Results 21 to 30 of about 7,098,593 (382)
Cell Reports, 2016 Ewing sarcoma cells depend on the EWS-FLI1 fusion transcription factor for cell survival. Using an assay of EWS-FLI1 activity and genome-wide RNAi screening, we have identified proteins required for the processing of the EWS-FLI1 pre-mRNA.
Patrick J. Grohar +17 more
doaj +1 more source
A universal primer for isolation of fragments of a gene encoding phytoene desaturase for use in virus-induced gene silencing (VIGS) studies [PDF]
, 2010 We have been using Virus-Induced Gene Silencing (VIGS) to test the function of genes that are candidates for involvement in floral senescence. Although VIGS is a powerful tool for assaying the effects of gene silencing in plants, relatively few taxa have
Cai-Zhong, J. +4 more
core +1 more source
Frontiers in Plant Science, 2017 MicroRNAs (miRNAs) are small 20–24nt molecules that have been well studied over the past decade due to their important regulatory roles in different cellular processes.
Muhammad Farooq +7 more
doaj +1 more source
Secondary siRNAs in Plants: Biosynthesis, Various Functions, and Applications in Virology
Frontiers in Plant Science, 2021 The major components of RNA silencing include both transitive and systemic small RNAs, which are technically called secondary sRNAs. Double-stranded RNAs trigger systemic silencing pathways to negatively regulate gene expression.
Neeti Sanan-Mishra +4 more
doaj +1 more source
Enhancement of Recombinant Protein Production in Transgenic Nicotiana benthamiana Plant Cell Suspension Cultures with Co-Cultivation of Agrobacterium Containing Silencing Suppressors. [PDF]
, 2018 We have previously demonstrated that the inducible plant viral vector (CMViva) in transgenic plant cell cultures can significantly improve the productivity of extracellular functional recombinant human alpha-1-antiryspin (rAAT) compared with either a ...
Dandekar, Abhaya M +3 more
core +3 more sources
Potato spindle tuber viroid as Inducer of RNA Silencing in Infected Tomato
Molecular Plant-Microbe Interactions, 2001 Potato spindle tuber viroid (PSTVd), an RNA plant pathogen encoding no known proteins, induces systemic symptoms on tomato plants. We report detection of small RNAs of approximately 25 nucleotides with sequence specificity to PSTVd in infected plants: an
Asuka Itaya +4 more
doaj +1 more source
DNA nanostructures coordinate gene silencing in mature plants
Proceedings of the National Academy of Sciences of the United States of America, 2019 Significance Plant bioengineering will be necessary to sustain plant biology and agriculture, where the delivery of biomolecules such as DNA, RNA, or proteins to plant cells is at the crux of plant biotechnology. Here, we show that DNA nanostructures can
Huan Zhang +8 more
semanticscholar +1 more source
International Journal of Genomics, 2013 Amyotrophic Lateral Sclerosis (ALS) is a rare neurological disease affecting mainly motor neurons and often leads to paralysis and death in extreme cases.
Santosh Shinde +2 more
doaj +1 more source
Epigenetic silencing in transgenic plants
Frontiers in Plant Science, 2015 Epigenetic silencing is a natural phenomenon in which the expression of gene is regulated through modifications of DNA, RNA or histone proteins. It is a mechanism for defending host genomes against the effects of transposable element, viral infection and
Sarma eRajeev Kumar +2 more
doaj +1 more source
siRNA Versus miRNA as Therapeutics for Gene Silencing
Molecular Therapy: Nucleic Acids, 2015 Discovered a little over two decades ago, small interfering RNAs (siRNAs) and microRNAs (miRNAs) are noncoding RNAs with important roles in gene regulation.
J. Lam +3 more
semanticscholar +1 more source