A high-throughput method for Illumina RNA-Seq library preparation [PDF]
Frontiers in Plant Science, 2012 With the introduction of cost effective, rapid and superior quality next generation sequencing (NGS) techniques, gene expression analysis has become viable for labs conducting small projects as well as large-scale gene expression analysis experiments ...
Ravi eKumar +8 more
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The COVID-19 Drug and Gene Set Library [PDF]
Patterns, 2020 Summary: In a short period, many research publications that report sets of experimentally validated drugs as potential COVID-19 therapies have emerged.
Maxim V. Kuleshov +13 more
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Creating E.coli Library of Diagnostic Gene for Influenza B Virus Using Molecular Cloning Technique [PDF]
, 2014 Influenza viruses cause seasonal epidemics associated with high morbidity, especially in immunocompromised and extreme age group individuals. Many strategies for combating these viruses are in practice; however, developing and maintaining sensitive ...
Chinbat, Oyunsuvd
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A Robust and Versatile Method of Combinatorial Chemical Synthesis of Gene Libraries via Hierarchical Assembly of Partially Randomized Modules. [PDF]
PLoS ONE, 2015 A major challenge in gene library generation is to guarantee a large functional size and diversity that significantly increases the chances of selecting different functional protein variants.
Blagovesta Popova +4 more
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Adventures in the enormous: a 1.8 million clone BAC library for the 21.7 Gb genome of loblolly pine. [PDF]
PLoS ONE, 2011 Loblolly pine (LP; Pinus taeda L.) is the most economically important tree in the U.S. and a cornerstone species in southeastern forests. However, genomics research on LP and other conifers has lagged behind studies on flowering plants due, in part, to ...
Zenaida V Magbanua +8 more
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RNAi as a Tool to Study Virulence in the Pathogenic Yeast Candida glabrata
Frontiers in Microbiology, 2019 The yeast Candida glabrata is a major opportunistic pathogen causing mucosal and systemic infections in humans. Systemic infections caused by this yeast have high mortality rates and are difficult to treat due to this yeast’s intrinsic and frequently ...
Olena P. Ishchuk +19 more
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Solid Phase Assembly of Fully Protected Trinucleotide Building Blocks for Codon-Based Gene Synthesis
Applied Sciences, 2019 The use of pre-formed trinucleotides, representing codons of the 20 canonical amino acids, for oligonucleotide-directed mutagenesis offers the advantage of controlled randomization and generation of “smart libraries”. We here present a method
Ruth Suchsland +3 more
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CRISPR/Cas9 high-throughput screening in cancer research [PDF]
E3S Web of Conferences, 2020 In recent years, CRISPR/Cas9 technology has developed rapidly. With its accurate, fast, and simple editing functions that can achieve gene activation, interference, knockout, and knock-in, it has become a powerful genetic screening tool that is widely ...
Liu Zhuoxin
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Cloning and expression of porcine β1,4 N-acetylgalactosaminyl transferase encoding a new xenoreactive antigen [PDF]
, 2014 Xenograft rejection of pigs organs with an engineered mutation in the GGTA-1 gene (GTKO) remains a predominantly antibody mediated process which is directed to a variety of non-Gal protein and carbohydrate antigens.
Byrne, GW +4 more
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BioTechniques, 2018 Here, we modified the multiple cloning sites from commonly used expression vectors to create a new suite of cloning plasmids that simplify and speed up cloning procedures in Escherichia coli.
Bettina Rohweder +3 more
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