Results 11 to 20 of about 12,260,520 (401)
Gene Targeting in Disease Networks [PDF]
Profiling of whole transcriptomes has become a cornerstone of molecular biology and an invaluable tool for the characterization of clinical phenotypes and the identification of disease subtypes. Analyses of these data are becoming ever more sophisticated
Deborah Weighill+9 more
doaj +8 more sources
Gene targeting in livestock [PDF]
The development of nuclear transfer from tissue culture cells in livestock made it possible in principle to produce animals with subtle, directed genetic changes by in vitro modification of nuclear donor cells. In the short period since nuclear transfer was first performed, gene targeting in livestock has become a reality.
Jim McWhir+2 more
openaire +3 more sources
Inhibition of Murine Cytomegalovirus Infection in Animals by RNase P-Associated External Guide Sequences [PDF]
External guide sequence (EGS) RNAs are associated with ribonuclease P (RNase P), a tRNA processing enzyme, and represent promising agents for gene-targeting applications as they can direct RNase-P-mediated cleavage of a target mRNA.
Wei Li+9 more
doaj +4 more sources
The striatum is involved in action selection, and its disturbance can cause movement disorders. Here, we show that leucine-rich repeats and transmembrane domain 2 (Lrtm2) controls protein sorting in striatal projection systems, and its deficiency causes ...
Misato Ichise+11 more
doaj +1 more source
Gene targeting in plants [PDF]
Although the generation of transgenic plants is now routine, the integration of foreign genetic information has so far been at random sites in the genome. We now present evidence for directed integration into a predicted location in the host plant genome.
Augustyn Bogucki+3 more
openaire +3 more sources
CRISPR/Cas9-Mediated Multi-Allelic Gene Targeting in Sugarcane Confers Herbicide Tolerance
Sugarcane is the source of 80% of the sugar and 26% of the bioethanol produced globally. However, its complex, highly polyploid genome (2n = 100 – 120) impedes crop improvement. Here, we report efficient and reproducible gene targeting (GT) in sugarcane,
Mehmet Tufan Oz+6 more
doaj +1 more source
When it comes to silencing genes in mice, not all approaches are equal. An example published in this issue of Cell (Patrucco et al., 2004) suggests that caution should be used when validating potential drug targets by genetic disruption.
Michael D. Waterfield+3 more
openaire +2 more sources
A technique that involves integration of an exogenous DNA sequence encoding a specific gene into the genome of an organism by homologous recombination.
semanticscholar +1 more source
The effect of primer choice and short read sequences on the outcome of 16S rRNA gene based diversity studies [PDF]
Different regions of the bacterial 16S rRNA gene evolve at different evolutionary rates. The scientific outcome of short read sequencing studies therefore alters with the gene region sequenced.
De Vos, Paul+4 more
core +6 more sources
KDM4B promotes acute myeloid leukemia associated with AML1‐ETO by regulating chromatin accessibility
Epigenetic alterations of chromatin structure affect chromatin accessibility and collaborate with genetic alterations in the development of cancer. Lysine demethylase 4B (KDM4B) has been identified as a JmjC domain‐containing epigenetic modifier that ...
Takeshi Ueda+7 more
doaj +1 more source