Results 71 to 80 of about 1,268,532 (356)
RNA modulators of complex phenotypes in mammalian cells. [PDF]
PLoS ONE, 2009 RNA-mediated gene silencing, in the form of RNA interference (RNAi) or microRNAs (miRNAs) has provided novel tools for gene discovery and validation in mammalian cells.
Angela Lai +5 more
doaj +1 more source
Selection of chromosomal DNA libraries using a multiplex CRISPR system. [PDF]
, 2014 The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that ...
Arkin, Adam P +10 more
core +2 more sources
Molecular Oncology, EarlyView.The authors analyzed the spatial distributions of gene and metabolite profiles in cervical cancer through spatial transcriptomic and spatially resolved metabolomic techniques. Pivotal genes and metabolites within these cases were then identified and validated.
Lixiu Xu +3 more
wiley +1 more source
Conversion of cDNA differential display results (DDRT-PCR) into quantitative transcription profiles [PDF]
BMC Genomics 6/51 (2005) 1-12, 2014 Background: Gene expression studies on non-model organisms require open-end strategies for transcription profiling. Gel-based analysis of cDNA fragments allows to detect alterations in gene expression for genes which have neither been sequenced yet nor are available in cDNA libraries. Commonly used protocols are cDNA Differential Display (DDRT-PCR) and
arxiv +1 more source
SpZ12-1, a negative regulator required for spatial control of the territory-specific CyIIIa gene in the sea urchin embryo [PDF]
, 1995 The CyIIIa cytoskeletal actin gene of the sea urchin Strongylocentrotus purpuratus is activated in late cleavage and expressed exclusively in the aboral ectoderm territory of the embryo.
Britten, Roy J. +3 more
core
Molecular Oncology, EarlyView.The authors applied joint/mixed models that predict mortality of trifluridine/tipiracil‐treated metastatic colorectal cancer patients based on circulating tumor DNA (ctDNA) trajectories. Patients at high risk of death could be spared aggressive therapy with the prospect of a higher quality of life in their remaining lifetime, whereas patients with a ...
Matthias Unseld +7 more
wiley +1 more source
Construction of Gene Targeting Vectors from λKOS Genomic Libraries
BioTechniques, 1999 We describe a highly redundant murine genomic library in a new λ phage, λ knockout shuttle (λKOS) that facilitates the very rapid construction of replacement-type gene targeting vectors.
Sigrid Wattler +2 more
doaj +1 more source
Representative cDNA Libraries and Their Utility in Gene Expression Profiling [PDF]
BioTechniques, 1999 An increasing interest in gene expression profiles in human diseases has led to the use of microdissected tumors and biopsies in gene discovery approaches. Since many of these clinical samples yield extremely small amounts of RNA, reproducible methods are needed to amplify this RNA while maintaining the original message profile.
Wilson O. Endege +4 more
openaire +3 more sources
Crosstalk between gut microbiota and tumor: tumors could cause gut dysbiosis and metabolic imbalance
Molecular Oncology, EarlyView.In this research, we analyzed the relationship between gut microbiota and tumor. We discovered that both subcutaneous and metastatic tumors would alter the composition and metabolic function of gut microbiota. Meanwhile, fecal microbiota transplantation also indicated the anti‐tumor role of the gut microbiota, revealing the crosstalk between tumor and ...
Siyuan Zhang +8 more
wiley +1 more source
Estimating the Number of Essential Genes in Random Transposon Mutagenesis Libraries [PDF]
arXiv, 2006 Biologists use random transposon mutagenesis to construct knockout libraries for bacteria. Random mutagenesis offers cost and efficiency benefits over the standard site directed mutagenesis, but one can no longer ensure that all the nonessential genes will appear in the library.
arxiv