Results 31 to 40 of about 239,079 (262)

Lysophosphatidylcholine as an adjuvant for lentiviral vector mediated gene transfer to airway epithelium: effect of acyl chain length

Respiratory Research, 2010
Background Poor gene transfer efficiency has been a major problem in developing an effective gene therapy for cystic fibrosis (CF) airway disease. Lysophosphatidylcholine (LPC), a natural airway surfactant, can enhance viral gene transfer in animal ...
Anson Don S, Cmielewski Patricia, Parsons David W   +2 more
doaj   +1 more source

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source

Aplication of Gene Transfer in Aquaculture

Jurnal Akuakultur Indonesia, 2003
Recently, global food security has become a hot issue by the public in national as well as out of the country. Aquacultural output will need to be increased several fold in order to meet the rising demands for fish in coming years as the increasing of ...
. Alimuddin, G. Yoshizaki, O. Carman, K. Sumantadinata   +3 more
doaj   +1 more source

Irreversible electroporation is a surgical ablation technique that enhances gene transfer [PDF]

Surgery, 2011
Reversible electroporation has long been used to transfer macromolecules into target cells in the laboratory by using an electric field to induce transient membrane permeability. Recently, the electric field has been modulated to produce permanent membrane permeability and cell death.
Joyce T, Au, Joyce, Wong, Arjun, Mittra, Susanne, Carpenter, Dana, Haddad, Joshua, Carson, Shiva, Jayaraman, Sebastien, Monette, Stephen B, Solomon, Paula, Ezell, Yuman, Fong   +10 more
openaire   +2 more sources

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus)

Frontiers in Genetics, 2018
The sturgeon (Acipenseriformes) is an important farmed species because of its economical value. However, neither gene transfer nor gene editing techniques have been established in sturgeon for molecular breeding and gene functional study until now.
Ji Chen, Wei Wang, Zhaohui Tian, Ying Dong, Tian Dong, Hua Zhu, Zuoyan Zhu, Hongxia Hu, Wei Hu, Wei Hu   +9 more
doaj   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells

Journal of Biomedical Science, 2010
Background Gene transfer into many cell types has been successfully used to develop alternative and adjunct approaches to conventional medical treatment. However, effective transfection of postmitotic neurons remains a challenge.
Wu Chih-Hsien, Sheen-Chen Shyr-Ming, Cheng Jiin-Tsuey, Yang Chien-Hui, Chen Kuan-Hung, Lin Chung-Ren, Sy Wei-Dih, Chen Yi-Shen   +7 more
doaj   +1 more source

Generation of mutant pigs by lipofection-mediated genome editing in embryos

Scientific Reports, 2021
The specificity and efficiency of CRISPR/Cas9 gene-editing systems are determined by several factors, including the mode of delivery, when applied to mammalian embryos.
Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Taro Mito, Sayuri Tomonari, Fuminori Tanihara, Takeshige Otoi   +10 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source