Results 31 to 40 of about 4,360,463 (355)

A simple new approach to variable selection in regression, with application to genetic fine-mapping

bioRxiv, 2018
We introduce a simple new approach to variable selection in linear regression, and to quantifying uncertainty in selected variables. The approach is based on a new model – the “Sum of Single Effects” (SuSiE) model – which comes from writing the sparse ...
Gao Wang, Abhishek K. Sarkar, P. Carbonetto, M. Stephens   +3 more
semanticscholar   +1 more source

Colony polymerase chain reaction of stably transfected Trypanosoma cruzi grown on solid medium [PDF]

Memorias do Instituto Oswaldo Cruz, 2000
Tools for the genetic manipulation of Trypanosoma cruzi are largely unavailable, although several vectors for transfection of epimastigotes and expression of foreign or recombinant genes have been developed. We have previously constructed several plasmid
Wagner G dos Santos, Ivelina Metcheva, Gregory A Buck   +2 more
doaj   +1 more source

Biosafety Evaluation of a Chimeric Adenoviral Vector in Mini-Pigs: Insights into Immune Tolerance and Gene Therapy Potential

Biomedicines
Background: The biosafety of gene therapy products remains a major challenge to their introduction into the clinic. In particular, the problem of immunogenicity of viral vectors is the focus of attention.
Andrei Izmailov, Irina Minyazeva, Vage Markosyan, Zufar Safiullov, Ilnaz Gazizov, Ilnur Salafutdinov, Maria Markelova, Ravil Garifulin, Maksim Shmarov, Denis Logunov, Rustem Islamov, Vadim Pospelov   +11 more
doaj   +1 more source

Safety issues of gene therapy

Онкогематология, 2022
By present time safety problems of gene therapy are widely discussed among scientists of all world. In this review results of clinical researches are summarized; explanations concerning side effects of vectors integration are given; factors that can ...
S. E. Ipatov, S. A. Roumiantsev
doaj  

Beyond Gene Delivery: Strategies to Engineer the Surfaces of Viral Vectors

Biomedicines, 2013
Viral vectors have been extensively studied due to their great transduction efficiency compared to non-viral vectors. These vectors have been used extensively in gene therapy, enabling the comprehension of, not only the advantages of these vectors, but ...
Cristian Capasso, Mari Hirvinen, Vincenzo Cerullo   +2 more
doaj   +1 more source

Genetic characterization of the human relapsing fever spirochete Borrelia miyamotoi in vectors and animal reservoirs of Lyme disease spirochetes in France

Parasites & Vectors, 2014
BackgroundIn France as elsewhere in Europe the most prevalent TBD in humans is Lyme borreliosis, caused by different bacterial species belonging to Borrelia burgdorferi sensu lato complex and transmitted by the most important tick species in France ...
J. Cosson, L. Michelet, J. Chotte, E. Le Naour, Martine Côté, E. Devillers, M. Poulle, D. Huet, M. Galan, J. Geller, S. Moutailler, M. Vayssier‐Taussat   +11 more
semanticscholar   +1 more source

Use of herpes virus amplicon vectors to study brain disorders

BioTechniques, 2005
There is an enormous initiative to establish the genetic basis for disorders of brain function. Unfortunately, genetic intervention is not accomplished easily in the nervous system.
Rachael L. Neve, Kim A. Neve, Eric J. Nestler, William A. Carlezon   +3 more
doaj   +1 more source

Genetic Manipulation of Ticks: A Paradigm Shift in Tick and Tick-Borne Diseases Research

Frontiers in Cellular and Infection Microbiology, 2021
Ticks are obligate hematophagous arthropods that are distributed worldwide and are one of the most important vectors of pathogens affecting humans and animals.
Andrew Nuss, Andrew Nuss, Arvind Sharma, Monika Gulia-Nuss   +3 more
doaj   +1 more source

Delivering genes with human immunodeficiency virus-derived vehicles: still state-of-the-art after 25 years

Journal of Biomedical Science, 2022
Viruses are naturally endowed with the capacity to transfer genetic material between cells. Following early skepticism, engineered viruses have been used to transfer genetic information into thousands of patients, and genetic therapies are currently ...
Jonas Holst Wolff, Jacob Giehm Mikkelsen
doaj   +1 more source

Assessment of Genetic Shielding for Adenovirus Vectors [PDF]

The Open Gene Therapy Journal, 2009
Development of adenovirus (Ad) vectors in the clinical context has highlighted that vector efficacy may be limited by the host humoral response due to pre-existing titers of neutralizing antibodies against the vector itself in humans. Further, multiple dosing of Ad vectors based on serotype 5 would be limited.
Hui-Chen Hsu, Myunghee Kim, Jian Chen, Imre Kovesdi, Aleksandr Krendelshchikov, David T. Curiel, Susan J. Hedley, John D. Mountz   +7 more
openaire   +2 more sources