Results 41 to 50 of about 1,350,251 (258)

Identification of host factors for livestock and poultry viruses: genome-wide screening technology based on the CRISPR system

Frontiers in Microbiology
Genome-wide CRISPR library screening technology is a gene function research tool developed based on the CRISPR/Cas9 gene-editing system. The clustered regularly interspaced short palindromic repeats/CRISPR-associated genes (CRISPR/Cas) system, considered
Shijie Hu, Shijie Hu, Mailin Gan, Mailin Gan, Ziang Wei, Ziang Wei, Pan Shang, Pan Shang, Lei Song, Lei Song, Jinkang Feng, Jinkang Feng, Lei Chen, Lei Chen, Lili Niu, Lili Niu, Yan Wang, Yan Wang, Yan Wang, Shunhua Zhang, Shunhua Zhang, Linyuan Shen, Linyuan Shen, Linyuan Shen, Li Zhu, Li Zhu, Li Zhu, Ye Zhao, Ye Zhao, Ye Zhao   +29 more
doaj   +1 more source

Construction of a combinatorial library of chimeric tumor-specific promoters

BioTechniques, 2017
Gene therapy is a fast-developing field of molecular medicine. New, effective, and cancer-specific promoters are in high demand by researchers seeking to treat cancer through expression of therapeutic genes.
Kirill N. Kashkin, Igor P. Chernov, Dmitry A. Didych, Eugene D. Sverdlov   +3 more
doaj   +1 more source

Wide-Scale Analysis of Human Functional Transcription Factor Binding Reveals a Strong Bias towards the Transcription Start Site [PDF]

, 2007
We introduce a novel method to screen the promoters of a set of genes with shared biological function, against a precompiled library of motifs, and find those motifs which are statistically over-represented in the gene set.
A Ambesi-Impiombato, A Blais, A Eto, A Subramanian, AE Kel, AG Clark, AL Lam, AM McGuire, Anat Reiner, Assif Yitzhaky, B Ren, C Kimura-Yoshida, C Plessy, C Yang, CT Harbison, D Pfeifer, D Wang, DB Allison, E Emberly, E Segal, Eytan Domany, FP Roth, GC Pipes, GC Yuan, GQ Yao, GZ Hertz, H Li, H Lodish, J Zheng, JD Hughes, JL DeRisi, JQ Ling, K Frech, K Quandt, KD MacIsaac, L Amir-Zilberstein, L Elnitski, L Marino-Ramirez, L McCue, M Ashburner, M Kellis, M Milyavsky, MA Nobrega, Mark Koudritsky, MC Frith, ML Howard, ML Whitfield, N Rajewsky, Or Zuk, P Carninci, P Carninci, P Cliften, PM Haverty, PR Buckland, R Elkon, R Liu, R Sharan, Ran Brosh, S Aerts, S Rashi-Elkeles, S Tavazoie, SJ Cooper, SJ Ho Sui, Sui Huang, U Gerland, Varda Rotter, WW Wasserman, X Xie, Y Barash, Y Benjamini, Y Benjamini, Y Tabach, Yossi Buganim, Yuval Tabach, Z Wang   +74 more
core   +5 more sources

Massive integration of large gene libraries in the chromosome of Escherichia coli

Microbial Biotechnology
Large gene libraries are frequently created in Escherichia coli plasmids, which can induce cell toxicity and expression instability due to the high gene dosage.
Lidia Cerdán, Beatriz Álvarez, Luis Ángel Fernández   +2 more
doaj   +1 more source

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens

G3: Genes, Genomes, Genetics, 2017
The adaptation of CRISPR/SpCas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs) targeting human protein-coding genes and encoded in viral vectors have been used to ...
Traver Hart, Amy Hin Yan Tong, Katie Chan, Jolanda Van Leeuwen, Ashwin Seetharaman, Michael Aregger, Megha Chandrashekhar, Nicole Hustedt, Sahil Seth, Avery Noonan, Andrea Habsid, Olga Sizova, Lyudmila Nedyalkova, Ryan Climie, Leanne Tworzyanski, Keith Lawson, Maria Augusta Sartori, Sabriyeh Alibeh, David Tieu, Sanna Masud, Patricia Mero, Alexander Weiss, Kevin R. Brown, Matej Usaj, Maximilian Billmann, Mahfuzur Rahman, Michael Constanzo, Chad L. Myers, Brenda J. Andrews, Charles Boone, Daniel Durocher, Jason Moffat   +31 more
doaj   +1 more source

Establishment of a CRISPR-Based Lentiviral Activation Library for Transcription Factor Screening in Porcine Cells

Animals
Transcription factors play important roles in the growth and development of various tissues in pigs, such as muscle, fat, and bone. A transcription-factor-scale activation library based on the clustered, regularly interspaced, short palindromic repeat ...
Yingjuan Liang, Xiaoxia Yao, Jingxin Han, Jinpeng Wang, Xiao Zhang, Donglin Zhao, Chaoqian Jiang, Lishuang Geng, Shihao Lv, Zhonghua Liu, Yanshuang Mu   +10 more
doaj   +1 more source

The Impact of cDNA Normalization on Long-Read Sequencing of a Complex Transcriptome

Frontiers in Genetics, 2019
Normalization of cDNA is widely used to improve the coverage of rare transcripts in analysis of transcriptomes employing next-generation sequencing. Recently, long-read technology has been emerging as a powerful tool for sequencing and construction of ...
Nam V. Hoang, Agnelo Furtado, Virginie Perlo, Frederik C. Botha, Frederik C. Botha, Robert J. Henry   +5 more
doaj   +1 more source

cDNA and Gene Analyses Imply a Novel Structure for a Rat Carcinoembryonic Antigen-related Protein [PDF]

, 1990
The gene encoding the human tumor marker carcinoembryonic antigen (CEA) belongs to a gene family which can be subdivided into the CEA and the pregnancy-specific glycoprotein subgroups.
Lucas, Kurt, Rebstock, Sabine, Thompson, John A., Zimmermann, Wolfgang   +3 more
core  

The National Laboratory Gene Library Project [PDF]

, 1989
The two National Laboratories at Livermore and Los Alamos have played a prominent role in the development and application of flow cytometry and sorting to chromosome classification and purification. Both laboratories began to receive numerous requests for specific human chromosomal types purified by flow sorting for gene library construction, but these
M.A. VAN DILLA, N.A. ALLEN, A.V. CARRANO, M. CHRISTENSEN, P. DE JONG, P.N. DEAN, J.C. FUSCOE, J.W. GRAY, C.R. LOZES, J.S. McNINCH, M.L. MENDELSOHN, J. MULLIKIN, L. PEDERSON, J. PERLMAN, D.C. PETERS, A.J. SILVA, B.J. TRASK, G.J. VAN DEN ENGH, L.L. DEAVEN, K.L. ALBRIGHT, M.F. BARTHOLDI, N.C. BROWN, E.W. CAMPBELL, L.M. CLARK, L.S. CRAM, J.J. FAWCETT, C.E. HILDEBRAND, P.J. JACKSON, J.H. JETT, S. KOLLA, J.L. LONGMIRE, M.L. LUEDEMANN, J. MEYNE, L.J. MEINCKE, R.K. MOYZIS, A.C. MUNK   +35 more
openaire   +1 more source

Construction of Gene Targeting Vectors from λKOS Genomic Libraries

BioTechniques, 1999
We describe a highly redundant murine genomic library in a new λ phage, λ knockout shuttle (λKOS) that facilitates the very rapid construction of replacement-type gene targeting vectors.
Sigrid Wattler, Mike Kelly, Michael Nehls   +2 more
doaj   +1 more source