Results 1 to 10 of about 1,268,532 (356)

A high-throughput method for Illumina RNA-Seq library preparation [PDF]

Frontiers in Plant Science, 2012
With the introduction of cost effective, rapid and superior quality next generation sequencing (NGS) techniques, gene expression analysis has become viable for labs conducting small projects as well as large-scale gene expression analysis experiments ...
Ravi eKumar, Yasunori eIchihashi, Seisuke eKimura, Seisuke eKimura, Daniel H Chitwood, Lauren R Headland, Jie ePeng, Julin N Maloof, Neelima R Sinha   +8 more
doaj   +4 more sources

The COVID-19 Drug and Gene Set Library [PDF]

Patterns, 2020
Summary: In a short period, many research publications that report sets of experimentally validated drugs as potential COVID-19 therapies have emerged.
Maxim V. Kuleshov, Daniel J. Stein, Daniel J.B. Clarke, Eryk Kropiwnicki, Kathleen M. Jagodnik, Alon Bartal, John E. Evangelista, Jason Hom, Minxuan Cheng, Allison Bailey, Abigail Zhou, Laura B. Ferguson, Alexander Lachmann, Avi Ma'ayan   +13 more
doaj   +5 more sources

A gene-specific T2A-GAL4 library for Drosophila

eLife, 2018
We generated a library of ~1000 Drosophila stocks in which we inserted a construct in the intron of genes allowing expression of GAL4 under control of endogenous promoters while arresting transcription with a polyadenylation signal 3’ of the GAL4.
Pei-Tseng Lee, Jonathan Zirin, Oguz Kanca, Wen-Wen Lin, Karen L Schulze, David Li-Kroeger, Rong Tao, Colby Devereaux, Yanhui Hu, Verena Chung, Ying Fang, Yuchun He, Hongling Pan, Ming Ge, Zhongyuan Zuo, Benjamin E Housden, Stephanie E Mohr, Shinya Yamamoto, Robert W Levis, Allan C Spradling, Norbert Perrimon, Hugo J Bellen   +21 more
doaj   +4 more sources

The Comparison of Gene Expression from Multiple cDNA Libraries [PDF]

Genome Research, 2000
We describe a method for comparing the abundance of gene transcripts in cDNA libraries. This method allows for the comparison of gene expression in any number of libraries, in a single statistical analysis, to identify differentially expressed genes. Such genes may be of potential biological or pharmaceutical relevance.
Dov J. Stekel
  +6 more sources

Preparation of trinucleotide phosphoramidites as synthons for the synthesis of gene libraries [PDF]

Beilstein Journal of Organic Chemistry, 2018
The preparation of protein libraries is a key issue in protein engineering and biotechnology. Such libraries can be prepared by a variety of methods, starting from the respective gene library.
Ruth Suchsland, Bettina Appel, Sabine Müller   +2 more
doaj   +4 more sources

Model-based clustering with data correction for removing artifacts in gene expression data [PDF]

arXiv, 2016
The NIH Library of Integrated Network-based Cellular Signatures (LINCS) contains gene expression data from over a million experiments, using Luminex Bead technology. Only 500 colors are used to measure the expression levels of the 1,000 landmark genes measured, and the data for the resulting pairs of genes are deconvolved.
Raftery, Adrian E., Yeung, Ka Yee, Young, William Chad   +2 more
arxiv   +3 more sources

Non-parametric Bayesian modelling of digital gene expression data [PDF]

J Comput Sci Syst Biol 7:001-009 (2013), 2013
Next-generation sequencing technologies provide a revolutionary tool for generating gene expression data. Starting with a fixed RNA sample, they construct a library of millions of differentially abundant short sequence tags or "reads", which constitute a fundamentally discrete measure of the level of gene expression.
Gough, Julian, Vavoulis, Dimitrios V.
arxiv   +4 more sources

Wide-Scale Analysis of Human Functional Transcription Factor Binding Reveals a Strong Bias towards the Transcription Start Site [PDF]

PLoS ONE Issue 8, e807 Aug 2007, 2008
We introduce a novel method to screen the promoters of a set of genes with shared biological function, against a precompiled library of motifs, and find those motifs which are statistically over-represented in the gene set. The gene sets were obtained from the functional Gene Ontology (GO) classification; for each set and motif we optimized the ...
A Ambesi-Impiombato, A Blais, A Eto, A Subramanian, AE Kel, AG Clark, AL Lam, AM McGuire, Anat Reiner, Assif Yitzhaky, B Ren, C Kimura-Yoshida, C Plessy, C Yang, CT Harbison, D Pfeifer, D Wang, DB Allison, E Emberly, E Segal, Eytan Domany, FP Roth, GC Pipes, GC Yuan, GQ Yao, GZ Hertz, H Li, H Lodish, J Zheng, JD Hughes, JL DeRisi, JQ Ling, K Frech, K Quandt, KD MacIsaac, L Amir-Zilberstein, L Elnitski, L Marino-Ramirez, L McCue, M Ashburner, M Kellis, M Milyavsky, MA Nobrega, Mark Koudritsky, MC Frith, ML Howard, ML Whitfield, N Rajewsky, Or Zuk, P Carninci, P Carninci, P Cliften, PM Haverty, PR Buckland, R Elkon, R Liu, R Sharan, Ran Brosh, S Aerts, S Rashi-Elkeles, S Tavazoie, SJ Cooper, SJ Ho Sui, Sui Huang, U Gerland, Varda Rotter, WW Wasserman, X Xie, Y Barash, Y Benjamini, Y Benjamini, Y Tabach, Yossi Buganim, Yuval Tabach, Z Wang   +74 more
arxiv   +7 more sources

Construction of cDNA library of Dalbergia odorifera induced by low temperature stress and screening of low temperature tolerant genes. [PDF]

PLoS ONE
To systematically analyze the gene function of Dalbergia odorifera, the seedlings of D. odorifera were treated with low-temperature stress for 6 h. Total RNA was extracted from a mixture of seedling roots, stems, and leaves, and a low-temperature-induced
Shaocui Li, Xia An, Fayong Li, Yining Chen, Xiaowen Li   +4 more
doaj   +2 more sources

Library Generation by Gene Shuffling [PDF]

Current Protocols in Molecular Biology, 2014
AbstractThis unit describes the process of gene shuffling, also known as sexual PCR. Gene shuffling is a facile method for the generation of sequence libraries containing the information from a family of related genes. Essentially, related genes are fragmented by DNase I digestion and reassembled by primer‐less PCR.
Adam J. Meyer, Jared W. Ellefson, Andrew D. Ellington   +2 more
openaire   +3 more sources