeastern forests. However, genomics research on LP and other conifers has lagged behind studies on flowering plants due, in part, to ...
Zenaida V Magbanua +8 more
doaj +1 more source
RnaseIII and T4 Polynucleotide Kinase Sequence Biases and Solutions During RNA-Seq Library Construction [PDF]
, 2013 Background: RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement.
Changhoon Lee +5 more
core +3 more sources
Short-hairpin RNA library: identification of therapeutic partners for gefitinib-resistant non-small cell lung cancer. [PDF]
, 2014 Somatic mutations of the epidermal growth factor receptor often cause resistance to therapy with tyrosine kinase inhibitor in non-small cell lung cancer (NSCLC). In this study, we aimed to identify partner drugs and pathways that can induce cell death in
Koeffler, H Phillip +5 more
core +2 more sources
Development of a selection system for the detection of L-ribose isomerase expressing mutants of Escherichia coli [PDF]
, 2007 L-Arabinose isomerase (E.C. 5.3.1.14) catalyzes the reversible isomerization between L-arabinose and L-ribulose and is highly selective towards L-arabinose.
Beauprez, Joeri +7 more
core +1 more source
Analysis of shuffled gene libraries
Journal of Molecular Biology, 2002 In vitro recombination of homologous genes (family shuffling) has been proposed as an effective search strategy for laboratory evolution of genes and proteins. Few data are available, however, on the composition of shuffled gene libraries, from which one could assess the efficiency of recombination and optimize protocols.
Joern, John M +2 more
openaire +3 more sources
Preparation of trinucleotide phosphoramidites as synthons for the synthesis of gene libraries
Beilstein Journal of Organic Chemistry, 2018 The preparation of protein libraries is a key issue in protein engineering and biotechnology. Such libraries can be prepared by a variety of methods, starting from the respective gene library.
Ruth Suchsland +2 more
doaj +1 more source
htsint: a Python library for sequencing pipelines that combines data through gene set generation [PDF]
, 2015 Background: Sequencing technologies provide a wealth of details in terms of genes, expression, splice variants, polymorphisms, and other features. A standard for sequencing analysis pipelines is to put genomic or transcriptomic features into a context of
Bonneaud, Camille +2 more
core +3 more sources