Results 281 to 290 of about 12,501,640 (402)

Generation of Sigmar1 conditional knockout mouse using CRISPR-Cas9 gene targeting. [PDF]

Genesis, 2022
Huang L, Xiao H, Xie X, Hu F, Tang F, Smith SB, Gan L.   +6 more
europepmc   +1 more source

Efficient and Heritable Gene Targeting in Tilapia by CRISPR/Cas9

Genetics, 2014
Minghui Li, Huihui Yang, Jiue Zhao, Lingling Fang, Hong‐Juan Shi, Mengru Li, Yun-lv Sun, Xianbo Zhang, Dongneng Jiang, Linyan Zhou, Deshou Wang   +10 more
semanticscholar   +1 more source

RKIP overexpression reduces lung adenocarcinoma aggressiveness and sensitizes cells to EGFR‐targeted therapies

Molecular Oncology, EarlyView.
RKIP, a metastasis suppressor protein, modulates key oncogenic pathways in lung adenocarcinoma. In silico analyses linked low RKIP expression to poor survival. Functional studies revealed RKIP overexpression reduces tumor aggressiveness and enhances sensitivity to EGFR‐targeted therapies, while its loss promotes resistance.
Ana Raquel‐Cunha, Joana Pinheiro, Rui F. Marques, Patrícia Fontão, Diana Cardoso‐Carneiro, Adriana Mendes, Izabela N. F. Gomes, Ana Carolina Laus, Renato J. da Silva‐Oliveira, Rui Manuel Reis, Olga Martinho   +10 more
wiley   +1 more source

CRISPR/Cas9 gene targeting plus nanopore DNA sequencing with the plasmid pBR322 in the classroom. [PDF]

J Microbiol Biol Educ
Wünschiers R, Leidenfrost RM, Holtorf H, Dittrich B, Dürr T, Braun J.   +5 more
europepmc   +1 more source

A joint PCR-based gene-targeting method using electroporation in the pathogenic fungus Trichosporon asahii. [PDF]

AMB Express, 2022
Matsumoto Y, Nagamachi T, Yoshikawa A, Yamada T, Sugita T.   +4 more
europepmc   +1 more source

Nicotinamide N‐methyltransferase promotes drug resistance in lung cancer, as revealed by nascent proteomic profiling

Molecular Oncology, EarlyView.
AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou, Zhen Wang, Fei Yang, Xiao Han, Lei Li, Huadong Liu   +5 more
wiley   +1 more source

Non-viral intron knock-ins for targeted gene integration into human T cells and for T-cell selection. [PDF]

Nat Biomed Eng
Roth TL, Lu J, McClellan A, Kernick C, Takacsi-Nagy O, Satpathy AT.   +5 more
europepmc   +1 more source

Effective gene targeting in rabbits using RNA-guided Cas9 nucleases.

Journal of Molecular Cell Biology, 2014
Dongshan Yang, Jie Xu, Tianqing Zhu, Jianglin Fan, L. Lai, Jifeng Zhang, Y. E. Chen   +6 more
semanticscholar   +1 more source

PARP inhibitors elicit distinct transcriptional programs in homologous recombination competent castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
PARP inhibitors are used to treat a small subset of prostate cancer patients. These studies reveal that PARP1 activity and expression are different between European American and African American prostate cancer tissue samples. Additionally, different PARP inhibitors cause unique and overlapping transcriptional changes, notably, p53 pathway upregulation.
Moriah L. Cunningham, Jasibel Vasquez‐Gonzalez, Samantha M. Barnada, Salome Tchotorlishvili, Latese Jones, Ryan Maguire, Genevieve Lewis, Kinza Rizwan, Jenny Deng, Salma Koachar, Drithi Patel, Hailey Shankle, Tessa Mulders, Namra Ajmal, Charalambos Solomides, Emad S. Alnemri, Teresa F. Alnemri, Ayesha A. Shafi, Leonard G. Gomella, Wm Kevin Kelly, Steven B. McMahon, Matthew J. Schiewer   +21 more
wiley   +1 more source

Comprehensive analysis of off-target and on-target effects resulting from liver-directed CRISPR-Cas9-mediated gene targeting with AAV vectors. [PDF]

Mol Ther Methods Clin Dev
Singh K, Fronza R, Evens H, Chuah MK, VandenDriessche T.   +4 more
europepmc   +1 more source