Results 41 to 50 of about 1,268,532 (356)
Exploring nervous system transcriptomes during embryogenesis and metamorphosis in Xenopus tropicalis using EST analysis [PDF]
BMC Genomics 8 (2007) 118, 2007 Xenopus tropicalis is an anuran amphibian species used as model in vertebrate comparative genomics. It provides the same advantages as Xenopus laevis but is diploid and has a smaller genome of 1.7 Gbp. Therefore X. tropicalis is more amenable to systematic transcriptome surveys.
arxiv +1 more source
Isolation of pigment cell specific genes in the sea urchin embryo by differential macroarray screening [PDF]
, 2003 New secondary mesenchyme specific genes, expressed exclusively in pigment cells, were isolated from sea urchin embryos using a differential screening of a macroarray cDNA library. The comparison was performed between mRNA populations of embryos having an
Calestani, Cristina +2 more
core +1 more source
Identification of functional genes by libraries of ribozymes and siRNAs [PDF]
Nucleic Acids Symposium Series, 2003 Libraries were made of hammerhead ribozymes with randomized binding arms and/or of U6 and tRNA-driven siRNAs. Then, the libraries were introduced into cells either by transfection or by viral vectors. This procedure made it possible to readily identify the relevant genes associated with phenotype in the apoptosis, cancer metastasis, and/or cell ...
Hiroaki Kawasaki +3 more
openaire +3 more sources
Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife, 2020 Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-throughput sequencing applications. Here, we report that the Tn5 transposase is also capable of direct tagmentation of RNA/DNA hybrids in vitro.
Bo Lu +6 more
doaj +1 more source
Generating site saturation mutagenesis libraries and transferring them to broad host range plasmids using type IIS restriction enzymes [PDF]
arXiv, 2023 Protein engineering is an established method for tailoring enzymatic reactivity. A commonly used method is directed evolution, where the mutagenesis and natural selection process is mimicked and accelerated in the laboratory. Here, we describe a reliable method for generating saturation mutagenesis libraries by golden gate cloning in a broad host range
arxiv
PLoS ONE, 2017 Bovine viral diarrhea-mucosal disease (BVD-MD) is caused by bovine viral diarrhea virus (BVDV), and results in abortion, stillbirth, and fetal malformation in cows. Here, we constructed the phage display vector pCANTAB 5E-VHH and then transformed it into
Tiansen Li +8 more
doaj +1 more source
An improved shotgun antisense method for mutagenesis and gene identification
BioTechniques, 2020 Shotgun expression of antisense cDNA, where each transformed cell expresses a different antisense cDNA, has been used for mutagenesis and gene identification in Dictyostelium discoideum. However, the method has two limitations.
Yu Tang, Richard H Gomer
doaj +1 more source
Inference of nonlinear causal effects with GWAS summary data [PDF]
arXiv, 2022 Large-scale genome-wide association studies (GWAS) have offered an exciting opportunity to discover putative causal genes or risk factors associated with diseases by using SNPs as instrumental variables (IVs). However, conventional approaches assume linear causal relations partly for simplicity and partly for the availability of GWAS summary data.
arxiv
Identification of novel small molecule inhibitors of ETS transcription factors
FEBS Letters, EarlyView.ETS transcription factors play an essential role in tumourigenesis and are indispensable for sprouting angiogenesis, a hallmark of cancer, which fuels tumour expansion and dissemination. Thus, targeting ETS transcription factor function could represent an effective, multifaceted strategy to block tumour growth. The evolutionarily conserved E‐Twenty‐Six
Shaima Abdalla +9 more
wiley +1 more source
Frontiers in Microbiology, 2012 Sphagnum mosses represent a main component in ombrotrophic wetlands. They harbor a specific and diverse microbial community with essential functions for the host. To understand extend and degree of host specificity, Sphagnum fallax and S.
Anastasia eBragina +6 more
doaj +1 more source