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Prospects and Possibilities for Gene Transfer Techniques in Insects

, 1993
The ability to analyze and manipulate organisms by classical genetic means has proven to be one of the most powerful tools for studying basic biological questions as well as for addressing important problems in agriculture and medicine. Today we have -available a new set of genetic tools, those of molecular genetics, which allow researchers to more ...
David A. O’Brochta, Alfred M. Handler
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Techniques for gene transfer into neurons

Current Opinion in Neurobiology, 2002
To illuminate the function of the thousands of genes that make up the complexity of the nervous system, it is critical to be able to introduce and express DNA in neurons. Over the past two decades, many gene transfer methods have been developed, including viral vectors, liposomes and electroporation.
Philip, Washbourne, A Kimberley, McAllister   +1 more
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A review of gene transfer techniques

The American Journal of Surgery, 1993
The ability to transfer genetic material from one cell to another provides a novel approach for the investigation and potential treatment of a variety of diseases. Several gene transfer techniques have been developed. Of these, the use of retroviruses as vectors has allowed highly efficient and stable introduction of foreign genes into target mammalian
P C, Watt, M P, Sawicki, E, Passaro
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Gene transfer and antisense nucleic acid techniques

Parasitology Today, 1994
Attempts to suppress a harmful genetic trait by antisense means, or to restore a normal phenotype by gene transfer, attract much publicity. This is especially the case where clinical trials incorporating such methodologies have been initiated, such as antisense oligonucleotide therapies for some types of leukaemia, antisense gene-transfer therapy for a
N, Miller, R G, Vile
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Gene-transfer and plant-regeneration (techniques)

Trends in Biotechnology, 1995
The production of transgenic plants depends on the stable introduction of foreign DNA into the plant genome, followed by regeneration to produce intact plants, and the subsequent expression of the introduced gene(s). A variety of transformation methods have been developed, and this has allowed many of the world's important crop plants to be transformed.
Richard Walden, Ruth Wingender
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New therapeutic approaches based on gene transfer techniques

Springer Seminars in Immunopathology, 1996
The experimental results from the use of gene transfer techniques for cancer therapy are promising but, at the same time, somewhat perplexing. Progress will depend on the further understanding of the biology of tumour interaction with the immune system, as this would be important for the development of optimal strategies for harnessing an effective ...
H, Chong, R G, Vile
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Gene Transfer with DNA Strand Technique and Peripheral Nerve Injuries

Journal of Long-Term Effects of Medical Implants, 2002
Although much progress has been made, current treatments of peripheral nerve injury often result in only partial recovery. Many methods have been used to improve the surgical exploration for injured nerve repair. However, much less is known about the involvement of complex neurochemical interactions. Several neurotrophic factors have been shown to play
Feng, Zhang, William C, Lineaweaver
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Plasmid-mediated gene transfer in neurons using the biolistics technique

Journal of Neuroscience Methods, 1997
Biolistics has been developed as a system for gene delivery into plant cells, but has recently been introduced for transfection into mammalian tissue, including few attempts in neural cells. Basically, in this system the plasmid DNA of interest is coated onto small particles, that are accelerated by a particular driving force.
J E, Biewenga, O H, Destrée, L H, Schrama   +2 more
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Lipospermine-mediated gene transfer technique into murine cultured cortical cells

Journal of Neuroscience Methods, 1997
In order to transfer exogenous DNA into embryonic cortical cells, we have chosen a transfection technique using a synthetic lipospermine (dipalmitoylphosphatidylethanolamylspermine, DPPES) which complexes DNA molecules and allows their penetration into the intracellular compartment.
Sola, Brigitte, C, Staedel, Remy, Jean-Serge, A, Bahr, Behr, Jean-Paul   +4 more
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Techniques of Chromosome-Mediated Gene Transfer

1982
Functional genes can be transferred to eukaryotic cells by incubation with isolated metaphase chromosomes. Uptake occurs by phagocytosis and most of the ingested chromosomal DNA is degraded to small inactive fragments. The frequency of transfer of any selected marker ranges from about 10-5 to 10-7.
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