Results 51 to 60 of about 1,329,302 (305)

Plasma extrachromosomal circular DNA as a biomarker in EGFR‐targeted therapy of non‐small cell lung cancer

Molecular Oncology, EarlyView.
Detection of extrachromosomal circular DNA (eccDNA) in plasma samples from EGFR‐mutated non‐small cell lung cancer patients. Plasma was collected before and during treatment with the EGFR‐tyrosine kinase inhibitor osimertinib. Plasma eccDNA was detected in all cancer samples, and the presence of the EGFR gene on eccDNA serves as a potential biomarker ...
Simone Stensgaard, Sarmad Mehmood, Eva Boysen Fynboe Ebert, Peter Meldgaard, Anindya Dutta, Boe S. Sorensen   +5 more
wiley   +1 more source

Identification of Sequences Encoding Symbiodinium minutum Mitochondrial Proteins. [PDF]

, 2016
The dinoflagellates are an extremely diverse group of algae closely related to the Apicomplexa and the ciliates. Much work has previously been undertaken to determine the presence of various biochemical pathways within dinoflagellate mitochondria ...
Butterfield, Erin R., Howe, Christopher J., Nisbet, R. Ellen R.   +2 more
core   +4 more sources

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

The National Laboratory Gene Library Project [PDF]

, 1989
The two National Laboratories at Livermore and Los Alamos have played a prominent role in the development and application of flow cytometry and sorting to chromosome classification and purification. Both laboratories began to receive numerous requests for specific human chromosomal types purified by flow sorting for gene library construction, but these
M.A. VAN DILLA, N.A. ALLEN, A.V. CARRANO, M. CHRISTENSEN, P. DE JONG, P.N. DEAN, J.C. FUSCOE, J.W. GRAY, C.R. LOZES, J.S. McNINCH, M.L. MENDELSOHN, J. MULLIKIN, L. PEDERSON, J. PERLMAN, D.C. PETERS, A.J. SILVA, B.J. TRASK, G.J. VAN DEN ENGH, L.L. DEAVEN, K.L. ALBRIGHT, M.F. BARTHOLDI, N.C. BROWN, E.W. CAMPBELL, L.M. CLARK, L.S. CRAM, J.J. FAWCETT, C.E. HILDEBRAND, P.J. JACKSON, J.H. JETT, S. KOLLA, J.L. LONGMIRE, M.L. LUEDEMANN, J. MEYNE, L.J. MEINCKE, R.K. MOYZIS, A.C. MUNK   +35 more
openaire   +1 more source

A binary vector for transferring genomic libraries to plants [PDF]

, 1986
The transformation of mutant plants with a complete recombinant library derived from wild-type DNA followed by assay of transformed plants for complementation of the mutant phenotype is a promising method for the isolation of plant genes.
Simoens, Chris, Alliotte, Thierry, Mendel, R, Müller, A, Schiemann, J, Van Lijsebettens, Maria, Schell, Jeff, Van Montagu, Marc, Inzé, Dirk   +8 more
core   +1 more source

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source

Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens

G3: Genes, Genomes, Genetics, 2017
The adaptation of CRISPR/SpCas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs) targeting human protein-coding genes and encoded in viral vectors have been used to ...
Traver Hart, Amy Hin Yan Tong, Katie Chan, Jolanda Van Leeuwen, Ashwin Seetharaman, Michael Aregger, Megha Chandrashekhar, Nicole Hustedt, Sahil Seth, Avery Noonan, Andrea Habsid, Olga Sizova, Lyudmila Nedyalkova, Ryan Climie, Leanne Tworzyanski, Keith Lawson, Maria Augusta Sartori, Sabriyeh Alibeh, David Tieu, Sanna Masud, Patricia Mero, Alexander Weiss, Kevin R. Brown, Matej Usaj, Maximilian Billmann, Mahfuzur Rahman, Michael Constanzo, Chad L. Myers, Brenda J. Andrews, Charles Boone, Daniel Durocher, Jason Moffat   +31 more
doaj   +1 more source

Creating E.coli Library of Diagnostic Gene for Influenza B Virus Using Molecular Cloning Technique [PDF]

, 2014
Influenza viruses cause seasonal epidemics associated with high morbidity, especially in immunocompromised and extreme age group individuals. Many strategies for combating these viruses are in practice; however, developing and maintaining sensitive ...
Chinbat, Oyunsuvd
core  

Generation and analysis of expressed sequence tags from Botrytis cinerea [PDF]

, 2006
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200018&lng=es&nrm=isoBotrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in ...
Holmes, David, Shmaryahu, Amir, Silva, Evelyn, Valdez, Jorge, Valenzuela, Pablo   +4 more
core   +2 more sources

Monitoring of circulating tumor DNA allows early detection of disease relapse in patients with operable breast cancer

Molecular Oncology, EarlyView.
Monitoring circulating tumor DNA (ctDNA) in patients with operable breast cancer can reveal disease relapse earlier than radiology in a subset of patients. The failure to detect ctDNA in some patients with recurrent disease suggests that ctDNA could serve as a supplement to other monitoring approaches.
Kristin Løge Aanestad, Marie Austdal, Oddmund Nordgård, Gunnar Mellgren, Satu Oltedal, Marie L. Austbø, Ylva H. Vignes, Thomas Helland, Kristin Jonsdottir, Tone H. Lende, Emilius A. M. Janssen, Bjørnar Gilje, Kjersti Tjensvoll, PBCB study group, Gunnar Mellgren, Tone Hoel Lende, Anette Heie, Kristin Viste, Anita Røyneberg Alvheim, Emiel AM Janssen, Kristin Jonsdottir, Ann Cathrine Kroksveen, Thomas Helland, Einar Gudlaugsson, Oddmund Nordgård, Satu Oltedal, Kristin Løge Aanestad, Jan Terje Kvaløy, Kirsten Lode, Kari Britt Hagen, Marie Austdal, Ylva H. Vignes, Siri Tungland Sola, Nina Egeland Amundsen, Finn Magnus Eliassen, Emeritus Ernst A Lien   +35 more
wiley   +1 more source