A high-throughput method for Illumina RNA-Seq library preparation [PDF]
Frontiers in Plant Science, 2012 With the introduction of cost effective, rapid and superior quality next generation sequencing (NGS) techniques, gene expression analysis has become viable for labs conducting small projects as well as large-scale gene expression analysis experiments ...
Ravi eKumar +8 more
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The COVID-19 Drug and Gene Set Library [PDF]
Patterns, 2020 Summary: In a short period, many research publications that report sets of experimentally validated drugs as potential COVID-19 therapies have emerged.
Maxim V. Kuleshov +13 more
doaj +4 more sources
Variation in guide RNA library representation results in gene effect score bias in genome-wide CRISPR screens [PDF]
BMC GenomicsGenome wide CRISPR-based perturbation screens are powerful discovery tools enabling the identification of novel gene dependencies through either gain or loss of function. While genome wide guide RNA (gRNA) libraries have advantages when using enAsCas12a,
Paul Metz +5 more
doaj +2 more sources
A gene-specific T2A-GAL4 library for Drosophila
eLife, 2018 We generated a library of ~1000 Drosophila stocks in which we inserted a construct in the intron of genes allowing expression of GAL4 under control of endogenous promoters while arresting transcription with a polyadenylation signal 3’ of the GAL4.
Pei-Tseng Lee +21 more
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B. subtilis MutS2 splits stalled ribosomes into subunits without mRNA cleavage
The EMBO Journal, 2023 Stalled ribosomes are rescued by pathways that recycle the ribosome and target the nascent polypeptide for degradation. In E. coli, these pathways are triggered by ribosome collisions through the recruitment of SmrB, a nuclease that cleaves the mRNA.
Esther N Park +10 more
doaj +1 more source
Library Generation by Gene Shuffling [PDF]
Current Protocols in Molecular Biology, 2014 AbstractThis unit describes the process of gene shuffling, also known as sexual PCR. Gene shuffling is a facile method for the generation of sequence libraries containing the information from a family of related genes. Essentially, related genes are fragmented by DNase I digestion and reassembled by primer‐less PCR.
Adam J, Meyer +2 more
openaire +2 more sources
A Robust and Versatile Method of Combinatorial Chemical Synthesis of Gene Libraries via Hierarchical Assembly of Partially Randomized Modules. [PDF]
PLoS ONE, 2015 A major challenge in gene library generation is to guarantee a large functional size and diversity that significantly increases the chances of selecting different functional protein variants.
Blagovesta Popova +4 more
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RNAi as a Tool to Study Virulence in the Pathogenic Yeast Candida glabrata
Frontiers in Microbiology, 2019 The yeast Candida glabrata is a major opportunistic pathogen causing mucosal and systemic infections in humans. Systemic infections caused by this yeast have high mortality rates and are difficult to treat due to this yeast’s intrinsic and frequently ...
Olena P. Ishchuk +19 more
doaj +1 more source
Solid Phase Assembly of Fully Protected Trinucleotide Building Blocks for Codon-Based Gene Synthesis
Applied Sciences, 2019 The use of pre-formed trinucleotides, representing codons of the 20 canonical amino acids, for oligonucleotide-directed mutagenesis offers the advantage of controlled randomization and generation of “smart libraries”. We here present a method
Ruth Suchsland +3 more
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Cloning and expression of porcine β1,4 N-acetylgalactosaminyl transferase encoding a new xenoreactive antigen [PDF]
, 2014 Xenograft rejection of pigs organs with an engineered mutation in the GGTA-1 gene (GTKO) remains a predominantly antibody mediated process which is directed to a variety of non-Gal protein and carbohydrate antigens.
Byrne, GW +4 more
core +2 more sources