A gene-specific T2A-GAL4 library for Drosophila
eLife, 2018 We generated a library of ~1000 Drosophila stocks in which we inserted a construct in the intron of genes allowing expression of GAL4 under control of endogenous promoters while arresting transcription with a polyadenylation signal 3’ of the GAL4.
Pei-Tseng Lee +21 more
doaj +4 more sources
Library Generation by Gene Shuffling [PDF]
Current Protocols in Molecular Biology, 2014 AbstractThis unit describes the process of gene shuffling, also known as sexual PCR. Gene shuffling is a facile method for the generation of sequence libraries containing the information from a family of related genes. Essentially, related genes are fragmented by DNase I digestion and reassembled by primer‐less PCR.
Adam J, Meyer +2 more
openaire +2 more sources
Organization and sequence of the gene encoding the human acrosin-trypsin inhibitor (HUSI-II) [PDF]
, 1993 A complete cDNA encoding the acrosin-trypsin inhibitor, HUSI-II, was used as a probe to isolate genomic clones from a human placenta library. Three clones which cover the entire HUSI-II gene were isolated and characterized.
Abelson +28 more
core +1 more source
Cloning and characterisation of the S.pombe rad15 gene, a homologue to the S.cerevisiae RAD3 and human ERCC2 genes [PDF]
, 1992 The RAD3 gene of Saccharomyces cerevisiae encodes an ATP-dependent 5' - 3' DNA helicase, which is involved in excision repair of ultraviolet radiation damage.
Alan R. Lehmann +6 more
core +3 more sources
Short-hairpin RNA library: identification of therapeutic partners for gefitinib-resistant non-small cell lung cancer. [PDF]
, 2014 Somatic mutations of the epidermal growth factor receptor often cause resistance to therapy with tyrosine kinase inhibitor in non-small cell lung cancer (NSCLC). In this study, we aimed to identify partner drugs and pathways that can induce cell death in
Koeffler, H Phillip +5 more
core +2 more sources
RnaseIII and T4 Polynucleotide Kinase Sequence Biases and Solutions During RNA-Seq Library Construction [PDF]
, 2013 Background: RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement.
Changhoon Lee +5 more
core +3 more sources
Analysis of shuffled gene libraries
Journal of Molecular Biology, 2002 In vitro recombination of homologous genes (family shuffling) has been proposed as an effective search strategy for laboratory evolution of genes and proteins. Few data are available, however, on the composition of shuffled gene libraries, from which one could assess the efficiency of recombination and optimize protocols.
Joern, John M +2 more
openaire +3 more sources
Mutant non-coding RNA resource in mouse embryonic stem cells
Disease Models & Mechanisms, 2021 Gene trapping is a high-throughput approach that has been used to introduce insertional mutations into the genome of mouse embryonic stem (ES) cells. It is performed with generic gene trap vectors that simultaneously mutate and report the expression of ...
Jens Hansen +2 more
doaj +1 more source
Preparation of trinucleotide phosphoramidites as synthons for the synthesis of gene libraries
Beilstein Journal of Organic Chemistry, 2018 The preparation of protein libraries is a key issue in protein engineering and biotechnology. Such libraries can be prepared by a variety of methods, starting from the respective gene library.
Ruth Suchsland +2 more
doaj +1 more source
htsint: a Python library for sequencing pipelines that combines data through gene set generation [PDF]
, 2015 Background: Sequencing technologies provide a wealth of details in terms of genes, expression, splice variants, polymorphisms, and other features. A standard for sequencing analysis pipelines is to put genomic or transcriptomic features into a context of
Bonneaud, Camille +2 more
core +3 more sources