Results 101 to 110 of about 7,098,593 (382)

Intramyocardial gene silencing by interfering RNA [PDF]

, 2017
RNAi is a widely used methodology for gene silencing. The action mechanism of siRNA molecules has been well studiedin recent years, and the technique has been optimized in terms of safety and effectiveness. Cardiovascular diseases havea high incidence in
Brea, María Soledad, Morgan, Patricio Eduardo, Perez, Nestor Gustavo   +2 more
core   +1 more source

Loss of proton‐sensing GPR4 reduces tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
G protein‐coupled receptor 4 (GPR4) is a pH‐sensing receptor activated by acidic pH. GPR4 expression is increased in patients with inflammatory bowel disease who are at high risk of developing colorectal cancer. In mouse models, loss of GPR4 attenuated tumor progression. This correlated with increased IL2 and natural killer cell activity.
Leonie Perren, Moana Busch, Pedro A. Ruiz, Ermanno Malagola, Valeria Baumeler, Federica Foti, Adelina Gross, Tobias Grütter, Hendrik Edel, Cordelia Schuler, Kristina Handler, Glenn De Lange, Isabelle C. Arnold, Cheryl de Vallière, Klaus Seuwen, Martin Hausmann, Gerhard Rogler   +16 more
wiley   +1 more source

An enhanced CRISPR repressor for targeted mammalian gene regulation. [PDF]

, 2018
The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility.
Cecchi, Ryan J, Chan, Yingleong, Chavez, Alejandro, Church, George M, Church, George M, Collins, James J, Davidsohn, Noah, Ebrahimkhani, Mo R, Guo, Xiaoge, Kiani, Samira, Kuo, Chih-Chung, Lance-Byrne, Alissa, Lewis, Nathan E, Lim, Elaine T, Menn, David, Milanova, Denitsa, Pradhan, Swechchha, Sharma, Sumana, Tung, Angela, Tuttle, Marcelle, Yeo, Nan Cher   +20 more
core   +1 more source

Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots

Plant Methods, 2018
Aim We report the construction of a Virus-Induced Gene Silencing (VIGS) vector and an agroinoculation protocol for gene silencing in cassava (Manihot esculenta Crantz) leaves and roots.
Ezequiel Matias Lentz, Joel-Elias Kuon, Adrian Alder, Nathalie Mangel, Ima M. Zainuddin, Emily Jane McCallum, Ravi Bodampalli Anjanappa, Wilhelm Gruissem, Hervé Vanderschuren   +8 more
doaj   +1 more source

Lentivirus-delivered stable gene silencing by RNAi in primary cells.

RNA: A publication of the RNA Society, 2003
Genome-wide genetic approaches have proven useful for examining pathways of biological significance in model organisms such as Saccharomyces cerevisiae, Drosophila melanogastor, and Caenorhabditis elegans, but similar techniques have proven difficult to ...
S. Stewart, D. Dykxhoorn, D. Palliser, H. Mizuno, E. Yu, D. An, D. Sabatini, I. Chen, W. Hahn, P. Sharp, R. Weinberg, C. Novina   +11 more
semanticscholar   +1 more source

Systemic signalling in gene silencing [PDF]

Nature, 1997
Gene silencing in plants is a genetic control mechanism implicated in virus resistance1,2, genome maintenance3 and developmental control4. We describe here our recent discovery that there is a systemic signal that can mediate gene silencing. From the gene-specificity of the systemic silencing, we infer that the signal molecule is likely to be a nucleic
Olivier Voinnet, David C. Baulcombe
openaire   +3 more sources

TRPM8 levels determine tumor vulnerability to channel agonists

Molecular Oncology, EarlyView.
TRPM8 is a Ca2+ permissive channel. Regardless of the amount of its transcript, high levels of TRPM8 protein mark different tumors, including prostate, breast, colorectal, and lung carcinomas. Targeting TRPM8 with channel agonists stimulates inward calcium currents followed by emptying of cytosolic Ca2+ stores in cancer cells.
Alessandro Alaimo, Francesco Giuseppe Carbone, Kristi Buzo, Nicole Annesi, Sacha Genovesi, Annalisa Lorenzato, Karen Widmann, Michela Libergoli, Elisa Marmocchi, Giovanni Bertalot, Alberto Brolese, Mauro Giulio Papotti, Luca Molinaro, Orazio Caffo, Mattia Barbareschi, Alberto Bardelli, Alessandro Romanel, Sabrina Arena, Andrea Lunardi   +18 more
wiley   +1 more source

Targeting fungal genes by diced siRNAs: a rapid tool to decipher gene function in Aspergillus nidulans. [PDF]

PLoS ONE, 2013
BACKGROUND: Gene silencing triggered by chemically synthesized small interfering RNAs (siRNAs) has become a powerful tool for deciphering gene function in many eukaryotes.
Natarajaswamy Kalleda, Aruna Naorem, Rajam V Manchikatla   +2 more
doaj   +1 more source

SnapShot: Posttranscriptional Gene Silencing

Cell, 2007
Double-strand (ds)RNAs that are taken up exogenously, formed during viral replication, or generated from aberrant cellular RNAs are cut into 21–23 nucleotide (nt) siRNAs by Dicer in association with dsRNA-binding (dsRBD) protein. The siRNAs are then loaded into RISC, a complex containing Argonaute (Ago) protein.
Sigal Pressman, Richard W. Carthew, Yanxia Bei   +2 more
openaire   +2 more sources

The anticancer effect of the HDAC inhibitor belinostat is enhanced by inhibitors of Bcl‐xL or Mcl‐1 in ovarian cancer

Molecular Oncology, EarlyView.
The pan‐HDAC inhibitor belinostat increases the expression of the pro‐apoptotic proteins Bim, Puma, and Noxa and induces apoptosis in ovarian cancer cell lines and patient‐derived tumor organoids when used at high concentrations. Moreover, inhibiting the anti‐apoptotic proteins Bcl‐xL or Mcl‐1 sensitizes these preclinical models to the cytotoxic effect
Cécilia Thomine, Sterenn Guillemot, Louis‐Bastien Weiswald, Romane Florent, Edwige Abeilard, Florence Giffard, Emilie Brotin, Mélanie Briand, Enora Dolivet, Laurent Poulain, Marie Villedieu   +10 more
wiley   +1 more source