Results 271 to 280 of about 617,722 (336)

Unveiling leaf rust resistance introgressed from non-progenitor wild species Aegilops kotschyi into hexaploid wheat. [PDF]

Sci Rep
Bai BK, Kaur B, Kaur S, Kaur J, Sharma A, Sri T, Chhuneja P, Kaur S.   +7 more
europepmc   +1 more source

Techniques of DNA-Mediated Gene Transfer for Eukaryotic Cells

, 1982
DNA transformation in bacteria has permitted detailed genetic analysis as well as the implementation of recombinant DNA technology. The utility of manipulation and cloning of genes in bacteria is well recognized. This chapter will concern the manipulation and cloning of genes in eukaryotic systems ranging from yeast to animal systems. Gene manipulation
Arthur P. Bollon, Saul J. Silverstein
openalex   +2 more sources

Prospects and Possibilities for Gene Transfer Techniques in Insects

, 1993
The ability to analyze and manipulate organisms by classical genetic means has proven to be one of the most powerful tools for studying basic biological questions as well as for addressing important problems in agriculture and medicine. Today we have -available a new set of genetic tools, those of molecular genetics, which allow researchers to more ...
David A. O’Brochta, Alfred M. Handler
openalex   +2 more sources

Methods and Role of Embryo Rescue Technique in Alien Gene Transfer

, 2013
Embryo abortion occurs frequently in wide crosses, and thus embryo rescue is required for survival of the next generation. Rescues are performed by either directly transferring the excised embryo to an artificial medium or indirectly through flower (ovary), immature seed (fertilized ovule), or pod (silique) culture.
M. Lulsdorf, A. M. R. Ferrie, Susan M. H. Slater, Hai Ying Yuan   +3 more
openalex   +3 more sources

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Techniques for gene transfer into neurons

Current Opinion in Neurobiology, 2002
To illuminate the function of the thousands of genes that make up the complexity of the nervous system, it is critical to be able to introduce and express DNA in neurons. Over the past two decades, many gene transfer methods have been developed, including viral vectors, liposomes and electroporation.
Philip, Washbourne, A Kimberley, McAllister   +1 more
openaire   +2 more sources

A review of gene transfer techniques

The American Journal of Surgery, 1993
The ability to transfer genetic material from one cell to another provides a novel approach for the investigation and potential treatment of a variety of diseases. Several gene transfer techniques have been developed. Of these, the use of retroviruses as vectors has allowed highly efficient and stable introduction of foreign genes into target mammalian
P C, Watt, M P, Sawicki, E, Passaro
openaire   +2 more sources

Gene transfer and antisense nucleic acid techniques

Parasitology Today, 1994
Attempts to suppress a harmful genetic trait by antisense means, or to restore a normal phenotype by gene transfer, attract much publicity. This is especially the case where clinical trials incorporating such methodologies have been initiated, such as antisense oligonucleotide therapies for some types of leukaemia, antisense gene-transfer therapy for a
N, Miller, R G, Vile
openaire   +2 more sources

Gene-transfer and plant-regeneration (techniques)

Trends in Biotechnology, 1995
The production of transgenic plants depends on the stable introduction of foreign DNA into the plant genome, followed by regeneration to produce intact plants, and the subsequent expression of the introduced gene(s). A variety of transformation methods have been developed, and this has allowed many of the world's important crop plants to be transformed.
Richard Walden, Ruth Wingender
openaire   +1 more source

New therapeutic approaches based on gene transfer techniques

Springer Seminars in Immunopathology, 1996
The experimental results from the use of gene transfer techniques for cancer therapy are promising but, at the same time, somewhat perplexing. Progress will depend on the further understanding of the biology of tumour interaction with the immune system, as this would be important for the development of optimal strategies for harnessing an effective ...
H, Chong, R G, Vile
openaire   +2 more sources

Gene Transfer with DNA Strand Technique and Peripheral Nerve Injuries

Journal of Long-Term Effects of Medical Implants, 2002
Although much progress has been made, current treatments of peripheral nerve injury often result in only partial recovery. Many methods have been used to improve the surgical exploration for injured nerve repair. However, much less is known about the involvement of complex neurochemical interactions. Several neurotrophic factors have been shown to play
Feng, Zhang, William C, Lineaweaver
openaire   +2 more sources