Engineering cell factories for producing biofuels and pharmaceuticals has spurred great interests to develop rapid and efficient synthetic biology tools customized for modular pathway engineering.
Erqing Jin +5 more
doaj +1 more source
Modifications at the C-Terminus To Improve Pyrrole−Imidazole Polyamide Activity in Cell Culture [PDF]
Pyrrole−imidazole (Py-Im) hairpin polyamides are a class of small molecule DNA minor groove binding compounds that have been shown to modulate endogenous gene expression in cell culture.
Dervan, Peter B., Jacobs, Claire S.
core +2 more sources
Screening technology of cyclic peptide library based on gene encoding
As a powerful tool for the discovery of peptide compounds, high-throughput library screening technology can also perform multitarget screening with high chemical diversity as a condition, which accelerates the process of exploring high-affinity peptide ...
Yangqiang Chen +5 more
doaj +1 more source
Isolation of pigment cell specific genes in the sea urchin embryo by differential macroarray screening [PDF]
New secondary mesenchyme specific genes, expressed exclusively in pigment cells, were isolated from sea urchin embryos using a differential screening of a macroarray cDNA library. The comparison was performed between mRNA populations of embryos having an
Calestani, Cristina +2 more
core +1 more source
Screening of cis-acting elements for maintaining pluripotency of human embryonic stem cells [PDF]
Objective To establish an inducible CRISPR library screening method for human embryonic stem cells (hESCs) to screen the cis-acting elements involed in the maintainance the pluripotency of hESCs.
SUN Meng-yao, LIU Si-qi, ZHOU Fan-qi, MA Yan-ni, YU Jia
doaj
Development of a selection system for the detection of L-ribose isomerase expressing mutants of Escherichia coli [PDF]
L-Arabinose isomerase (E.C. 5.3.1.14) catalyzes the reversible isomerization between L-arabinose and L-ribulose and is highly selective towards L-arabinose.
Beauprez, Joeri +7 more
core +1 more source
ERgene: Python library for screening endogenous reference genes [PDF]
AbstractIn gene expression analysis, sample differences and experimental operation differences are common, but sometimes, these differences will cause serious errors to the results or even make the results meaningless. Finding suitable internal reference genes efficiently to eliminate errors is a challenge.
Zeng, Zehua +3 more
openaire +2 more sources
Cloning of a Transglutaminase Gene from an Indonesian Streptomyces Strain
Microbial Transglutaminase (MTGase, EC 2.3.2.13) is an enzyme that catalyzes the transfer of acyl group. Many microbial strains that produce MTGase belong to Streptomyces members. This research was aimed at cloning of a MTGase gene.
Seprianto Chaniago
doaj +1 more source
Microbial population changes during bioremediation of nitroaromatic - and nitramine-contaminated lagoon [PDF]
Nitration reactions of aromatic compounds are commonly involved in military industrial processes. Military industries treated their process effluents using lagoon systems for many years.
Goñi, Marisol +5 more
core +1 more source
Generation and characterization of expressed sequence tags in young roots of tea (Camellia assamica)
Assam tea (Camellia assamica) is perennial crop susceptible to moisture stress. We used its tender roots to construct a cDNA library for the identification, functional annotation, and analysis of transcripts. A total of 811 full-length expressed sequence
A. Das, M. Mukhopadhyay, T. K. Mondal
doaj +1 more source

