Results 261 to 270 of about 452,517 (308)
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Current Opinion in Genetics & Development, 2000
About two years ago, it was recognized that introduction of double-stranded RNA (dsRNA) had a potent effect on gene expression, in particular on mRNA stability. Since then, this process has been found to occur in many different organisms, and to bear a strong resemblance to a previously recognized process in plants, called cosuppression.
R H, Plasterk, R F, Ketting
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About two years ago, it was recognized that introduction of double-stranded RNA (dsRNA) had a potent effect on gene expression, in particular on mRNA stability. Since then, this process has been found to occur in many different organisms, and to bear a strong resemblance to a previously recognized process in plants, called cosuppression.
R H, Plasterk, R F, Ketting
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2016
Silencing of cardiac genes by RNA interference (RNAi) has developed into a powerful new method to treat cardiac diseases. Small interfering (si)RNAs are the inducers of RNAi, but cultured primary cardiomyocytes and heart are highly resistant to siRNA transfection.
Henry, Fechner +3 more
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Silencing of cardiac genes by RNA interference (RNAi) has developed into a powerful new method to treat cardiac diseases. Small interfering (si)RNAs are the inducers of RNAi, but cultured primary cardiomyocytes and heart are highly resistant to siRNA transfection.
Henry, Fechner +3 more
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2002
Gene silencing has evolved in a broad range of organisms probably as defense mechanisms against invasive nucleic acids. Two major strategies are utilized. Transcriptional gene silencing (TGS) acts to prevent RNA synthesis and posttranscriptional gene silencing (PTGS) acts to degrade existing RNA.
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Gene silencing has evolved in a broad range of organisms probably as defense mechanisms against invasive nucleic acids. Two major strategies are utilized. Transcriptional gene silencing (TGS) acts to prevent RNA synthesis and posttranscriptional gene silencing (PTGS) acts to degrade existing RNA.
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1995
“After such a survey one could readily come to the conclusion that the basis of the phenomenon is much too complex for profitable continuation of its investigation at this time”. [W.K. Baker (1968), in a review entitled “Position-effect variegation.”]
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“After such a survey one could readily come to the conclusion that the basis of the phenomenon is much too complex for profitable continuation of its investigation at this time”. [W.K. Baker (1968), in a review entitled “Position-effect variegation.”]
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Science, 2001
In diverse organisms, small RNAs derived from cleavage of double-stranded RNA can trigger epigenetic gene silencing in the cytoplasm and at the genome level. Small RNAs can guide posttranscriptional degradation of complementary messenger RNAs and, in plants, transcriptional gene silencing by methylation of homologous DNA sequences.
M, Matzke, A J, Matzke, J M, Kooter
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In diverse organisms, small RNAs derived from cleavage of double-stranded RNA can trigger epigenetic gene silencing in the cytoplasm and at the genome level. Small RNAs can guide posttranscriptional degradation of complementary messenger RNAs and, in plants, transcriptional gene silencing by methylation of homologous DNA sequences.
M, Matzke, A J, Matzke, J M, Kooter
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2022
An engineered transcriptional modulator (ETM) comprising: (a) at least one epigenetic effector domain; operably linked to (b) an endonuclease.
Angelo Leone Lombardo +2 more
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An engineered transcriptional modulator (ETM) comprising: (a) at least one epigenetic effector domain; operably linked to (b) an endonuclease.
Angelo Leone Lombardo +2 more
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Transcriptional gene silencing mutants
Plant Molecular Biology, 2000Genetic approaches to identify molecular components of transcriptional gene silencing (TGS) in plants have yielded several Arabidopsis thaliana mutants and identified the first genes involved. All mutations found affect the maintenance of silencing and reactivate silent genes in trans.
Mittelsten Scheid, O., Paszkowski, Jerzy
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Gene Silencing - Principles And Application
2002Over the last decade, both antisense gene constructs and sense co-suppression gene constructs have been invaluable in the molecular study of biological processes. In plant species ranging from Arabidopsis to rice, these types of constructs have been used to silence genes ranging from transcription factors to metabolic enzymes (1).
Cathryn, Horser +4 more
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Assays for gene silencing in yeast
2002Publisher Summary Silencing in Saccharomyces cerevisiae is a chromatin-mediated repression of genes located within specific chromosomal domains. Each time chromosomal DNA replicates, silent chromatin must be recreated by an orchestrated series of assembly processes and events.
Fred, van Leeuwen, Daniel E, Gottschling
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Posttranscriptional Gene Silencing in Plants
2004Double-stranded RNA when introduced into cells results in severe reduction of the target mRNA. This phenomenon is known as posttranscriptional gene silencing in plants and RNA interference in animals. Hairpin RNA-mediated gene silencing exploits this cellular mechanism. A convenient way of generating hairpin constructs is to use generic vectors such as
Susan Varsha, Wesley +3 more
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