Results 11 to 20 of about 1,268,532 (356)
The COVID-19 Gene and Drug Set Library [PDF]
SSRN Electronic Journal, 2020 Abstract The coronavirus (CoV) severe acute respiratory syndrome (SARS)-CoV-2 (COVID-19) pandemic has received rapid response by the research community to offer suggestions for repurposing of approved drugs as well as to improve our understanding of the COVID-19 viral life cycle molecular mechanisms.
Maxim V. Kuleshov +9 more
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A Robust and Versatile Method of Combinatorial Chemical Synthesis of Gene Libraries via Hierarchical Assembly of Partially Randomized Modules. [PDF]
PLoS ONE, 2015 A major challenge in gene library generation is to guarantee a large functional size and diversity that significantly increases the chances of selecting different functional protein variants.
Blagovesta Popova +4 more
doaj +1 more source
GOATOOLS: A Python library for Gene Ontology analyses [PDF]
Scientific Reports, 2018 AbstractThe biological interpretation of gene lists with interesting shared properties, such as up- or down-regulation in a particular experiment, is typically accomplished using gene ontology enrichment analysis tools. Given a list of genes, a gene ontology (GO) enrichment analysis may return hundreds of statistically significant GO results in a “flat”
D. V. Klopfenstein +13 more
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CRISPR/Cas9 high-throughput screening in cancer research [PDF]
E3S Web of Conferences, 2020 In recent years, CRISPR/Cas9 technology has developed rapidly. With its accurate, fast, and simple editing functions that can achieve gene activation, interference, knockout, and knock-in, it has become a powerful genetic screening tool that is widely ...
Liu Zhuoxin
doaj +1 more source
Adventures in the enormous: a 1.8 million clone BAC library for the 21.7 Gb genome of loblolly pine. [PDF]
PLoS ONE, 2011 Loblolly pine (LP; Pinus taeda L.) is the most economically important tree in the U.S. and a cornerstone species in southeastern forests. However, genomics research on LP and other conifers has lagged behind studies on flowering plants due, in part, to ...
Zenaida V Magbanua +8 more
doaj +1 more source
RNAi as a Tool to Study Virulence in the Pathogenic Yeast Candida glabrata
Frontiers in Microbiology, 2019 The yeast Candida glabrata is a major opportunistic pathogen causing mucosal and systemic infections in humans. Systemic infections caused by this yeast have high mortality rates and are difficult to treat due to this yeast’s intrinsic and frequently ...
Olena P. Ishchuk +19 more
doaj +1 more source
Solid Phase Assembly of Fully Protected Trinucleotide Building Blocks for Codon-Based Gene Synthesis
Applied Sciences, 2019 The use of pre-formed trinucleotides, representing codons of the 20 canonical amino acids, for oligonucleotide-directed mutagenesis offers the advantage of controlled randomization and generation of “smart libraries”. We here present a method
Ruth Suchsland +3 more
doaj +1 more source
RnaseIII and T4 Polynucleotide Kinase Sequence Biases and Solutions During RNA-Seq Library Construction [PDF]
, 2013 Background: RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement.
Changhoon Lee +5 more
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Analysis of shuffled gene libraries
Journal of Molecular Biology, 2002 In vitro recombination of homologous genes (family shuffling) has been proposed as an effective search strategy for laboratory evolution of genes and proteins. Few data are available, however, on the composition of shuffled gene libraries, from which one could assess the efficiency of recombination and optimize protocols.
Joern, John M +2 more
openaire +4 more sources
Nature Materials, 2011 By combining gene cloning and amplification techniques, a new one-pot, parallel synthesis method for the generation of long, repetitive genes is realized. The method promises to open up the discovery of protein polymer biomaterials.
Sheng Ding +2 more
openaire +2 more sources